The effect of different types of storage solutions on saphenous vein endothelial integrity in diabetic patients undergoing coronary artery bypass grafting
Background/Aim. Taking into consideration the justified popularity of total arterial revascularization, the saphenous vein graft (SVG) is still one of the most utilized conduits in coronary artery bypass grafting (CABG). One of the determining factors of this conduit’s durability is its endothelial...
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| Format: | Article |
| Language: | English |
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Military Health Department, Ministry of Defance, Serbia
2023-01-01
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| Series: | Vojnosanitetski Pregled |
| Subjects: | |
| Online Access: | https://doiserbia.nb.rs/img/doi/0042-8450/2023/0042-84502300051K.pdf |
| _version_ | 1827288359382286336 |
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| author | Kamenov Aleksandar Stojiljković Vladimir Petrović Aleksandar Lazarević Milan Golubović Mlađan Perić Velimir Stošić Marija Radenković Goran Nikolić Ivan Petrović Vladimir Živić Saša Milić Dragan |
| author_facet | Kamenov Aleksandar Stojiljković Vladimir Petrović Aleksandar Lazarević Milan Golubović Mlađan Perić Velimir Stošić Marija Radenković Goran Nikolić Ivan Petrović Vladimir Živić Saša Milić Dragan |
| author_sort | Kamenov Aleksandar |
| collection | DOAJ |
| description | Background/Aim. Taking into consideration the justified popularity of total arterial revascularization, the saphenous vein graft (SVG) is still one of the most utilized conduits in coronary artery bypass grafting (CABG). One of the determining factors of this conduit’s durability is its endothelial integrity at the time of surgery. The aim of the study was to investigate the effect of different storage solutions on SVG endothelial integrity in patients with diabetes mellitus (DM) type 2 (T2DM) and non-T2DM patients undergoing CABG. The solutions under evaluation were heparinized saline solution (0.9% NaCl), heparinized autologous whole blood, Bretschneider (histidine-tryptophan-ketoglutarate – HTK) solution, and fresh frozen plasma (FFP) solution. Methods. Forty patients were included in this study and were divided into two groups: group A with 23 T2DM patients and group B with 17 non-T2DM patients. The effects of these solutions were examined by immunohistochemical staining with anti-CD34 antibodies and morphometric comparison in histologic samples of T2DM patients undergoing CABG between July 2021 and September 2022 with samples provided by non-T2DM patients. Results. In this study, the FFP solution showed the most prominent positive effect on the preservation of SVG endothelial integrity, with an average cell integrity preservation of 92.2%. HTK solution was found to be the least effective, with an endothelial cell preservation integrity of 26.77%. There was no marked statistically significant difference in results from groups A and B. There was a noticeable contrast in preserving SVG endothelial integrity between the two patient groups, T2DM and non-T2DM patients, although it was not statistically significant. Conclusion. The storage solution with the most beneficial effect on SVG endothelial integrity preservation was the FFP solution when harvested via the conventional open method in CABG. |
| first_indexed | 2024-04-24T11:30:55Z |
| format | Article |
| id | doaj.art-609a3775addb4939ae147ed7db32a3e2 |
| institution | Directory Open Access Journal |
| issn | 0042-8450 2406-0720 |
| language | English |
| last_indexed | 2024-04-24T11:30:55Z |
| publishDate | 2023-01-01 |
| publisher | Military Health Department, Ministry of Defance, Serbia |
| record_format | Article |
| series | Vojnosanitetski Pregled |
| spelling | doaj.art-609a3775addb4939ae147ed7db32a3e22024-04-10T10:28:41ZengMilitary Health Department, Ministry of Defance, SerbiaVojnosanitetski Pregled0042-84502406-07202023-01-0180121008101510.2298/VSP230312051K0042-84502300051KThe effect of different types of storage solutions on saphenous vein endothelial integrity in diabetic patients undergoing coronary artery bypass graftingKamenov Aleksandar0https://orcid.org/0009-0006-5939-8780Stojiljković Vladimir1Petrović Aleksandar2Lazarević Milan3https://orcid.org/0000-0001-8898-948XGolubović Mlađan4Perić Velimir5Stošić Marija6https://orcid.org/0000-0001-9921-8488Radenković Goran7https://orcid.org/0000-0001-8974-402XNikolić Ivan8Petrović Vladimir9Živić Saša10Milić Dragan11University Clinical Center Niš, Cardiac Surgery Clinic, Niš, SerbiaUniversity Clinical Center Niš, Cardiac Surgery Clinic, Niš, SerbiaUniversity of Niš, Faculty