Alteration in sperm characteristics, endocrine balance and redox status in rats rendered diabetic by streptozotocin treatment: attenuating role of Loranthus micranthus
Objectives: Loranthus micranthus is widely used in Nigerian folklore treatment of male infertility and diabetes complications. We investigated this claim in rats rendered diabetic by streptozotocin (STZ). Methods: Induction of diabetes mellitus in adult male Wistar rats was by intraperitoneal inject...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2018-01-01
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| Series: | Redox Report |
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| Online Access: | http://dx.doi.org/10.1080/13510002.2018.1540675 |
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| author | Azubuike P. Ebokaiwe Omamuyovwi M. Ijomone Sharon O. Osawe Chukwuma J. Chukwu Chukwunonso E.C.C. Ejike Guolin Zhang Fei Wang |
| author_facet | Azubuike P. Ebokaiwe Omamuyovwi M. Ijomone Sharon O. Osawe Chukwuma J. Chukwu Chukwunonso E.C.C. Ejike Guolin Zhang Fei Wang |
| author_sort | Azubuike P. Ebokaiwe |
| collection | DOAJ |
| description | Objectives: Loranthus micranthus is widely used in Nigerian folklore treatment of male infertility and diabetes complications. We investigated this claim in rats rendered diabetic by streptozotocin (STZ). Methods: Induction of diabetes mellitus in adult male Wistar rats was by intraperitoneal injection of STZ (60 mg/kg). The diabetic rats were thereafter treated orally once/day with 5 mg/kg Gilbenclamide or L. micranthus (100 mg/kg or 200 mg/kg) and monitored for 14 days. Clinical observations, hormonal profile, oxidative stress parameters, glucose metabolism enzymes, histopathological examination, apoptotic marker immunoreactivity and western blotting in testes and sperm parameters were evaluated to examine effects of L. micranthus on STZ-diabetic rats. Results: L. micranthus treatment significantly reduced the blood glucose level (45.9% and 84.7% on the 7th and 14th post-treatment days, respectively); increased antioxidant status, improved microarchitecture of testes, reduced lipid peroxidation and increased BCl-2 protein expression in diabetic rats relative to control. Furthermore, treatment with L. micranthus increased steroidogenic enzymes activities, levels of steroid hormones and improved sperm quality, relative to control. Conclusion: The anti-diabetic and aphrodisiac properties exhibited by L. micranthus could be contingent on its ability to restore a balance to the compromised redox status that characterizes male reproductive dysfunction in diabetes. |
| first_indexed | 2024-12-10T23:17:27Z |
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| id | doaj.art-6099c37be82d488e84ed627cf01bc5e3 |
| institution | Directory Open Access Journal |
| issn | 1351-0002 1743-2928 |
| language | English |
| last_indexed | 2024-12-10T23:17:27Z |
| publishDate | 2018-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Redox Report |
| spelling | doaj.art-6099c37be82d488e84ed627cf01bc5e32022-12-22T01:29:48ZengTaylor & Francis GroupRedox Report1351-00021743-29282018-01-0123119420510.1080/13510002.2018.15406751540675Alteration in sperm characteristics, endocrine balance and redox status in rats rendered diabetic by streptozotocin treatment: attenuating role of Loranthus micranthusAzubuike P. Ebokaiwe0Omamuyovwi M. Ijomone1Sharon O. Osawe2Chukwuma J. Chukwu3Chukwunonso E.C.C. Ejike4Guolin Zhang5Fei Wang6Federal University Ndufu-Alike IkwoFederal University of Technology AkureKolaDaisi UniversityFederal University Ndufu-Alike IkwoFederal University Ndufu-Alike IkwoChengdu Institute of Biology, Chinese Academy of SciencesChengdu Institute of Biology, Chinese Academy of SciencesObjectives: Loranthus micranthus is widely used in Nigerian folklore treatment of male infertility and diabetes complications. We investigated this claim in rats rendered diabetic by streptozotocin (STZ). Methods: Induction of diabetes mellitus in adult male Wistar rats was by intraperitoneal injection of STZ (60 mg/kg). The diabetic rats were thereafter treated orally once/day with 5 mg/kg Gilbenclamide or L. micranthus (100 mg/kg or 200 mg/kg) and monitored for 14 days. Clinical observations, hormonal profile, oxidative stress parameters, glucose metabolism enzymes, histopathological examination, apoptotic marker immunoreactivity and western blotting in testes and sperm parameters were evaluated to examine effects of L. micranthus on STZ-diabetic rats. Results: L. micranthus treatment significantly reduced the blood glucose level (45.9% and 84.7% on the 7th and 14th post-treatment days, respectively); increased antioxidant status, improved microarchitecture of testes, reduced lipid peroxidation and increased BCl-2 protein expression in diabetic rats relative to control. Furthermore, treatment with L. micranthus increased steroidogenic enzymes activities, levels of steroid hormones and improved sperm quality, relative to control. Conclusion: The anti-diabetic and aphrodisiac properties exhibited by L. micranthus could be contingent on its ability to restore a balance to the compromised redox status that characterizes male reproductive dysfunction in diabetes.http://dx.doi.org/10.1080/13510002.2018.1540675Loranthus micranthusspermiogramdiabetesoxidative stressapoptosissteroid hormonesmale rats |
| spellingShingle | Azubuike P. Ebokaiwe Omamuyovwi M. Ijomone Sharon O. Osawe Chukwuma J. Chukwu Chukwunonso E.C.C. Ejike Guolin Zhang Fei Wang Alteration in sperm characteristics, endocrine balance and redox status in rats rendered diabetic by streptozotocin treatment: attenuating role of Loranthus micranthus Redox Report Loranthus micranthus spermiogram diabetes oxidative stress apoptosis steroid hormones male rats |
| title | Alteration in sperm characteristics, endocrine balance and redox status in rats rendered diabetic by streptozotocin treatment: attenuating role of Loranthus micranthus |
| title_full | Alteration in sperm characteristics, endocrine balance and redox status in rats rendered diabetic by streptozotocin treatment: attenuating role of Loranthus micranthus |
| title_fullStr | Alteration in sperm characteristics, endocrine balance and redox status in rats rendered diabetic by streptozotocin treatment: attenuating role of Loranthus micranthus |
| title_full_unstemmed | Alteration in sperm characteristics, endocrine balance and redox status in rats rendered diabetic by streptozotocin treatment: attenuating role of Loranthus micranthus |
| title_short | Alteration in sperm characteristics, endocrine balance and redox status in rats rendered diabetic by streptozotocin treatment: attenuating role of Loranthus micranthus |
| title_sort | alteration in sperm characteristics endocrine balance and redox status in rats rendered diabetic by streptozotocin treatment attenuating role of loranthus micranthus |
| topic | Loranthus micranthus spermiogram diabetes oxidative stress apoptosis steroid hormones male rats |
| url | http://dx.doi.org/10.1080/13510002.2018.1540675 |
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