The Features of a Humoral Immune Response Development during the Acute Phase of ixodes Tick-Borne Borreliosis (Based on Multiplex Analysis with the Immunochip)
The features of development of IgM or IgG immune responses to Borrelia antigens (C6, OspC and VlsE) were studied in 155 patients with localized and disseminated stages of disease with Ixodid Tick-borne Borrelioses (ITBB), the cases of mixed infection with ITBB and human granulocytic anaplasmosis (HG...
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| Format: | Article |
| Language: | Russian |
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Numikom LLC
2015-02-01
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| Series: | Эпидемиология и вакцинопрофилактика |
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| Online Access: | https://www.epidemvac.ru/jour/article/view/8 |
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| author | V. G. Pomelova E. I. Korenberg T. I. Kuznetsova N. S. Osin |
| author_facet | V. G. Pomelova E. I. Korenberg T. I. Kuznetsova N. S. Osin |
| author_sort | V. G. Pomelova |
| collection | DOAJ |
| description | The features of development of IgM or IgG immune responses to Borrelia antigens (C6, OspC and VlsE) were studied in 155 patients with localized and disseminated stages of disease with Ixodid Tick-borne Borrelioses (ITBB), the cases of mixed infection with ITBB and human granulocytic anaplasmosis (HGA) including. Patient sera (n = 373) were examined by a multiplex microarray immunoassay test (PHOSPHANTM). At the localized stage of the disease, a positive PHOSPHAN reaction with C6 from B. burgdorferi was observed significantly more frequently in tests for IgG than for IgM at almost all times after tick bite, except for the first week. The frequency of positive samples in tests for both IgM and IgG was higher than for IgG alone, although the difference lacked statistical significance. Positive reactions with OspC or VlsE were observed only in the samples from patients that were also positive for IgM and/or IgG to C6. Therefore, additional detection of IgM to recombinant proteins did not improve significantly the overall sensitivity of the latter PHOSPHAN variant. As compared with ITBB monoinfection, the frequency of positive samples in patients with mixed ITBB + HGA infection was significantly higher (p < 0.05). At the disseminated stage of the disease, the proportion of positive reactions with any of Borrelia antigens for IgM was comparable or higher than for C6-IgG that resulted in a substantial number of patients with negative reactions for IgG; this necessitates the use of alternative methods (e.g., PCR) to exclude false-positive serologic results. |
| first_indexed | 2024-04-10T02:52:05Z |
| format | Article |
| id | doaj.art-60901e5cd3f748418ac5dcb60b8365e0 |
| institution | Directory Open Access Journal |
| issn | 2073-3046 2619-0494 |
| language | Russian |
| last_indexed | 2024-04-10T02:52:05Z |
| publishDate | 2015-02-01 |
| publisher | Numikom LLC |
| record_format | Article |
| series | Эпидемиология и вакцинопрофилактика |
| spelling | doaj.art-60901e5cd3f748418ac5dcb60b8365e02023-03-13T07:43:11ZrusNumikom LLCЭпидемиология и вакцинопрофилактика2073-30462619-04942015-02-01141202710.31631/2073-3046-2015-14-1-20-277The Features of a Humoral Immune Response Development during the Acute Phase of ixodes Tick-Borne Borreliosis (Based on Multiplex Analysis with the Immunochip)V. G. Pomelova0E. I. Korenberg1T. I. Kuznetsova2N. S. Osin3ФГУП «Государственный научно-исследовательский институт биологического приборостроения» ФМБА РоссииФГБУ «ФНИЦ эпидемиологии и микробиологии имени почетного академика Н.