Morphological and Molecular Identification of Jujube Fruit Fly Carpomya incompleta (Becker, 1903) (Diptera: Tephritidae) in southeast Iraq

An accurate identification of an insect is essential for the effective pest management program. However, due to the restrictions of phenotypic identification, molecular process (e.g. DNA barcoding) is used to overcome some of these limitations. The present paper was aimed to identify the Jujube frui...

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Main Authors: Huda M. Taher, Aqeel Alyousuf
Format: Article
Language:English
Published: University of Basrah 2023-06-01
Series:Maǧallaẗ al-baṣraẗ al-ʻulūm al-zirāʻiyyaẗ
Subjects:
Online Access:https://bjas.bajas.edu.iq/index.php/bjas/article/view/905
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author Huda M. Taher
Aqeel Alyousuf
author_facet Huda M. Taher
Aqeel Alyousuf
author_sort Huda M. Taher
collection DOAJ
description An accurate identification of an insect is essential for the effective pest management program. However, due to the restrictions of phenotypic identification, molecular process (e.g. DNA barcoding) is used to overcome some of these limitations. The present paper was aimed to identify the Jujube fruit fly by morphological characters of the adults and molecular DNA barcoding of the larval stage in southeast Iraq. The adults was morphologically identified as Carpomyia incompleta (Becker, 1903) (Diptera: Tephritidae). Results of the molecular method revealed that the mitochondrial COI – COII marker was successfully identified immature stage of the species. The sequence analysis results identified the specimens (AHL1 and AHL2) as C. incompleta. Sequences of the specimens were recorded in The US National Center for Biotechnology Information (NCBI) under accession numbers (ON045002.1 and ON045003.1, respectively. These results were consistent with the phenotypic identification of the Jujube fruit flies.
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spelling doaj.art-60a2b733f8ee467eaf6a59b1552d33d52023-07-15T17:36:18ZengUniversity of BasrahMaǧallaẗ al-baṣraẗ al-ʻulūm al-zirāʻiyyaẗ1814-58682520-08602023-06-01361Morphological and Molecular Identification of Jujube Fruit Fly Carpomya incompleta (Becker, 1903) (Diptera: Tephritidae) in southeast IraqHuda M. Taher 0Aqeel Alyousuf1Department of Plant Protection, College of Agriculture, University of Basrah, IraqDepartment of Plant Protection, College of Agriculture, University of Basrah, IraqAn accurate identification of an insect is essential for the effective pest management program. However, due to the restrictions of phenotypic identification, molecular process (e.g. DNA barcoding) is used to overcome some of these limitations. The present paper was aimed to identify the Jujube fruit fly by morphological characters of the adults and molecular DNA barcoding of the larval stage in southeast Iraq. The adults was morphologically identified as Carpomyia incompleta (Becker, 1903) (Diptera: Tephritidae). Results of the molecular method revealed that the mitochondrial COI – COII marker was successfully identified immature stage of the species. The sequence analysis results identified the specimens (AHL1 and AHL2) as C. incompleta. Sequences of the specimens were recorded in The US National Center for Biotechnology Information (NCBI) under accession numbers (ON045002.1 and ON045003.1, respectively. These results were consistent with the phenotypic identification of the Jujube fruit flies. https://bjas.bajas.edu.iq/index.php/bjas/article/view/905Carpomya incompletaFruit fliesIdentificationJujubeZizaphus spp.
spellingShingle Huda M. Taher
Aqeel Alyousuf
Morphological and Molecular Identification of Jujube Fruit Fly Carpomya incompleta (Becker, 1903) (Diptera: Tephritidae) in southeast Iraq
Maǧallaẗ al-baṣraẗ al-ʻulūm al-zirāʻiyyaẗ
Carpomya incompleta
Fruit flies
Identification
Jujube
Zizaphus spp.
title Morphological and Molecular Identification of Jujube Fruit Fly Carpomya incompleta (Becker, 1903) (Diptera: Tephritidae) in southeast Iraq
title_full Morphological and Molecular Identification of Jujube Fruit Fly Carpomya incompleta (Becker, 1903) (Diptera: Tephritidae) in southeast Iraq
title_fullStr Morphological and Molecular Identification of Jujube Fruit Fly Carpomya incompleta (Becker, 1903) (Diptera: Tephritidae) in southeast Iraq
title_full_unstemmed Morphological and Molecular Identification of Jujube Fruit Fly Carpomya incompleta (Becker, 1903) (Diptera: Tephritidae) in southeast Iraq
title_short Morphological and Molecular Identification of Jujube Fruit Fly Carpomya incompleta (Becker, 1903) (Diptera: Tephritidae) in southeast Iraq
title_sort morphological and molecular identification of jujube fruit fly carpomya incompleta becker 1903 diptera tephritidae in southeast iraq
topic Carpomya incompleta
Fruit flies
Identification
Jujube
Zizaphus spp.
url https://bjas.bajas.edu.iq/index.php/bjas/article/view/905
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