Antimicrobial effect of a drinking water additive comprising four organic acids on Campylobacter load in broilers and monitoring of bacterial susceptibility
ABSTRACT: Application of organic acids via feed or drinking water is under discussion as a possible intervention strategy to reduce Campylobacter (C.) load in primary poultry production. A previous in vitro study showed that reduced concentrations of sorbic acid, benzoic acid, propionic acid, and ac...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-12-01
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| Series: | Poultry Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579122005053 |
| _version_ | 1811216756792885248 |
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| author | Vanessa Szott Elisa Peh Anika Friese Uwe Roesler Corinna Kehrenberg Madeleine Ploetz Sophie Kittler |
| author_facet | Vanessa Szott Elisa Peh Anika Friese Uwe Roesler Corinna Kehrenberg Madeleine Ploetz Sophie Kittler |
| author_sort | Vanessa Szott |
| collection | DOAJ |
| description | ABSTRACT: Application of organic acids via feed or drinking water is under discussion as a possible intervention strategy to reduce Campylobacter (C.) load in primary poultry production. A previous in vitro study showed that reduced concentrations of sorbic acid, benzoic acid, propionic acid, and acetic acid were required for antibacterial activity against Campylobacter when using a mixture of these 4 acids compared to when using the single acids. The present study aimed at determining the antibacterial efficiency of this combination in vivo as a drinking water additive for reducing shedding and intestinal C. jejuni colonization in broilers. Furthermore, we assessed whether the inoculated C. jejuni strain BfR-CA-14430 adapted in vivo to the applied organic acids. Results of this study showed that adding the organic acids consistently reduced Campylobacter loads in cloacal swabs. While significant reductions were observed within the entire study period, a maximum 2 log reduction occurred at an age of 18 d. However, after dissection at the end of the trial, no significant differences were detected in Campylobacter loads of cecal and colon contents compared to the control group. Susceptibility testing of re-isolates from cloacal swabs and cecal content revealed equal minimum inhibitory concentration (MIC) values compared to the inoculated test strain, suggesting that C. jejuni remained susceptible throughout the trial. |
| first_indexed | 2024-04-12T06:43:39Z |
| format | Article |
| id | doaj.art-609bcf11c70b453ab99469187bbcb547 |
| institution | Directory Open Access Journal |
| issn | 0032-5791 |
| language | English |
| last_indexed | 2024-04-12T06:43:39Z |
| publishDate | 2022-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj.art-609bcf11c70b453ab99469187bbcb5472022-12-22T03:43:38ZengElsevierPoultry Science0032-57912022-12-0110112102209Antimicrobial effect of a drinking water additive comprising four organic acids on Campylobacter load in broilers and monitoring of bacterial susceptibilityVanessa Szott0Elisa Peh1Anika Friese2Uwe Roesler3Corinna Kehrenberg4Madeleine Ploetz5Sophie Kittler6Institute for Animal Hygiene and Environmental Health, Freie Universität Berlin, Berlin, GermanyInstitute for Food Quality and Food Safety, University of Veterinary Medicine Hannover, Foundation, Hannover, 30173 Germany; Corresponding author:Institute for Animal Hygiene and Environmental Health, Freie Universität Berlin, Berlin, GermanyInstitute for Animal Hygiene and Environmental Health, Freie Universität Berlin, Berlin, GermanyInstitute for Veterinary Food Science, Justus-Liebig-University Giessen, Giessen, GermanyInstitute for Food Quality and Food Safety, University of Veterinary Medicine Hannover, Foundation, Hannover, 30173 GermanyInstitute for Food Quality and Food Safety, University of Veterinary Medicine Hannover, Foundation, Hannover, 30173 GermanyABSTRACT: Application of organic acids via feed or drinking water is under discussion as a possible intervention strategy to reduce Campylobacter (C.) load in primary poultry production. A previous in vitro study showed that reduced concentrations of sorbic acid, benzoic acid, propionic acid, and acetic acid were required for antibacterial activity against Campylobacter when using a mixture of these 4 acids compared to when using the single acids. The present study aimed at determining the antibacterial efficiency of this combination in vivo as a drinking water additive for reducing shedding and intestinal C. jejuni colonization in broilers. Furthermore, we assessed whether the inoculated C. jejuni strain BfR-CA-14430 adapted in vivo to the applied organic acids. Results of this study showed that adding the organic acids consistently reduced Campylobacter loads in cloacal swabs. While significant reductions were observed within the entire study period, a maximum 2 log reduction occurred at an age of 18 d. However, after dissection at the end of the trial, no significant differences were detected in Campylobacter loads of cecal and colon contents compared to the control group. Susceptibility testing of re-isolates from cloacal swabs and cecal content revealed equal minimum inhibitory concentration (MIC) values compared to the inoculated test strain, suggesting that C. jejuni remained susceptible throughout the trial.http://www.sciencedirect.com/science/article/pii/S0032579122005053mitigationcolonizationresistanceadaptationin vivo |
| spellingShingle | Vanessa Szott Elisa Peh Anika Friese Uwe Roesler Corinna Kehrenberg Madeleine Ploetz Sophie Kittler Antimicrobial effect of a drinking water additive comprising four organic acids on Campylobacter load in broilers and monitoring of bacterial susceptibility Poultry Science mitigation colonization resistance adaptation in vivo |
| title | Antimicrobial effect of a drinking water additive comprising four organic acids on Campylobacter load in broilers and monitoring of bacterial susceptibility |
| title_full | Antimicrobial effect of a drinking water additive comprising four organic acids on Campylobacter load in broilers and monitoring of bacterial susceptibility |
| title_fullStr | Antimicrobial effect of a drinking water additive comprising four organic acids on Campylobacter load in broilers and monitoring of bacterial susceptibility |
| title_full_unstemmed | Antimicrobial effect of a drinking water additive comprising four organic acids on Campylobacter load in broilers and monitoring of bacterial susceptibility |
| title_short | Antimicrobial effect of a drinking water additive comprising four organic acids on Campylobacter load in broilers and monitoring of bacterial susceptibility |
| title_sort | antimicrobial effect of a drinking water additive comprising four organic acids on campylobacter load in broilers and monitoring of bacterial susceptibility |
| topic | mitigation colonization resistance adaptation in vivo |
| url | http://www.sciencedirect.com/science/article/pii/S0032579122005053 |
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