Immobilizing type I collagen via natural cross-linker genipin to enhance the osteogenic responses to titanium implant surface
Titanium (Ti) surface, with an essentially bioinert character, is not easy to actively initiate interactions with surrounding biological tissue. In this study, we developed a novel surface modification to enhance the osteogenic response to Ti surface in orthopedic and dental implant applications. Th...
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| Format: | Article |
| Language: | English |
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Elsevier
2021-11-01
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| Series: | Journal of Materials Research and Technology |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2238785421008838 |
| _version_ | 1818969341977165824 |
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| author | Chia-Fei Liu Kai-Chun Chang Ying-Sui Sun Diem Thuy Nguyen Her-Hsiung Huang |
| author_facet | Chia-Fei Liu Kai-Chun Chang Ying-Sui Sun Diem Thuy Nguyen Her-Hsiung Huang |
| author_sort | Chia-Fei Liu |
| collection | DOAJ |
| description | Titanium (Ti) surface, with an essentially bioinert character, is not easy to actively initiate interactions with surrounding biological tissue. In this study, we developed a novel surface modification to enhance the osteogenic response to Ti surface in orthopedic and dental implant applications. The proposed surface modification combined alkaline treatment and type I collagen immobilization through using a natural cross-linker genipin. A variety of experiments were used to characterize the resulting surfaces in terms of morphology, chemistry, roughness, hydrophilicity, and bioactivity. We also assessed the responses of human bone marrow mesenchymal stem cells (hMSCs) to Ti surfaces in terms of adhesion, migration, proliferation, mineralization, and differentiation. Results showed that the modified Ti surfaces revealed a hydrophilic (water and diiodomethane contact angles <22°) nano-/submicron-scaled porous network porosity with surface roughness Ra ~0.1 μm and good bioactivity (Ca/P formation ability). The collagen-immobilized Ti surfaces with good coating adhesion was shown to facilitate the mineralization of the extracellular matrix and the expressions of specific osteogenic markers (osteopontin, bone sialoprotein, and osteocalcin) in hMSCs, thanks to improved cell adhesion and migration. Overall, the proposed Ti implant surface modification proved highly effective in terms of promoting osteogenic responses in vitro. |
| first_indexed | 2024-12-20T14:19:03Z |
| format | Article |
| id | doaj.art-60a036f5e5f64d0b92dc6d19f6507962 |
| institution | Directory Open Access Journal |
| issn | 2238-7854 |
| language | English |
| last_indexed | 2024-12-20T14:19:03Z |
| publishDate | 2021-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Materials Research and Technology |
| spelling | doaj.art-60a036f5e5f64d0b92dc6d19f65079622022-12-21T19:37:58ZengElsevierJournal of Materials Research and Technology2238-78542021-11-0115885900Immobilizing type I collagen via natural cross-linker genipin to enhance the osteogenic responses to titanium implant surfaceChia-Fei Liu0Kai-Chun Chang1Ying-Sui Sun2Diem Thuy Nguyen3Her-Hsiung Huang4Department of Dentistry, National Yang Ming Chiao Tung University, Taipei, TaiwanInstitute of Oral Biology, National Yang Ming Chiao Tung University, Taipei, TaiwanDepartment of Dentistry, National Yang Ming Chiao Tung University, Taipei, Taiwan; School of Dental Technology, Taipei Medical University, Taipei, TaiwanDepartment of Dentistry, National Yang Ming Chiao Tung University, Taipei, TaiwanDepartment of Dentistry, National Yang Ming Chiao Tung University, Taipei, Taiwan; Institute of Oral Biology, National Yang Ming Chiao Tung University, Taipei, Taiwan; Department of Bioinformatics and Medical Engineering, Asia University, Taichung, Taiwan; Department of Medical Research, China Medical University Hospital, China Medical University, Taichung, Taiwan; Department of Stomatology, Taipei Veterans General Hospital, Taipei, Taiwan; Department of Education and Research, Taipei City Hospital, Taipei, Taiwan; Corresponding author.Titanium (Ti) surface, with an essentially bioinert character, is not easy to actively initiate interactions with surrounding biological tissue. In this study, we developed a novel surface modification to enhance the osteogenic response to Ti surface in orthopedic and dental implant applications. The proposed surface modification combined alkaline treatment and type I collagen immobilization through using a natural cross-linker genipin. A variety of experiments were used to characterize the resulting surfaces in terms of morphology, chemistry, roughness, hydrophilicity, and bioactivity. We also assessed the responses of human bone marrow mesenchymal stem cells (hMSCs) to Ti surfaces in terms of adhesion, migration, proliferation, mineralization, and differentiation. Results showed that the modified Ti surfaces revealed a hydrophilic (water and diiodomethane contact angles <22°) nano-/submicron-scaled porous network porosity with surface roughness Ra ~0.1 μm and good bioactivity (Ca/P formation ability). The collagen-immobilized Ti surfaces with good coating adhesion was shown to facilitate the mineralization of the extracellular matrix and the expressions of specific osteogenic markers (osteopontin, bone sialoprotein, and osteocalcin) in hMSCs, thanks to improved cell adhesion and migration. Overall, the proposed Ti implant surface modification proved highly effective in terms of promoting osteogenic responses in vitro.http://www.sciencedirect.com/science/article/pii/S2238785421008838Osteogenic responseTitanium implantSurface modificationType I collagenGenipinNatural cross-linker |
| spellingShingle | Chia-Fei Liu Kai-Chun Chang Ying-Sui Sun Diem Thuy Nguyen Her-Hsiung Huang Immobilizing type I collagen via natural cross-linker genipin to enhance the osteogenic responses to titanium implant surface Journal of Materials Research and Technology Osteogenic response Titanium implant Surface modification Type I collagen Genipin Natural cross-linker |
| title | Immobilizing type I collagen via natural cross-linker genipin to enhance the osteogenic responses to titanium implant surface |
| title_full | Immobilizing type I collagen via natural cross-linker genipin to enhance the osteogenic responses to titanium implant surface |
| title_fullStr | Immobilizing type I collagen via natural cross-linker genipin to enhance the osteogenic responses to titanium implant surface |
| title_full_unstemmed | Immobilizing type I collagen via natural cross-linker genipin to enhance the osteogenic responses to titanium implant surface |
| title_short | Immobilizing type I collagen via natural cross-linker genipin to enhance the osteogenic responses to titanium implant surface |
| title_sort | immobilizing type i collagen via natural cross linker genipin to enhance the osteogenic responses to titanium implant surface |
| topic | Osteogenic response Titanium implant Surface modification Type I collagen Genipin Natural cross-linker |
| url | http://www.sciencedirect.com/science/article/pii/S2238785421008838 |
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