Effects of restraint stress on the regulation of hippocampal glutamate receptor and inflammation genes in female C57BL/6 and BALB/c mice
The two strains of inbred mice, BALB/c and C57BL/6, are widely used in pre-clinical psychiatry research due to their differences in stress susceptibility. Gene profiling studies in these strains have implicated the inflammation pathway as the main contributor to these differences. We focused our att...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2019-02-01
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| Series: | Neurobiology of Stress |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352289519300049 |
| _version_ | 1811214720433127424 |
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| author | Joshua McWhirt Monica Sathyanesan Dayalan Sampath Samuel S. Newton |
| author_facet | Joshua McWhirt Monica Sathyanesan Dayalan Sampath Samuel S. Newton |
| author_sort | Joshua McWhirt |
| collection | DOAJ |
| description | The two strains of inbred mice, BALB/c and C57BL/6, are widely used in pre-clinical psychiatry research due to their differences in stress susceptibility. Gene profiling studies in these strains have implicated the inflammation pathway as the main contributor to these differences. We focused our attention on female mice and tested their response to 5- or 10-day exposure to restraint stress. We examined the stress induced changes in the regulation of 11 inflammatory cytokine genes and 12 glutamate receptor genes in the hippocampus of female BALB/c and C57BL/6 mice using quantitative PCR. Elevated proinflammatory cytokine genes include Tumor Necrosis Factor alpha (TNFa), nuclear factor kappa-light-chain-enhancer of activated B cells (NFKB), Interleukin 1 alpha (IL1a), Interleukin 1 receptor (IL1R), Interleukin 10 receptor alpha subunit (IL10Ra), Interleukin 10 receptor beta subunit (IL10Rb), and tumor necrosis factor (TNF) super family members. Our results show that BALB/c and C57BL/6 mice differ in the genes induced in response to stress exposure and the level of gene regulation change. Our results show that the gene regulation in female BALB/c and C57BL/6 mice differs between strains in the genes regulated and the magnitude of the changes. Keywords: Female, Gene expression, Glutamate receptors, Hippocampus, Proinflammatory genes, Restraint stress |
| first_indexed | 2024-04-12T06:08:13Z |
| format | Article |
| id | doaj.art-609cac17865d4ca187fb04861c39e126 |
| institution | Directory Open Access Journal |
| issn | 2352-2895 |
| language | English |
| last_indexed | 2024-04-12T06:08:13Z |
| publishDate | 2019-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Neurobiology of Stress |
| spelling | doaj.art-609cac17865d4ca187fb04861c39e1262022-12-22T03:44:46ZengElsevierNeurobiology of Stress2352-28952019-02-0110Effects of restraint stress on the regulation of hippocampal glutamate receptor and inflammation genes in female C57BL/6 and BALB/c miceJoshua McWhirt0Monica Sathyanesan1Dayalan Sampath2Samuel S. Newton3Division of Basic Biomedical Sciences, University of South Dakota, Vermillion, SD, 57069, USADivision of Basic Biomedical Sciences, Sanford School of Medicine, University of South Dakota, Vermillion, SD 57069, Sioux Falls VA Healthcare System, Sioux Falls, SD, 57105, USADivision of Basic Biomedical Sciences, Sanford School of Medicine, University of South Dakota, Vermillion, SD, 57069, USADivision of Basic Biomedical Sciences, Sanford School of Medicine, University of South Dakota, Vermillion, SD 57069, Sioux Falls VA Healthcare System, Sioux Falls, SD, 57105, USA; Corresponding author. Division of Basic Biomedical Sciences, Sanford School of Medicine, University of South Dakota, Vermillion, SD 57069; Sioux Falls VA Healthcare System, Sioux Falls, SD, 57105, USA.The two strains of inbred mice, BALB/c and C57BL/6, are widely used in pre-clinical psychiatry research due to their differences in stress susceptibility. Gene profiling studies in these strains have implicated the inflammation pathway as the main contributor to these differences. We focused our attention on female mice and tested their response to 5- or 10-day exposure to restraint stress. We examined the stress induced changes in the regulation of 11 inflammatory cytokine genes and 12 glutamate receptor genes in the hippocampus of female BALB/c and C57BL/6 mice using quantitative PCR. Elevated proinflammatory cytokine genes include Tumor Necrosis Factor alpha (TNFa), nuclear factor kappa-light-chain-enhancer of activated B cells (NFKB), Interleukin 1 alpha (IL1a), Interleukin 1 receptor (IL1R), Interleukin 10 receptor alpha subunit (IL10Ra), Interleukin 10 receptor beta subunit (IL10Rb), and tumor necrosis factor (TNF) super family members. Our results show that BALB/c and C57BL/6 mice differ in the genes induced in response to stress exposure and the level of gene regulation change. Our results show that the gene regulation in female BALB/c and C57BL/6 mice differs between strains in the genes regulated and the magnitude of the changes. Keywords: Female, Gene expression, Glutamate receptors, Hippocampus, Proinflammatory genes, Restraint stresshttp://www.sciencedirect.com/science/article/pii/S2352289519300049 |
| spellingShingle | Joshua McWhirt Monica Sathyanesan Dayalan Sampath Samuel S. Newton Effects of restraint stress on the regulation of hippocampal glutamate receptor and inflammation genes in female C57BL/6 and BALB/c mice Neurobiology of Stress |
| title | Effects of restraint stress on the regulation of hippocampal glutamate receptor and inflammation genes in female C57BL/6 and BALB/c mice |
| title_full | Effects of restraint stress on the regulation of hippocampal glutamate receptor and inflammation genes in female C57BL/6 and BALB/c mice |
| title_fullStr | Effects of restraint stress on the regulation of hippocampal glutamate receptor and inflammation genes in female C57BL/6 and BALB/c mice |
| title_full_unstemmed | Effects of restraint stress on the regulation of hippocampal glutamate receptor and inflammation genes in female C57BL/6 and BALB/c mice |
| title_short | Effects of restraint stress on the regulation of hippocampal glutamate receptor and inflammation genes in female C57BL/6 and BALB/c mice |
| title_sort | effects of restraint stress on the regulation of hippocampal glutamate receptor and inflammation genes in female c57bl 6 and balb c mice |
| url | http://www.sciencedirect.com/science/article/pii/S2352289519300049 |
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