Dietary Use of Methionine Sources and <i>Bacillus amyloliquefaciens</i> CECT 5940 Influences Growth Performance, Hepatopancreatic Histology, Digestion, Immunity, and Digestive Microbiota of <i>Litopenaeus vannamei</i> Fed Reduced Fishmeal Diets
An 8-week feeding trial investigated the effect of Fishmeal (FM) replacement by soybean meal (SBM) and poultry by-product meal (PBM) in diets supplemented with DL-Met, MET-MET (AQUAVI<sup>®</sup>), <i>Bacillus amyloliquefaciens</i> CECT 5940 (ECOBIOL<sup>®</sup>)...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-12-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/13/1/43 |
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| author | Ramón Casillas-Hernández Jose Reyes Gonzalez-Galaviz Libia Zulema Rodriguez-Anaya Juan Carlos Gil-Núñez María del Carmen Rodríguez-Jaramillo |
| author_facet | Ramón Casillas-Hernández Jose Reyes Gonzalez-Galaviz Libia Zulema Rodriguez-Anaya Juan Carlos Gil-Núñez María del Carmen Rodríguez-Jaramillo |
| author_sort | Ramón Casillas-Hernández |
| collection | DOAJ |
| description | An 8-week feeding trial investigated the effect of Fishmeal (FM) replacement by soybean meal (SBM) and poultry by-product meal (PBM) in diets supplemented with DL-Met, MET-MET (AQUAVI<sup>®</sup>), <i>Bacillus amyloliquefaciens</i> CECT 5940 (ECOBIOL<sup>®</sup>) and their combinations on growth performance and health of juvenile <i>Litopenaeus vannamei</i>. A total of six experimental diets were formulated according to <i>L. vannamei</i> nutritional requirements. A total of 480 shrimp (0.30 ± 0.04 g) were randomly distributed into 24 tanks (4 repetitions/each diet, 20 shrimp/tank). Shrimp were fed with control diet (CD; 200 g/Kg fishmeal) and five diets with 50% FM replacement supplemented with different methionine sources, probiotic (<i>B. amyloliquefaciens</i> CECT 5940) and their combinations: D1 (0.13% DL-MET), D2 (0.06% MET-MET), D3 (0.19% MET-MET), D4 (0.13% DL-MET plus 0.10% <i>B. amyloliquefaciens</i> CECT 5940 and D5 (0.06% MET-MET plus 0.10% <i>B. amyloliquefaciens</i> CECT 5940). Shrimp fed D3 and D5 had significantly higher final, weekly weight gain, and final biomass compared to shrimp fed CD (<i>p</i> < 0.05). Shrimp fed D2 to D5 increased the hepatopancreas epithelial cell height (<i>p</i> < 0.05). Digestive enzymatic activities were significantly increased in shrimp hepatopancreas’ fed D3 (<i>p</i> < 0.05). Meanwhile, shrimp fed D1 had significant downregulation of immune-related genes (<i>p</i> < 0.05). Moreover, shrimp fed D3 and D5 increased the abundance of beneficial prokaryotic microorganisms such as <i>Pseudoalteromonas</i> and <i>Demequina</i> related to carbohydrate metabolism and immune stimulation. Also, shrimp fed D3 and D5 increased the abundance of beneficial eukaryotic microorganism as <i>Aurantiochytrium</i> and <i>Aplanochytrium</i> were related to eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) production which plays a role in growth promoting or boosting the immunity of aquatic organisms. Therefore, fishmeal could be partially substituted up to 50% by SBM and PBM in diets supplemented with 0.19% MET-MET (AQUAVI<sup>®</sup>) or 0.06% MET-MET (AQUAVI<sup>®</sup>) plus 0.10% <i>B. amyloliquefaciens</i> CECT 5940 (ECOBIOL<sup>®</sup>) and improve the productive performance, health, and immunity of white shrimp. Further research is necessary to investigate synergistic effects of amino acids and probiotics in farmed shrimp diets, as well as to evaluate how SBM and PBM influence the fatty acid composition of reduced fishmeal diets and shrimp muscle quality. Nevertheless, this information could be interesting to develop low fishmeal feeds for aquaculture without affecting the growth and welfare of aquatic organisms. |
