Induction of circulating T follicular helper cells and regulatory T cells correlating with HIV-1 gp120 variable loop antibodies by a subtype C prophylactic vaccine tested in a Phase I trial in India.
A Phase I HIV-1 vaccine trial sponsored by the International AIDS Vaccine Initiative (IAVI) was conducted in India in 2009 to test a subtype C prophylactic vaccine in a prime-boost regimen comprising of a DNA prime (ADVAX) and MVA (TBC-M4) boost. The trial demonstrated that the regimen was safe and...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2018-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC6114930?pdf=render |
| _version_ | 1818360556145344512 |
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| author | Sivasankaran Munusamy Ponnan Soumya Swaminathan Kannan Tiruvengadam Vidyavijayan K K Narayana Cheedarla Manohar Nesakumar Sujitha Kathirvel Rajat Goyal Nikhil Singla Joyeeta Mukherjee Philip Bergin Jakub T Kopycinski Jill Gilmour Srikanth Prasad Tripathy Hanna Elizabeth Luke |
| author_facet | Sivasankaran Munusamy Ponnan Soumya Swaminathan Kannan Tiruvengadam Vidyavijayan K K Narayana Cheedarla Manohar Nesakumar Sujitha Kathirvel Rajat Goyal Nikhil Singla Joyeeta Mukherjee Philip Bergin Jakub T Kopycinski Jill Gilmour Srikanth Prasad Tripathy Hanna Elizabeth Luke |
| author_sort | Sivasankaran Munusamy Ponnan |
| collection | DOAJ |
| description | A Phase I HIV-1 vaccine trial sponsored by the International AIDS Vaccine Initiative (IAVI) was conducted in India in 2009 to test a subtype C prophylactic vaccine in a prime-boost regimen comprising of a DNA prime (ADVAX) and MVA (TBC-M4) boost. The trial demonstrated that the regimen was safe and well tolerated and resulted in enhancement of HIV-specific immune responses. Preliminary observations on vaccine-induced immune responses were limited to analysis of neutralizing antibodies and IFN-γ ELISPOT response. The present study involves a more detailed analysis of the nature of the vaccine-induced humoral immune response using specimens that were archived from the volunteers at the time of the trial. Interestingly, we found vaccine induced production of V1/V2 and V3 region-specific antibodies in a significant proportion of vaccinees. Variable region antibody levels correlated directly with the frequency of circulating T follicular helper cells (Tfh) and regulatory T cells (Treg). Our findings provide encouraging evidence to demonstrate the immunogenicity of the tested vaccine. Better insights into vaccine-induced immune responses can aid in informing future design of a successfulHIV-1 vaccine. |
| first_indexed | 2024-12-13T21:02:40Z |
| format | Article |
| id | doaj.art-609929367d3044ee84fda31ecc615414 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-13T21:02:40Z |
| publishDate | 2018-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-609929367d3044ee84fda31ecc6154142022-12-21T23:31:33ZengPublic Library of Science (PLoS)PLoS ONE1932-62032018-01-01138e020303710.1371/journal.pone.0203037Induction of circulating T follicular helper cells and regulatory T cells correlating with HIV-1 gp120 variable loop antibodies by a subtype C prophylactic vaccine tested in a Phase I trial in India.Sivasankaran Munusamy PonnanSoumya SwaminathanKannan TiruvengadamVidyavijayan K KNarayana CheedarlaManohar NesakumarSujitha KathirvelRajat GoyalNikhil SinglaJoyeeta MukherjeePhilip BerginJakub T KopycinskiJill GilmourSrikanth Prasad TripathyHanna Elizabeth LukeA Phase I HIV-1 vaccine trial sponsored by the International AIDS Vaccine Initiative (IAVI) was conducted in India in 2009 to test a subtype C prophylactic vaccine in a prime-boost regimen comprising of a DNA prime (ADVAX) and MVA (TBC-M4) boost. The trial demonstrated that the regimen was safe and well tolerated and resulted in enhancement of HIV-specific immune responses. Preliminary observations on vaccine-induced immune responses were limited to analysis of neutralizing antibodies and IFN-γ ELISPOT response. The present study involves a more detailed analysis of the nature of the vaccine-induced humoral immune response using specimens that were archived from the volunteers at the time of the trial. Interestingly, we found vaccine induced production of V1/V2 and V3 region-specific antibodies in a significant proportion of vaccinees. Variable region antibody levels correlated directly with the frequency of circulating T follicular helper cells (Tfh) and regulatory T cells (Treg). Our findings provide encouraging evidence to demonstrate the immunogenicity of the tested vaccine. Better insights into vaccine-induced immune responses can aid in informing future design of a successfulHIV-1 vaccine.http://europepmc.org/articles/PMC6114930?pdf=render |
| spellingShingle | Sivasankaran Munusamy Ponnan Soumya Swaminathan Kannan Tiruvengadam Vidyavijayan K K Narayana Cheedarla Manohar Nesakumar Sujitha Kathirvel Rajat Goyal Nikhil Singla Joyeeta Mukherjee Philip Bergin Jakub T Kopycinski Jill Gilmour Srikanth Prasad Tripathy Hanna Elizabeth Luke Induction of circulating T follicular helper cells and regulatory T cells correlating with HIV-1 gp120 variable loop antibodies by a subtype C prophylactic vaccine tested in a Phase I trial in India. PLoS ONE |
| title | Induction of circulating T follicular helper cells and regulatory T cells correlating with HIV-1 gp120 variable loop antibodies by a subtype C prophylactic vaccine tested in a Phase I trial in India. |
| title_full | Induction of circulating T follicular helper cells and regulatory T cells correlating with HIV-1 gp120 variable loop antibodies by a subtype C prophylactic vaccine tested in a Phase I trial in India. |
| title_fullStr | Induction of circulating T follicular helper cells and regulatory T cells correlating with HIV-1 gp120 variable loop antibodies by a subtype C prophylactic vaccine tested in a Phase I trial in India. |
| title_full_unstemmed | Induction of circulating T follicular helper cells and regulatory T cells correlating with HIV-1 gp120 variable loop antibodies by a subtype C prophylactic vaccine tested in a Phase I trial in India. |
| title_short | Induction of circulating T follicular helper cells and regulatory T cells correlating with HIV-1 gp120 variable loop antibodies by a subtype C prophylactic vaccine tested in a Phase I trial in India. |
| title_sort | induction of circulating t follicular helper cells and regulatory t cells correlating with hiv 1 gp120 variable loop antibodies by a subtype c prophylactic vaccine tested in a phase i trial in india |
| url | http://europepmc.org/articles/PMC6114930?pdf=render |
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