Mammalian pumilio proteins control cellular morphology, migration, and adhesion
Abstract Pumilio proteins are RNA-binding proteins that control mRNA translation and stability by binding to the 3’ UTR of target mRNAs. Mammals have two canonical Pumilio proteins, PUM1 and PUM2, which are known to act in many biological processes, including embryonic development, neurogenesis, cel...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-02-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-30004-4 |
| _version_ | 1797864692436172800 |
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| author | Erin L. Sternburg Jordan J. Lillibridge Rattapol Phandthong Fedor V. Karginov |
| author_facet | Erin L. Sternburg Jordan J. Lillibridge Rattapol Phandthong Fedor V. Karginov |
| author_sort | Erin L. Sternburg |
| collection | DOAJ |
| description | Abstract Pumilio proteins are RNA-binding proteins that control mRNA translation and stability by binding to the 3’ UTR of target mRNAs. Mammals have two canonical Pumilio proteins, PUM1 and PUM2, which are known to act in many biological processes, including embryonic development, neurogenesis, cell cycle regulation and genomic stability. Here, we characterized a new role of both PUM1 and PUM2 in regulating cell morphology, migration, and adhesion in T-REx-293 cells, in addition to previously known defects in growth rate. Gene ontology analysis of differentially expressed genes in PUM double knockout (PDKO) cells for both cellular component and biological process showed enrichment in categories related to adhesion and migration. PDKO cells had a collective cell migration rate significantly lower than that of WT cells and displayed changes in actin morphology. In addition, during growth, PDKO cells aggregated into clusters (clumps) due to an inability to escape cell–cell contacts. Addition of extracellular matrix (Matrigel) alleviated the clumping phenotype. Collagen IV (ColIV), a major component of Matrigel, was shown to be the driving force in allowing PDKO cells to monolayer appropriately, however, ColIV protein levels remained unperturbed in PDKO cells. This study characterizes a novel cellular phenotype associated with cellular morphology, migration, and adhesion which can aid in developing better models for PUM function in both developmental processes and disease. |
| first_indexed | 2024-04-09T22:57:14Z |
| format | Article |
| id | doaj.art-608e3f380efe43db9b76d7d0545de62d |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-09T22:57:14Z |
| publishDate | 2023-02-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-608e3f380efe43db9b76d7d0545de62d2023-03-22T11:13:01ZengNature PortfolioScientific Reports2045-23222023-02-0113111110.1038/s41598-023-30004-4Mammalian pumilio proteins control cellular morphology, migration, and adhesionErin L. Sternburg0Jordan J. Lillibridge1Rattapol Phandthong2Fedor V. Karginov3Department of Molecular, Cell and Systems Biology, University of California at RiversideDepartment of Molecular, Cell and Systems Biology, University of California at RiversideDepartment of Molecular, Cell and Systems Biology, University of California at RiversideDepartment of Molecular, Cell and Systems Biology, University of California at RiversideAbstract Pumilio proteins are RNA-binding proteins that control mRNA translation and stability by binding to the 3’ UTR of target mRNAs. Mammals have two canonical Pumilio proteins, PUM1 and PUM2, which are known to act in many biological processes, including embryonic development, neurogenesis, cell cycle regulation and genomic stability. Here, we characterized a new role of both PUM1 and PUM2 in regulating cell morphology, migration, and adhesion in T-REx-293 cells, in addition to previously known defects in growth rate. Gene ontology analysis of differentially expressed genes in PUM double knockout (PDKO) cells for both cellular component and biological process showed enrichment in categories related to adhesion and migration. PDKO cells had a collective cell migration rate significantly lower than that of WT cells and displayed changes in actin morphology. In addition, during growth, PDKO cells aggregated into clusters (clumps) due to an inability to escape cell–cell contacts. Addition of extracellular matrix (Matrigel) alleviated the clumping phenotype. Collagen IV (ColIV), a major component of Matrigel, was shown to be the driving force in allowing PDKO cells to monolayer appropriately, however, ColIV protein levels remained unperturbed in PDKO cells. This study characterizes a novel cellular phenotype associated with cellular morphology, migration, and adhesion which can aid in developing better models for PUM function in both developmental processes and disease.https://doi.org/10.1038/s41598-023-30004-4 |
| spellingShingle | Erin L. Sternburg Jordan J. Lillibridge Rattapol Phandthong Fedor V. Karginov Mammalian pumilio proteins control cellular morphology, migration, and adhesion Scientific Reports |
| title | Mammalian pumilio proteins control cellular morphology, migration, and adhesion |
| title_full | Mammalian pumilio proteins control cellular morphology, migration, and adhesion |
| title_fullStr | Mammalian pumilio proteins control cellular morphology, migration, and adhesion |
| title_full_unstemmed | Mammalian pumilio proteins control cellular morphology, migration, and adhesion |
| title_short | Mammalian pumilio proteins control cellular morphology, migration, and adhesion |
| title_sort | mammalian pumilio proteins control cellular morphology migration and adhesion |
| url | https://doi.org/10.1038/s41598-023-30004-4 |
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