| first_indexed | 2024-03-11T10:12:46Z |
| format | Article |
| id | doaj.art-608f90247e074cddbd08b21d6c2bf689 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-11T10:12:46Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-608f90247e074cddbd08b21d6c2bf6892023-11-16T14:18:24ZengMDPI AGAnimals2076-26152022-12-011314310.3390/ani13010043Dietary Use of Methionine Sources and <i>Bacillus amyloliquefaciens</i> CECT 5940 Influences Growth Performance, Hepatopancreatic Histology, Digestion, Immunity, and Digestive Microbiota of <i>Litopenaeus vannamei</i> Fed Reduced Fishmeal DietsRamón Casillas-Hernández0Jose Reyes Gonzalez-Galaviz1Libia Zulema Rodriguez-Anaya2Juan Carlos Gil-Núñez3María del Carmen Rodríguez-Jaramillo4Departamento de Ciencias Agronómicas y Veterinarias, Instituto Tecnológico de Sonora, 5 de Febrero 818 Sur, Colonia Centro, Cd. Obregón 85000, Sonora, MexicoCONACYT-Instituto Tecnológico de Sonora, 5 de Febrero 818 Sur, Colonia Centro, Cd. Obregón 85000, Sonora, MexicoCONACYT-Instituto Tecnológico de Sonora, 5 de Febrero 818 Sur, Colonia Centro, Cd. Obregón 85000, Sonora, MexicoDepartamento de Ciencias Agronómicas y Veterinarias, Instituto Tecnológico de Sonora, 5 de Febrero 818 Sur, Colonia Centro, Cd. Obregón 85000, Sonora, MexicoCentro de Investigaciones Biológicas del Noroeste, Calle Instituto Politécnico Nacional No. 195, La Paz 23096, Baja California Sur, MexicoAn 8-week feeding trial investigated the effect of Fishmeal (FM) replacement by soybean meal (SBM) and poultry by-product meal (PBM) in diets supplemented with DL-Met, MET-MET (AQUAVI<sup>®</sup>), <i>Bacillus amyloliquefaciens</i> CECT 5940 (ECOBIOL<sup>®</sup>) and their combinations on growth performance and health of juvenile <i>Litopenaeus vannamei</i>. A total of six experimental diets were formulated according to <i>L. vannamei</i> nutritional requirements. A total of 480 shrimp (0.30 ± 0.04 g) were randomly distributed into 24 tanks (4 repetitions/each diet, 20 shrimp/tank). Shrimp were fed with control diet (CD; 200 g/Kg fishmeal) and five diets with 50% FM replacement supplemented with different methionine sources, probiotic (<i>B. amyloliquefaciens</i> CECT 5940) and their combinations: D1 (0.13% DL-MET), D2 (0.06% MET-MET), D3 (0.19% MET-MET), D4 (0.13% DL-MET plus 0.10% <i>B. amyloliquefaciens</i> CECT 5940 and D5 (0.06% MET-MET plus 0.10% <i>B. amyloliquefaciens</i> CECT 5940). Shrimp fed D3 and D5 had significantly higher final, weekly weight gain, and final biomass compared to shrimp fed CD (<i>p</i> < 0.05). Shrimp fed D2 to D5 increased the hepatopancreas epithelial cell height (<i>p</i> < 0.05). Digestive enzymatic activities were significantly increased in shrimp hepatopancreas’ fed D3 (<i>p</i> < 0.05). Meanwhile, shrimp fed D1 had significant downregulation of immune-related genes (<i>p</i> < 0.05). Moreover, shrimp fed D3 and D5 increased the abundance of beneficial prokaryotic microorganisms such as <i>Pseudoalteromonas</i> and <i>Demequina</i> related to carbohydrate metabolism and immune stimulation. Also, shrimp fed D3 and D5 increased the