Whole Genome Sequence Analysis of Lactiplantibacillus plantarum Bacteriophage P2
Phage P2 was isolated from failed fermentation broth carried out by Lactiplantibacillus plantarum IMAU10120. A previous study in our laboratory showed that this phage belonged to the Siphoviridae family. In this study, this phage’s genomic characteristics were analyzed using whole-genome sequencing....
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| Format: | Article |
| Language: | English |
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Sciendo
2022-09-01
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| Series: | Polish Journal of Microbiology |
| Subjects: | |
| Online Access: | https://doi.org/10.33073/pjm-2022-037 |
| _version_ | 1828099927604985856 |
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| author | Zhu Hanfang Guo She Zhao Jie Arbab Sakandar Hafiz Lv Ruirui Wen Qiannan Chen Xia |
| author_facet | Zhu Hanfang Guo She Zhao Jie Arbab Sakandar Hafiz Lv Ruirui Wen Qiannan Chen Xia |
| author_sort | Zhu Hanfang |
| collection | DOAJ |
| description | Phage P2 was isolated from failed fermentation broth carried out by Lactiplantibacillus plantarum IMAU10120. A previous study in our laboratory showed that this phage belonged to the Siphoviridae family. In this study, this phage’s genomic characteristics were analyzed using whole-genome sequencing. It was revealed that phage P2 was 77.9 kb in length and had 39.28% G + C content. Its genome included 96 coding sequences (CDS) and two tRNA genes involved in the function of the structure, DNA replication, packaging, and regulation. Phage P2 had higher host specificity; many tested strains were not infected. Cell wall adsorption experiments showed that the adsorption receptor component of phage P2 might be a part of the cell wall peptidoglycan. This research might enrich the knowledge about genomic information of lactobacillus phages and provide some primary data to establish phage control measures. |
| first_indexed | 2024-04-11T08:21:20Z |
| format | Article |
| id | doaj.art-609cbb599cb247e8b859247617d901f8 |
| institution | Directory Open Access Journal |
| issn | 2544-4646 |
| language | English |
| last_indexed | 2024-04-11T08:21:20Z |
| publishDate | 2022-09-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Polish Journal of Microbiology |
| spelling | doaj.art-609cbb599cb247e8b859247617d901f82022-12-22T04:34:57ZengSciendoPolish Journal of Microbiology2544-46462022-09-0171342142810.33073/pjm-2022-037Whole Genome Sequence Analysis of Lactiplantibacillus plantarum Bacteriophage P2Zhu Hanfang0Guo She1Zhao Jie2Arbab Sakandar Hafiz3Lv Ruirui4Wen Qiannan5Chen Xia6Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University, Hohhot, P.R.ChinaKey Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University, Hohhot, P.R.ChinaKey Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University, Hohhot, P.R.ChinaKey Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University, Hohhot, P.R.ChinaKey Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University, Hohhot, P.R.ChinaKey Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University, Hohhot, P.R.ChinaKey Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University, Hohhot, P.R.ChinaPhage P2 was isolated from failed fermentation broth carried out by Lactiplantibacillus plantarum IMAU10120. A previous study in our laboratory showed that this phage belonged to the Siphoviridae family. In this study, this phage’s genomic characteristics were analyzed using whole-genome sequencing. It was revealed that phage P2 was 77.9 kb in length and had 39.28% G + C content. Its genome included 96 coding sequences (CDS) and two tRNA genes involved in the function of the structure, DNA replication, packaging, and regulation. Phage P2 had higher host specificity; many tested strains were not infected. Cell wall adsorption experiments showed that the adsorption receptor component of phage P2 might be a part of the cell wall peptidoglycan. This research might enrich the knowledge about genomic information of lactobacillus phages and provide some primary data to establish phage control measures.https://doi.org/10.33073/pjm-2022-037lactiplantibacillus plantarum phage p2genome characteristicsfunctional geneshost specificityadsorption receptor |
| spellingShingle | Zhu Hanfang Guo She Zhao Jie Arbab Sakandar Hafiz Lv Ruirui Wen Qiannan Chen Xia Whole Genome Sequence Analysis of Lactiplantibacillus plantarum Bacteriophage P2 Polish Journal of Microbiology lactiplantibacillus plantarum phage p2 genome characteristics functional genes host specificity adsorption receptor |
| title | Whole Genome Sequence Analysis of Lactiplantibacillus plantarum Bacteriophage P2 |
| title_full | Whole Genome Sequence Analysis of Lactiplantibacillus plantarum Bacteriophage P2 |
| title_fullStr | Whole Genome Sequence Analysis of Lactiplantibacillus plantarum Bacteriophage P2 |
| title_full_unstemmed | Whole Genome Sequence Analysis of Lactiplantibacillus plantarum Bacteriophage P2 |
| title_short | Whole Genome Sequence Analysis of Lactiplantibacillus plantarum Bacteriophage P2 |
| title_sort | whole genome sequence analysis of lactiplantibacillus plantarum bacteriophage p2 |
| topic | lactiplantibacillus plantarum phage p2 genome characteristics functional genes host specificity adsorption receptor |
| url | https://doi.org/10.33073/pjm-2022-037 |
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