of Medicine, Department of Histology and Embryology, Niš, SerbiaUniversity Clinical Center Niš, Cardiac Surgery Clinic, Niš, SerbiaUniversity Clinical Center Niš, Cardiac Surgery Clinic, Niš, SerbiaUniversity Clinical Center Niš, Cardiac Surgery Clinic, Niš, SerbiaUniversity Clinical Center Niš, Cardiac Surgery Clinic, Niš, SerbiaUniversity of Niš, Faculty of Medicine, Department of Histology and Embryology, Niš, SerbiaUniversity of Niš, Faculty of Medicine, Department of Histology and Embryology, Niš, SerbiaUniversity of Niš, Faculty of Medicine, Department of Histology and Embryology, Niš, SerbiaUniversity Clinical Center Niš, Cardiac Surgery Clinic, Niš, SerbiaUniversity Clinical Center Niš, Cardiac Surgery Clinic, Niš, SerbiaBackground/Aim. Taking into consideration the justified popularity of total arterial revascularization, the saphenous vein graft (SVG) is still one of the most utilized conduits in coronary artery bypass grafting (CABG). One of the determining factors of this conduit’s durability is its endothelial integrity at the time of surgery. The aim of the study was to investigate the effect of different storage solutions on SVG endothelial integrity in patients with diabetes mellitus (DM) type 2 (T2DM) and non-T2DM patients undergoing CABG. The solutions under evaluation were heparinized saline solution (0.9% NaCl), heparinized autologous whole blood, Bretschneider (histidine-tryptophan-ketoglutarate – HTK) solution, and fresh frozen plasma (FFP) solution. Methods. Forty patients were included in this study and were divided into two groups: group A with 23 T2DM patients and group B with 17 non-T2DM patients. The effects of these solutions were examined by immunohistochemical staining with anti-CD34 antibodies and morphometric comparison in histologic samples of T2DM patients undergoing CABG between July 2021 and September 2022 with samples provided by non-T2DM patients. Results. In this study, the FFP solution showed the most prominent positive effect on the preservation of SVG endothelial integrity, with an average cell integrity preservation of 92.2%. HTK solution was found to be the least effective, with an endothelial cell preservation integrity of 26.77%. There was no marked statistically significant difference in results from groups A and B. There was a noticeable contrast in preserving SVG endothelial integrity between the two patient groups, T2DM and non-T2DM patients, although it was not statistically significant. Conclusion. The storage solution with the most beneficial effect on SVG endothelial integrity preservation was the FFP solution when harvested via the conventional open method in CABG.https://doiserbia.nb.rs/img/doi/0042-8450/2023/0042-84502300051K.pdfcoronary artery bypassdiabetes mellitus, type 2histological techniquesorgan preservation solutionssaphenous veintransplants |
| spellingShingle | Kamenov Aleksandar Stojiljković Vladimir Petrović Aleksandar Lazarević Milan Golubović Mlađan Perić Velimir Stošić Marija Radenković Goran Nikolić Ivan Petrović Vladimir Živić Saša Milić Dragan The effect of different types of storage solutions on saphenous vein endothelial integrity in diabetic patients undergoing coronary artery bypass grafting Vojnosanitetski Pregled coronary artery bypass diabetes mellitus, type 2 histological techniques organ preservation solutions saphenous vein transplants |
| title | The effect of different types of storage solutions on saphenous vein endothelial integrity in diabetic patients undergoing coronary artery bypass grafting |
| title_full | The effect of different types of storage solutions on saphenous vein endothelial integrity in diabetic patients undergoing coronary artery bypass grafting |
| title_fullStr | The effect of different types of storage solutions on saphenous vein endothelial integrity in diabetic patients undergoing coronary artery bypass grafting |
| title_full_unstemmed | The effect of different types of storage solutions on saphenous vein endothelial integrity in diabetic patients undergoing coronary artery bypass grafting |
| title_short | The effect of different types of storage solutions on saphenous vein endothelial integrity in diabetic patients undergoing coronary artery bypass grafting |
| title_sort | effect of different types of storage solutions on saphenous vein endothelial integrity in diabetic patients undergoing coronary artery bypass grafting |
| topic | coronary artery bypass diabetes mellitus, type 2 histological techniques organ preservation solutions saphenous vein transplants |
| url | https://doiserbia.nb.rs/img/doi/0042-8450/2023/0042-84502300051K.pdf |
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