abundance of beneficial eukaryotic microorganism as <i>Aurantiochytrium</i> and <i>Aplanochytrium</i> were related to eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) production which plays a role in growth promoting or boosting the immunity of aquatic organisms. Therefore, fishmeal could be partially substituted up to 50% by SBM and PBM in diets supplemented with 0.19% MET-MET (AQUAVI<sup>®</sup>) or 0.06% MET-MET (AQUAVI<sup>®</sup>) plus 0.10% <i>B. amyloliquefaciens</i> CECT 5940 (ECOBIOL<sup>®</sup>) and improve the productive performance, health, and immunity of white shrimp. Further research is necessary to investigate synergistic effects of amino acids and probiotics in farmed shrimp diets, as well as to evaluate how SBM and PBM influence the fatty acid composition of reduced fishmeal diets and shrimp muscle quality. Nevertheless, this information could be interesting to develop low fishmeal feeds for aquaculture without affecting the growth and welfare of aquatic organisms.https://www.mdpi.com/2076-2615/13/1/43<i>Litopenaeus vannamei</i>shrimp nutritionfishmeal replacementmethionineprobioticsperformance |
| spellingShingle | Ramón Casillas-Hernández Jose Reyes Gonzalez-Galaviz Libia Zulema Rodriguez-Anaya Juan Carlos Gil-Núñez María del Carmen Rodríguez-Jaramillo Dietary Use of Methionine Sources and <i>Bacillus amyloliquefaciens</i> CECT 5940 Influences Growth Performance, Hepatopancreatic Histology, Digestion, Immunity, and Digestive Microbiota of <i>Litopenaeus vannamei</i> Fed Reduced Fishmeal Diets Animals <i>Litopenaeus vannamei</i> shrimp nutrition fishmeal replacement methionine probiotics performance |
| title | Dietary Use of Methionine Sources and <i>Bacillus amyloliquefaciens</i> CECT 5940 Influences Growth Performance, Hepatopancreatic Histology, Digestion, Immunity, and Digestive Microbiota of <i>Litopenaeus vannamei</i> Fed Reduced Fishmeal Diets |
| title_full | Dietary Use of Methionine Sources and <i>Bacillus amyloliquefaciens</i> CECT 5940 Influences Growth Performance, Hepatopancreatic Histology, Digestion, Immunity, and Digestive Microbiota of <i>Litopenaeus vannamei</i> Fed Reduced Fishmeal Diets |
| title_fullStr | Dietary Use of Methionine Sources and <i>Bacillus amyloliquefaciens</i> CECT 5940 Influences Growth Performance, Hepatopancreatic Histology, Digestion, Immunity, and Digestive Microbiota of <i>Litopenaeus vannamei</i> Fed Reduced Fishmeal Diets |
| title_full_unstemmed | Dietary Use of Methionine Sources and <i>Bacillus amyloliquefaciens</i> CECT 5940 Influences Growth Performance, Hepatopancreatic Histology, Digestion, Immunity, and Digestive Microbiota of <i>Litopenaeus vannamei</i> Fed Reduced Fishmeal Diets |
| title_short | Dietary Use of Methionine Sources and <i>Bacillus amyloliquefaciens</i> CECT 5940 Influences Growth Performance, Hepatopancreatic Histology, Digestion, Immunity, and Digestive Microbiota of <i>Litopenaeus vannamei</i> Fed Reduced Fishmeal Diets |
| title_sort | dietary use of methionine sources and i bacillus amyloliquefaciens i cect 5940 influences growth performance hepatopancreatic histology digestion immunity and digestive microbiota of i litopenaeus vannamei i fed reduced fishmeal diets |
| topic | <i>Litopenaeus vannamei</i> shrimp nutrition fishmeal replacement methionine probiotics performance |
| url | https://www.mdpi.com/2076-2615/13/1/43 |
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