The Anaphase Promoting Complex/Cyclosome Subunit 11 and Its Role in Organ Size and Plant Development
The anaphase promoting complex/cyclosome (APC/C), a member of the E3 ubiquitin ligase family, plays an important role in recognizing the substrates to be ubiquitylated. Progression of anaphase, and therefore, of the cell cycle, is coordinated through cyclin degradation cycles dependent on proteolysi...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-11-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2021.563760/full |
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| author | Rodrigo Porto Schwedersky Marina de Lyra Soriano Saleme Ingrid Andrade Rocha Patricia da Fonseca Montessoro Adriana Silva Hemerly Nubia Barbosa Eloy Paulo Cavalcanti Gomes Ferreira |
| author_facet | Rodrigo Porto Schwedersky Marina de Lyra Soriano Saleme Ingrid Andrade Rocha Patricia da Fonseca Montessoro Adriana Silva Hemerly Nubia Barbosa Eloy Paulo Cavalcanti Gomes Ferreira |
| author_sort | Rodrigo Porto Schwedersky |
| collection | DOAJ |
| description | The anaphase promoting complex/cyclosome (APC/C), a member of the E3 ubiquitin ligase family, plays an important role in recognizing the substrates to be ubiquitylated. Progression of anaphase, and therefore, of the cell cycle, is coordinated through cyclin degradation cycles dependent on proteolysis triggered by APC/C. The APC/C activity depends on the formation of a pocket comprising the catalytic subunits, APC2, APC11, and APC10. Among these, the role of APC11 outside the cell division cycle is poorly understood. Therefore, the goal of this work was to analyze the function of APC11 during plant development by characterizing apc11 knock-down mutant lines. Accordingly, we observed decreased apc11 expression in the mutant lines, followed by a reduction in meristem root size based on the cortical cell length, and an overall size diminishment throughout the development. Additionally, crosses of apc11-1 and amiR-apc11 with plants carrying a WUSCHEL-RELATED HOMEOBOX5 (WOX5) fluorescent marker showed a weakening of the green fluorescent protein-positive cells in the Quiescent Center. Moreover, plants with apc11-1 show a decreased leaf area, together with a decrease in the cell area when the shoot development was observed by kinematics analysis. Finally, we observed a decreased APC/C activity in the root and shoot meristems in crosses of pCYCB1;1:D-box-GUS with apc11-1 plants. Our results indicate that APC11 is important in the early stages of development, mediating meristematic architecture through APC/C activity affecting the overall plant growth. |
| first_indexed | 2024-12-19T02:25:17Z |
| format | Article |
| id | doaj.art-608cf35d98684091bc4b964d288bc943 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-19T02:25:17Z |
| publishDate | 2021-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-608cf35d98684091bc4b964d288bc9432022-12-21T20:39:57ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2021-11-011210.3389/fpls.2021.563760563760The Anaphase Promoting Complex/Cyclosome Subunit 11 and Its Role in Organ Size and Plant DevelopmentRodrigo Porto Schwedersky0Marina de Lyra Soriano Saleme1Ingrid Andrade Rocha2Patricia da Fonseca Montessoro3Adriana Silva Hemerly4Nubia Barbosa Eloy5Paulo Cavalcanti Gomes Ferreira6Laboratorio de Biologia Molecular de Plantas, Instituto de Bioquímica Médica Universidade Federal do Rio de Janeiro, Rio de Janeiro, BrazilDepartment of Biological Sciences, Escola Superior de Agricultura ‘Luiz de Queiroz’, University of São Paulo, Piracicaba, BrazilDepartment of Biological Sciences, Escola Superior de Agricultura ‘Luiz de Queiroz’, University of São Paulo, Piracicaba, BrazilLaboratorio de Biologia Molecular de Plantas, Instituto de Bioquímica Médica Universidade Federal do Rio de Janeiro, Rio de Janeiro, BrazilLaboratorio de Biologia Molecular de Plantas, Instituto de Bioquímica Médica Universidade Federal do Rio de Janeiro, Rio de Janeiro, BrazilDepartment of Biological Sciences, Escola Superior de Agricultura ‘Luiz de Queiroz’, University of São Paulo, Piracicaba, BrazilLaboratorio de Biologia Molecular de Plantas, Instituto de Bioquímica Médica Universidade Federal do Rio de Janeiro, Rio de Janeiro, BrazilThe anaphase promoting complex/cyclosome (APC/C), a member of the E3 ubiquitin ligase family, plays an important role in recognizing the substrates to be ubiquitylated. Progression of anaphase, and therefore, of the cell cycle, is coordinated through cyclin degradation cycles dependent on proteolysis triggered by APC/C. The APC/C activity depends on the formation of a pocket comprising the catalytic subunits, APC2, APC11, and APC10. Among these, the role of APC11 outside the cell division cycle is poorly understood. Therefore, the goal of this work was to analyze the function of APC11 during plant development by characterizing apc11 knock-down mutant lines. Accordingly, we observed decreased apc11 expression in the mutant lines, followed by a reduction in meristem root size based on the cortical cell length, and an overall size diminishment throughout the development. Additionally, crosses of apc11-1 and amiR-apc11 with plants carrying a WUSCHEL-RELATED HOMEOBOX5 (WOX5) fluorescent marker showed a weakening of the green fluorescent protein-positive cells in the Quiescent Center. Moreover, plants with apc11-1 show a decreased leaf area, together with a decrease in the cell area when the shoot development was observed by kinematics analysis. Finally, we observed a decreased APC/C activity in the root and shoot meristems in crosses of pCYCB1;1:D-box-GUS with apc11-1 plants. Our results indicate that APC11 is important in the early stages of development, mediating meristematic architecture through APC/C activity affecting the overall plant growth.https://www.frontiersin.org/articles/10.3389/fpls.2021.563760/fullplant developmentcell cycleanaphase promoting complex (APC)organ sizemeristem cells |
| spellingShingle | Rodrigo Porto Schwedersky Marina de Lyra Soriano Saleme Ingrid Andrade Rocha Patricia da Fonseca Montessoro Adriana Silva Hemerly Nubia Barbosa Eloy Paulo Cavalcanti Gomes Ferreira The Anaphase Promoting Complex/Cyclosome Subunit 11 and Its Role in Organ Size and Plant Development Frontiers in Plant Science plant development cell cycle anaphase promoting complex (APC) organ size meristem cells |
| title | The Anaphase Promoting Complex/Cyclosome Subunit 11 and Its Role in Organ Size and Plant Development |
| title_full | The Anaphase Promoting Complex/Cyclosome Subunit 11 and Its Role in Organ Size and Plant Development |
| title_fullStr | The Anaphase Promoting Complex/Cyclosome Subunit 11 and Its Role in Organ Size and Plant Development |
| title_full_unstemmed | The Anaphase Promoting Complex/Cyclosome Subunit 11 and Its Role in Organ Size and Plant Development |
| title_short | The Anaphase Promoting Complex/Cyclosome Subunit 11 and Its Role in Organ Size and Plant Development |
| title_sort | anaphase promoting complex cyclosome subunit 11 and its role in organ size and plant development |
| topic | plant development cell cycle anaphase promoting complex (APC) organ size meristem cells |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2021.563760/full |
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