miR-22 eluting cardiovascular stent based on a self-healable spongy coating inhibits in-stent restenosis
The in-stent restenosis (IRS) after the percutaneous coronary intervention contributes to the major treatment failure of stent implantation. MicroRNAs have been revealed as powerful gene medicine to regulate endothelial cells (EC) and smooth muscle cells (SMC) in response to vascular injury, providi...
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2021-12-01
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| Series: | Bioactive Materials |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2452199X21002139 |
| _version_ | 1797203230764040192 |
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| author | Jing Wang Hong-Lin Qian Sheng-Yu Chen Wei-Pin Huang Dan-Ni Huang Hong-Ye Hao Ke-Feng Ren Yun-Bing Wang Guo-Sheng Fu Jian Ji |
| author_facet | Jing Wang Hong-Lin Qian Sheng-Yu Chen Wei-Pin Huang Dan-Ni Huang Hong-Ye Hao Ke-Feng Ren Yun-Bing Wang Guo-Sheng Fu Jian Ji |
| author_sort | Jing Wang |
| collection | DOAJ |
| description | The in-stent restenosis (IRS) after the percutaneous coronary intervention contributes to the major treatment failure of stent implantation. MicroRNAs have been revealed as powerful gene medicine to regulate endothelial cells (EC) and smooth muscle cells (SMC) in response to vascular injury, providing a promising therapeutic candidate to inhibit IRS. However, the controllable loading and eluting of hydrophilic bioactive microRNAs pose a challenge to current lipophilic stent coatings. Here, we developed a microRNA eluting cardiovascular stent via the self-healing encapsulation process based on an amphipathic poly(ε-caprolactone)-poly(ethylene glycol)-poly(ε-caprolactone) (PCL-PEG-PCL, PCEC) triblock copolymer spongy network. The miR-22 was used as a model microRNA to regulate SMC. The dynamic porous coating realized the uniform and controllable loading of miR-22, reaching the highest dosage of 133 pmol cm−2. We demonstrated that the sustained release of miR-22 dramatically enhanced the contractile phenotype of SMC without interfering with the proliferation of EC, thus leading to the EC dominating growth at an EC/SMC ratio of 5.4. More importantly, the PCEC@miR-22 coated stents showed reduced inflammation, low switching of SMC phenotype, and low secretion of extracellular matrix, which significantly inhibited IRS. This work provides a simple and robust coating platform for the delivery of microRNAs on cardiovascular stent, which may extend to other combination medical devices, and facilitate practical application of bioactive agents in clinics. |
| first_indexed | 2024-04-24T08:16:02Z |
| format | Article |
| id | doaj.art-609bfb92b34b4677bad82be162f87b3f |
| institution | Directory Open Access Journal |
| issn | 2452-199X |
| language | English |
| last_indexed | 2024-04-24T08:16:02Z |
| publishDate | 2021-12-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Bioactive Materials |
| spelling | doaj.art-609bfb92b34b4677bad82be162f87b3f2024-04-17T03:24:51ZengKeAi Communications Co., Ltd.Bioactive Materials2452-199X2021-12-0161246864696miR-22 eluting cardiovascular stent based on a self-healable spongy coating inhibits in-stent restenosisJing Wang0Hong-Lin Qian1Sheng-Yu Chen2Wei-Pin Huang3Dan-Ni Huang4Hong-Ye Hao5Ke-Feng Ren6Yun-Bing Wang7Guo-Sheng Fu8Jian Ji9MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou, 310027, ChinaMOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou, 310027, ChinaKey Laboratory of Cardiovascular Intervention and Regenerative Medicine of Zhejiang Province, Department of Cardiology, Sir Run Run Shaw Hospital, Zhejiang University, Hangzhou, 310016, ChinaMOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou, 310027, ChinaMOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou, 310027, ChinaMOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou, 310027, ChinaMOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou, 310027, China; Key Laboratory of Cardiovascular Intervention and Regenerative Medicine of Zhejiang Province, Department of Cardiology, Sir Run Run Shaw Hospital, Zhejiang University, Hangzhou, 310016, China; Corresponding author. MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou, 310027, China.National Engineering Research Center for Biomaterials, Sichuan University, Chengdu, 610064, China; Corresponding author.Key Laboratory of Cardiovascular Intervention and Regenerative Medicine of Zhejiang Province, Department of Cardiology, Sir Run Run Shaw Hospital, Zhejiang University, Hangzhou, 310016, China; Corresponding author.MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou, 310027, China; Key Laboratory of Cardiovascular Intervention and Regenerative Medicine of Zhejiang Province, Department of Cardiology, Sir Run Run Shaw Hospital, Zhejiang University, Hangzhou, 310016, ChinaThe in-stent restenosis (IRS) after the percutaneous coronary intervention contributes to the major treatment failure of stent implantation. MicroRNAs have been revealed as powerful gene medicine to regulate endothelial cells (EC) and smooth muscle cells (SMC) in response to vascular injury, providing a promising therapeutic candidate to inhibit IRS. However, the controllable loading and eluting of hydrophilic bioactive microRNAs pose a challenge to current lipophilic stent coatings. Here, we developed a microRNA eluting cardiovascular stent via the self-healing encapsulation process based on an amphipathic poly(ε-caprolactone)-poly(ethylene glycol)-poly(ε-caprolactone) (PCL-PEG-PCL, PCEC) triblock copolymer spongy network. The miR-22 was used as a model microRNA to regulate SMC. The dynamic porous coating realized the uniform and controllable loading of miR-22, reaching the highest dosage of 133 pmol cm−2. We demonstrated that the sustained release of miR-22 dramatically enhanced the contractile phenotype of SMC without interfering with the proliferation of EC, thus leading to the EC dominating growth at an EC/SMC ratio of 5.4. More importantly, the PCEC@miR-22 coated stents showed reduced inflammation, low switching of SMC phenotype, and low secretion of extracellular matrix, which significantly inhibited IRS. This work provides a simple and robust coating platform for the delivery of microRNAs on cardiovascular stent, which may extend to other combination medical devices, and facilitate practical application of bioactive agents in clinics.http://www.sciencedirect.com/science/article/pii/S2452199X21002139microRNASpongy coatingBiodegradable stentContractile phenotypeRestenosis |
| spellingShingle | Jing Wang Hong-Lin Qian Sheng-Yu Chen Wei-Pin Huang Dan-Ni Huang Hong-Ye Hao Ke-Feng Ren Yun-Bing Wang Guo-Sheng Fu Jian Ji miR-22 eluting cardiovascular stent based on a self-healable spongy coating inhibits in-stent restenosis Bioactive Materials microRNA Spongy coating Biodegradable stent Contractile phenotype Restenosis |
| title | miR-22 eluting cardiovascular stent based on a self-healable spongy coating inhibits in-stent restenosis |
| title_full | miR-22 eluting cardiovascular stent based on a self-healable spongy coating inhibits in-stent restenosis |
| title_fullStr | miR-22 eluting cardiovascular stent based on a self-healable spongy coating inhibits in-stent restenosis |
| title_full_unstemmed | miR-22 eluting cardiovascular stent based on a self-healable spongy coating inhibits in-stent restenosis |
| title_short | miR-22 eluting cardiovascular stent based on a self-healable spongy coating inhibits in-stent restenosis |
| title_sort | mir 22 eluting cardiovascular stent based on a self healable spongy coating inhibits in stent restenosis |
| topic | microRNA Spongy coating Biodegradable stent Contractile phenotype Restenosis |
| url | http://www.sciencedirect.com/science/article/pii/S2452199X21002139 |
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