Lipidomic Impacts of an Obesogenic Diet Upon Lewis Lung Carcinoma in Mice
Metabolic reprogramming of lipid metabolism is a hallmark of cancer. Consumption of a high-fat obesogenic diet enhances spontaneous metastasis using a Lewis lung carcinoma (LLC) model. In order to gain further insights into the mechanisms by which dietary fats impact cancer progression, we conducted...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2018-05-01
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| Series: | Frontiers in Oncology |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fonc.2018.00134/full |
| _version_ | 1819015063721213952 |
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| author | Sneha Sundaram Petr Žáček Michael R. Bukowski Aaron A. Mehus Lin Yan Matthew J. Picklo Matthew J. Picklo |
| author_facet | Sneha Sundaram Petr Žáček Michael R. Bukowski Aaron A. Mehus Lin Yan Matthew J. Picklo Matthew J. Picklo |
| author_sort | Sneha Sundaram |
| collection | DOAJ |
| description | Metabolic reprogramming of lipid metabolism is a hallmark of cancer. Consumption of a high-fat obesogenic diet enhances spontaneous metastasis using a Lewis lung carcinoma (LLC) model. In order to gain further insights into the mechanisms by which dietary fats impact cancer progression, we conducted a lipidomic analysis of primary tumors originated from LLC from mice fed with a standard AIN93G diet or a soybean oil-based high-fat diet (HFD). Hierarchical clustering heatmap analysis of phosphatidylcholine (PC) lipids and phosphatidylethanolamine (PE) lipids demonstrated an increase in polyunsaturated fatty acids (PUFA)-containing phospholipids and a decrease in monounsaturated fatty acids (MUFA)-containing lipids in tumors from mice fed the HFD. The quantities of 51 PC and 24 PE lipids differed in primary tumors of LLC from mice fed the control diet and the HFD. Analysis of triacylglycerol (TAG) lipids identified differences in 32 TAG (by brutto structure) between the two groups; TAG analysis by neutral loss identified 46 PUFA-containing TAG species that were higher in mice fed with the HFD than in the controls. Intake of the HFD did not alter the expression of the de novo lipogenesis enzymes (fatty acid synthase, acetyl-CoA carboxylase-1, and stearoyl-CoA desaturase-1). Our results demonstrate that the dietary fatty acid composition of the HFD is reflected in the higher order lipidomic composition of primary tumors. Subsequent studies are needed to investigate how these lipidomic changes may be used for targeted dietary intervention to reduce tumor growth and malignant progression. |
| first_indexed | 2024-12-21T02:25:47Z |
| format | Article |
| id | doaj.art-608edb010a8344efa33ee160fc969336 |
| institution | Directory Open Access Journal |
| issn | 2234-943X |
| language | English |
| last_indexed | 2024-12-21T02:25:47Z |
| publishDate | 2018-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Oncology |
| spelling | doaj.art-608edb010a8344efa33ee160fc9693362022-12-21T19:19:01ZengFrontiers Media S.A.Frontiers in Oncology2234-943X2018-05-01810.3389/fonc.2018.00134364300Lipidomic Impacts of an Obesogenic Diet Upon Lewis Lung Carcinoma in MiceSneha Sundaram0Petr Žáček1Michael R. Bukowski2Aaron A. Mehus3Lin Yan4Matthew J. Picklo5Matthew J. Picklo6USDA-ARS Grand Forks Human Nutrition Research Center, Grand Forks, ND, United StatesUSDA-ARS Grand Forks Human Nutrition Research Center, Grand Forks, ND, United StatesUSDA-ARS Grand Forks Human Nutrition Research Center, Grand Forks, ND, United StatesUSDA-ARS Grand Forks Human Nutrition Research Center, Grand Forks, ND, United StatesUSDA-ARS Grand Forks Human Nutrition Research Center, Grand Forks, ND, United StatesUSDA-ARS Grand Forks Human Nutrition Research Center, Grand Forks, ND, United StatesDepartment of Chemistry, University of North Dakota, Grand Forks, ND, United StatesMetabolic reprogramming of lipid metabolism is a hallmark of cancer. Consumption of a high-fat obesogenic diet enhances spontaneous metastasis using a Lewis lung carcinoma (LLC) model. In order to gain further insights into the mechanisms by which dietary fats impact cancer progression, we conducted a lipidomic analysis of primary tumors originated from LLC from mice fed with a standard AIN93G diet or a soybean oil-based high-fat diet (HFD). Hierarchical clustering heatmap analysis of phosphatidylcholine (PC) lipids and phosphatidylethanolamine (PE) lipids demonstrated an increase in polyunsaturated fatty acids (PUFA)-containing phospholipids and a decrease in monounsaturated fatty acids (MUFA)-containing lipids in tumors from mice fed the HFD. The quantities of 51 PC and 24 PE lipids differed in primary tumors of LLC from mice fed the control diet and the HFD. Analysis of triacylglycerol (TAG) lipids identified differences in 32 TAG (by brutto structure) between the two groups; TAG analysis by neutral loss identified 46 PUFA-containing TAG species that were higher in mice fed with the HFD than in the controls. Intake of the HFD did not alter the expression of the de novo lipogenesis enzymes (fatty acid synthase, acetyl-CoA carboxylase-1, and stearoyl-CoA desaturase-1). Our results demonstrate that the dietary fatty acid composition of the HFD is reflected in the higher order lipidomic composition of primary tumors. Subsequent studies are needed to investigate how these lipidomic changes may be used for targeted dietary intervention to reduce tumor growth and malignant progression.http://journal.frontiersin.org/article/10.3389/fonc.2018.00134/fulllipidomicsLewis lung carcinomaprimary tumorhigh-fat dietmice |
| spellingShingle | Sneha Sundaram Petr Žáček Michael R. Bukowski Aaron A. Mehus Lin Yan Matthew J. Picklo Matthew J. Picklo Lipidomic Impacts of an Obesogenic Diet Upon Lewis Lung Carcinoma in Mice Frontiers in Oncology lipidomics Lewis lung carcinoma primary tumor high-fat diet mice |
| title | Lipidomic Impacts of an Obesogenic Diet Upon Lewis Lung Carcinoma in Mice |
| title_full | Lipidomic Impacts of an Obesogenic Diet Upon Lewis Lung Carcinoma in Mice |
| title_fullStr | Lipidomic Impacts of an Obesogenic Diet Upon Lewis Lung Carcinoma in Mice |
| title_full_unstemmed | Lipidomic Impacts of an Obesogenic Diet Upon Lewis Lung Carcinoma in Mice |
| title_short | Lipidomic Impacts of an Obesogenic Diet Upon Lewis Lung Carcinoma in Mice |
| title_sort | lipidomic impacts of an obesogenic diet upon lewis lung carcinoma in mice |
| topic | lipidomics Lewis lung carcinoma primary tumor high-fat diet mice |
| url | http://journal.frontiersin.org/article/10.3389/fonc.2018.00134/full |
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