Design and Manufacture of Bone Cements Based on Calcium Sulfate Hemihydrate and Mg, Sr-Doped Bioactive Glass
In the present study, a novel composite bone cement based on calcium sulfate hemihydrate (CSH) and Mg, Sr-containing bioactive glass (BG) as solid phase, and solution of chitosan as liquid phase were developed. The phase composition, morphology, setting time, injectability, viscosity, and cellular r...
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| Format: | Article |
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MDPI AG
2023-10-01
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| Series: | Biomedicines |
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| Online Access: | https://www.mdpi.com/2227-9059/11/10/2833 |
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| author | Nazanin Moazeni Saeed Hesaraki Aliasghar Behnamghader Javad Esmaeilzadeh Gorka Orive Alireza Dolatshahi-Pirouz Shokoufeh Borhan |
| author_facet | Nazanin Moazeni Saeed Hesaraki Aliasghar Behnamghader Javad Esmaeilzadeh Gorka Orive Alireza Dolatshahi-Pirouz Shokoufeh Borhan |
| author_sort | Nazanin Moazeni |
| collection | DOAJ |
| description | In the present study, a novel composite bone cement based on calcium sulfate hemihydrate (CSH) and Mg, Sr-containing bioactive glass (BG) as solid phase, and solution of chitosan as liquid phase were developed. The phase composition, morphology, setting time, injectability, viscosity, and cellular responses of the composites with various contents of BG (0, 10, 20, and 30 wt.%) were investigated. The pure calcium sulfate cement was set at approximately 180 min, whereas the setting time was drastically decreased to 6 min by replacing 30 wt.% glass powder for CSH in the cement solid phase. BG changed the microscopic morphology of the set cement and decreased the size and compaction of the precipitated gypsum phase. Replacing the CSH phase with BG increased injection force of the produced cement; however, all the cements were injected at a nearly constant force, lower than 20 N. The viscosity measurements in oscillatory mode determined the shear-thinning behavior of the pastes. Although the viscosity of the pastes increased with increasing BG content, it was influenced by the frequency extent. Pure calcium sulfate cement exhibited some transient cytotoxicity on human-derived bone mesenchymal stem cells and it was compensated by introducing BG phase. Moreover, BG improved the cell proliferation and mineralization of extracellular matrix as shown by calcein measurements. The results indicate the injectable composite cement comprising 70 wt.% CSH and 30 wt.% Mg, Sr-doped BG has better setting, mechanical and cellular behaviors and hence, is a potential candidate for bone repair, however more animal and human clinical evaluations are essential. |
| first_indexed | 2024-03-10T21:24:27Z |
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| language | English |
| last_indexed | 2024-03-10T21:24:27Z |
| publishDate | 2023-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomedicines |
| spelling | doaj.art-60984f657bc847fc892c97af196c3dfb2023-11-19T15:47:46ZengMDPI AGBiomedicines2227-90592023-10-011110283310.3390/biomedicines11102833Design and Manufacture of Bone Cements Based on Calcium Sulfate Hemihydrate and Mg, Sr-Doped Bioactive GlassNazanin Moazeni0Saeed Hesaraki1Aliasghar Behnamghader2Javad Esmaeilzadeh3Gorka Orive4Alireza Dolatshahi-Pirouz5Shokoufeh Borhan6Nanotechnology and Advanced Materials Department, Materials and Energy Research Center, Karaj 31779-83634, Alborz, IranNanotechnology and Advanced Materials Department, Materials and Energy Research Center, Karaj 31779-83634, Alborz, IranNanotechnology and Advanced Materials Department, Materials and Energy Research Center, Karaj 31779-83634, Alborz, IranDepartment of Materials and Chemical Engineering, Esfarayen University of Technology, Esfarayen 96619-98195, North Khorasan, IranNanoBioCel Research Group, School of Pharmacy, University of the Basque Country (UPV/EHU), 01006 Vitoria-Gasteiz, SpainDepartment of Health Technology, Technical University of Denmark, 2800 Lyngby, DenmarkDepartment of Materials, Chemical and Polymer Engineering, Buein Zahra Technical University, Buein Zahra 34518-66391, Qazvin, IranIn the present study, a novel composite bone cement based on calcium sulfate hemihydrate (CSH) and Mg, Sr-containing bioactive glass (BG) as solid phase, and solution of chitosan as liquid phase were developed. The phase composition, morphology, setting time, injectability, viscosity, and cellular responses of the composites with various contents of BG (0, 10, 20, and 30 wt.%) were investigated. The pure calcium sulfate cement was set at approximately 180 min, whereas the setting time was drastically decreased to 6 min by replacing 30 wt.% glass powder for CSH in the cement solid phase. BG changed the microscopic morphology of the set cement and decreased the size and compaction of the precipitated gypsum phase. Replacing the CSH phase with BG increased injection force of the produced cement; however, all the cements were injected at a nearly constant force, lower than 20 N. The viscosity measurements in oscillatory mode determined the shear-thinning behavior of the pastes. Although the viscosity of the pastes increased with increasing BG content, it was influenced by the frequency extent. Pure calcium sulfate cement exhibited some transient cytotoxicity on human-derived bone mesenchymal stem cells and it was compensated by introducing BG phase. Moreover, BG improved the cell proliferation and mineralization of extracellular matrix as shown by calcein measurements. The results indicate the injectable composite cement comprising 70 wt.% CSH and 30 wt.% Mg, Sr-doped BG has better setting, mechanical and cellular behaviors and hence, is a potential candidate for bone repair, however more animal and human clinical evaluations are essential.https://www.mdpi.com/2227-9059/11/10/2833calcium sulfatebioactive glassrheologybone cementchitosanSr-doped biomaterials |
| spellingShingle | Nazanin Moazeni Saeed Hesaraki Aliasghar Behnamghader Javad Esmaeilzadeh Gorka Orive Alireza Dolatshahi-Pirouz Shokoufeh Borhan Design and Manufacture of Bone Cements Based on Calcium Sulfate Hemihydrate and Mg, Sr-Doped Bioactive Glass Biomedicines calcium sulfate bioactive glass rheology bone cement chitosan Sr-doped biomaterials |
| title | Design and Manufacture of Bone Cements Based on Calcium Sulfate Hemihydrate and Mg, Sr-Doped Bioactive Glass |
| title_full | Design and Manufacture of Bone Cements Based on Calcium Sulfate Hemihydrate and Mg, Sr-Doped Bioactive Glass |
| title_fullStr | Design and Manufacture of Bone Cements Based on Calcium Sulfate Hemihydrate and Mg, Sr-Doped Bioactive Glass |
| title_full_unstemmed | Design and Manufacture of Bone Cements Based on Calcium Sulfate Hemihydrate and Mg, Sr-Doped Bioactive Glass |
| title_short | Design and Manufacture of Bone Cements Based on Calcium Sulfate Hemihydrate and Mg, Sr-Doped Bioactive Glass |
| title_sort | design and manufacture of bone cements based on calcium sulfate hemihydrate and mg sr doped bioactive glass |
| topic | calcium sulfate bioactive glass rheology bone cement chitosan Sr-doped biomaterials |
| url | https://www.mdpi.com/2227-9059/11/10/2833 |
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