Direct Somatic Embryogenesis in Commercial Varieties of Corchorus L.: Morphological, Histo-chemical, and Ultrastructural Aspects
Corchorus L. is an eminent source of industrial fibers. To understand the regeneration capacity, somatic embryogenesis (SEG) has been investigated. In this study, leaf explants are used for induction of the direct somatic embryos (DSEs). Light and scanning electron microscopy (SEM) has been implemen...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2022-10-01
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| Series: | Journal of Natural Fibers |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/15440478.2020.1848739 |
| _version_ | 1797680097318141952 |
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| author | Raju Mondal Kanti Meena Asit B Mandal |
| author_facet | Raju Mondal Kanti Meena Asit B Mandal |
| author_sort | Raju Mondal |
| collection | DOAJ |
| description | Corchorus L. is an eminent source of industrial fibers. To understand the regeneration capacity, somatic embryogenesis (SEG) has been investigated. In this study, leaf explants are used for induction of the direct somatic embryos (DSEs). Light and scanning electron microscopy (SEM) has been implemented to confirm the DSEs. The influences of genotypes and plant growth regulators (PGRs) on the SEG have also been evaluated. Among the four studied verities, var. JRC 517 has shown a maximum response aftre one month of dark condition in 0.5 mg l−1IAA with 0.03 mg l−1 kin fortified basal medium. The germination rate of the DSEs is obtained about 54.8% in 1 mg l−1 GA3 fortified media. The subsequent rate of plantlets conversion has achieved about 31.8% in ½ MS media. The presence of polysaccharide and protein is observed as reserved material upon histochemical study. The unicellular origin of DSE has been confirmed by the cyto-histochemical study. Abbreviations: 2,4-D, 2,4-dichlorophenoxyacetic acid; DAPI, 4′-6-diamidino-2-phenylindole; DSE, direct somatic embryos; GA3, gibberellic acid; IAA, indole-3-acetic acid; MS medium, Murashige and Skoog medium; NBB, naphthol blue-black; PAS, periodic acid-Schiff; PGR, plant growth regulators; PCA, principal component analysis; SEG, somatic embryogenesis; SEM, scanning electron microscopy |
| first_indexed | 2024-03-11T23:24:45Z |
| format | Article |
| id | doaj.art-60a0b54c78a9492abdc3beaff76ba067 |
| institution | Directory Open Access Journal |
| issn | 1544-0478 1544-046X |
| language | English |
| last_indexed | 2024-03-11T23:24:45Z |
| publishDate | 2022-10-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of Natural Fibers |
| spelling | doaj.art-60a0b54c78a9492abdc3beaff76ba0672023-09-20T13:04:25ZengTaylor & Francis GroupJournal of Natural Fibers1544-04781544-046X2022-10-0119103954396310.1080/15440478.2020.18487391848739Direct Somatic Embryogenesis in Commercial Varieties of Corchorus L.: Morphological, Histo-chemical, and Ultrastructural AspectsRaju Mondal0Kanti Meena1Asit B Mandal2Central Sericultural Germplasm Resources Centre (CSGRC)ICAR-Central Research Institute For Jute And Allied FibresICAR-Central Research Institute For Jute And Allied FibresCorchorus L. is an eminent source of industrial fibers. To understand the regeneration capacity, somatic embryogenesis (SEG) has been investigated. In this study, leaf explants are used for induction of the direct somatic embryos (DSEs). Light and scanning electron microscopy (SEM) has been implemented to confirm the DSEs. The influences of genotypes and plant growth regulators (PGRs) on the SEG have also been evaluated. Among the four studied verities, var. JRC 517 has shown a maximum response aftre one month of dark condition in 0.5 mg l−1IAA with 0.03 mg l−1 kin fortified basal medium. The germination rate of the DSEs is obtained about 54.8% in 1 mg l−1 GA3 fortified media. The subsequent rate of plantlets conversion has achieved about 31.8% in ½ MS media. The presence of polysaccharide and protein is observed as reserved material upon histochemical study. The unicellular origin of DSE has been confirmed by the cyto-histochemical study. Abbreviations: 2,4-D, 2,4-dichlorophenoxyacetic acid; DAPI, 4′-6-diamidino-2-phenylindole; DSE, direct somatic embryos; GA3, gibberellic acid; IAA, indole-3-acetic acid; MS medium, Murashige and Skoog medium; NBB, naphthol blue-black; PAS, periodic acid-Schiff; PGR, plant growth regulators; PCA, principal component analysis; SEG, somatic embryogenesis; SEM, scanning electron microscopyhttp://dx.doi.org/10.1080/15440478.2020.1848739jrc 517embryo inductionconversionprincipal component analysis (pca) |
| spellingShingle | Raju Mondal Kanti Meena Asit B Mandal Direct Somatic Embryogenesis in Commercial Varieties of Corchorus L.: Morphological, Histo-chemical, and Ultrastructural Aspects Journal of Natural Fibers jrc 517 embryo induction conversion principal component analysis (pca) |
| title | Direct Somatic Embryogenesis in Commercial Varieties of Corchorus L.: Morphological, Histo-chemical, and Ultrastructural Aspects |
| title_full | Direct Somatic Embryogenesis in Commercial Varieties of Corchorus L.: Morphological, Histo-chemical, and Ultrastructural Aspects |
| title_fullStr | Direct Somatic Embryogenesis in Commercial Varieties of Corchorus L.: Morphological, Histo-chemical, and Ultrastructural Aspects |
| title_full_unstemmed | Direct Somatic Embryogenesis in Commercial Varieties of Corchorus L.: Morphological, Histo-chemical, and Ultrastructural Aspects |
| title_short | Direct Somatic Embryogenesis in Commercial Varieties of Corchorus L.: Morphological, Histo-chemical, and Ultrastructural Aspects |
| title_sort | direct somatic embryogenesis in commercial varieties of corchorus l morphological histo chemical and ultrastructural aspects |
| topic | jrc 517 embryo induction conversion principal component analysis (pca) |
| url | http://dx.doi.org/10.1080/15440478.2020.1848739 |
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