Ф. Гамалеи» Минздрава РоссииГБУЗ Пермского края «Пермская краевая клиническая инфекционная больница»ЗАО «Иммуноскрин»The features of development of IgM or IgG immune responses to Borrelia antigens (C6, OspC and VlsE) were studied in 155 patients with localized and disseminated stages of disease with Ixodid Tick-borne Borrelioses (ITBB), the cases of mixed infection with ITBB and human granulocytic anaplasmosis (HGA) including. Patient sera (n = 373) were examined by a multiplex microarray immunoassay test (PHOSPHANTM). At the localized stage of the disease, a positive PHOSPHAN reaction with C6 from B. burgdorferi was observed significantly more frequently in tests for IgG than for IgM at almost all times after tick bite, except for the first week. The frequency of positive samples in tests for both IgM and IgG was higher than for IgG alone, although the difference lacked statistical significance. Positive reactions with OspC or VlsE were observed only in the samples from patients that were also positive for IgM and/or IgG to C6. Therefore, additional detection of IgM to recombinant proteins did not improve significantly the overall sensitivity of the latter PHOSPHAN variant. As compared with ITBB monoinfection, the frequency of positive samples in patients with mixed ITBB + HGA infection was significantly higher (p < 0.05). At the disseminated stage of the disease, the proportion of positive reactions with any of Borrelia antigens for IgM was comparable or higher than for C6-IgG that resulted in a substantial number of patients with negative reactions for IgG; this necessitates the use of alternative methods (e.g., PCR) to exclude false-positive serologic results.https://www.epidemvac.ru/jour/article/view/8иксодовые клещевые боррелиозылокализованная и диссеминированная стадии заболеваниямикст-инфекция с гранулоцитарным анаплазмозом человекагуморальный иммунитетмультиплексный иммуночиповый анализ фосфан™пептид с6рекомбинантные белки ospc и vlseixodid tick-borne borrelioses (itbb)localized and disseminated stages of disease with itbbcoinfection with itbb and hgamultiplex microarray immunoassay phosphan<sup>tm</sup>igm and igg to borrelia antigensc6 peptiderecombinant proteins ospc and vlse |
| spellingShingle | V. G. Pomelova E. I. Korenberg T. I. Kuznetsova N. S. Osin The Features of a Humoral Immune Response Development during the Acute Phase of ixodes Tick-Borne Borreliosis (Based on Multiplex Analysis with the Immunochip) Эпидемиология и вакцинопрофилактика иксодовые клещевые боррелиозы локализованная и диссеминированная стадии заболевания микст-инфекция с гранулоцитарным анаплазмозом человека гуморальный иммунитет мультиплексный иммуночиповый анализ фосфан™ пептид с6 рекомбинантные белки ospc и vlse ixodid tick-borne borrelioses (itbb) localized and disseminated stages of disease with itbb coinfection with itbb and hga multiplex microarray immunoassay phosphan<sup>tm</sup> igm and igg to borrelia antigens c6 peptide recombinant proteins ospc and vlse |
| title | The Features of a Humoral Immune Response Development during the Acute Phase of ixodes Tick-Borne Borreliosis (Based on Multiplex Analysis with the Immunochip) |
| title_full | The Features of a Humoral Immune Response Development during the Acute Phase of ixodes Tick-Borne Borreliosis (Based on Multiplex Analysis with the Immunochip) |
| title_fullStr | The Features of a Humoral Immune Response Development during the Acute Phase of ixodes Tick-Borne Borreliosis (Based on Multiplex Analysis with the Immunochip) |
| title_full_unstemmed | The Features of a Humoral Immune Response Development during the Acute Phase of ixodes Tick-Borne Borreliosis (Based on Multiplex Analysis with the Immunochip) |
| title_short | The Features of a Humoral Immune Response Development during the Acute Phase of ixodes Tick-Borne Borreliosis (Based on Multiplex Analysis with the Immunochip) |
| title_sort | features of a humoral immune response development during the acute phase of ixodes tick borne borreliosis based on multiplex analysis with the immunochip |
| topic | иксодовые клещевые боррелиозы локализованная и диссеминированная стадии заболевания микст-инфекция с гранулоцитарным анаплазмозом человека гуморальный иммунитет мультиплексный иммуночиповый анализ фосфан™ пептид с6 рекомбинантные белки ospc и vlse ixodid tick-borne borrelioses (itbb) localized and disseminated stages of disease with itbb coinfection with itbb and hga multiplex microarray immunoassay phosphan<sup>tm</sup> igm and igg to borrelia antigens c6 peptide recombinant proteins ospc and vlse |
| url | https://www.epidemvac.ru/jour/article/view/8 |
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