Mu-DNA: a modular universal DNA extraction method adaptable for a wide range of sample types
Efficient DNA extraction is fundamental to molecular studies. However, commercial kits are expensive when a large number of samples need to be processed. Here we present a simple, modular and adaptable DNA extraction ‘toolkit’ for the isolation of high purity DNA from multiple sample types (modular...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Pensoft Publishers
2018-07-01
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| Series: | Metabarcoding and Metagenomics |
| Online Access: | https://mbmg.pensoft.net/article/24556/ |
| _version_ | 1818792491521933312 |
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| author | Graham S. Sellers Cristina Di Muri Africa Gómez Bernd Hänfling |
| author_facet | Graham S. Sellers Cristina Di Muri Africa Gómez Bernd Hänfling |
| author_sort | Graham S. Sellers |
| collection | DOAJ |
| description | Efficient DNA extraction is fundamental to molecular studies. However, commercial kits are expensive when a large number of samples need to be processed. Here we present a simple, modular and adaptable DNA extraction ‘toolkit’ for the isolation of high purity DNA from multiple sample types (modular universal DNA extraction method or Mu-DNA). We compare the performance of our method to that of widely used commercial kits across a range of soil, stool, tissue and water samples. Mu-DNA produced DNA extractions of similar or higher yield and purity to that of the commercial kits. As a proof of principle, we carried out replicate fish metabarcoding of aquatic eDNA extractions, which confirmed that the species detection efficiency of our method is similar to that of the most frequently used commercial kit. Our results demonstrate the reliability of Mu-DNA along with its modular adaptability to challenging sample types and sample collection methods. Mu-DNA can substantially reduce the costs and increase the scope of experiments in molecular studies. |
| first_indexed | 2024-12-18T15:28:06Z |
| format | Article |
| id | doaj.art-60ad1db0443043618fccfb698c99ba1d |
| institution | Directory Open Access Journal |
| issn | 2534-9708 |
| language | English |
| last_indexed | 2024-12-18T15:28:06Z |
| publishDate | 2018-07-01 |
| publisher | Pensoft Publishers |
| record_format | Article |
| series | Metabarcoding and Metagenomics |
| spelling | doaj.art-60ad1db0443043618fccfb698c99ba1d2022-12-21T21:03:13ZengPensoft PublishersMetabarcoding and Metagenomics2534-97082018-07-01211110.3897/mbmg.2.2455624556Mu-DNA: a modular universal DNA extraction method adaptable for a wide range of sample typesGraham S. Sellers0Cristina Di Muri1Africa Gómez2Bernd Hänfling3University of HullUniversity of HullUniversity of HullUniversity of HullEfficient DNA extraction is fundamental to molecular studies. However, commercial kits are expensive when a large number of samples need to be processed. Here we present a simple, modular and adaptable DNA extraction ‘toolkit’ for the isolation of high purity DNA from multiple sample types (modular universal DNA extraction method or Mu-DNA). We compare the performance of our method to that of widely used commercial kits across a range of soil, stool, tissue and water samples. Mu-DNA produced DNA extractions of similar or higher yield and purity to that of the commercial kits. As a proof of principle, we carried out replicate fish metabarcoding of aquatic eDNA extractions, which confirmed that the species detection efficiency of our method is similar to that of the most frequently used commercial kit. Our results demonstrate the reliability of Mu-DNA along with its modular adaptability to challenging sample types and sample collection methods. Mu-DNA can substantially reduce the costs and increase the scope of experiments in molecular studies.https://mbmg.pensoft.net/article/24556/ |
| spellingShingle | Graham S. Sellers Cristina Di Muri Africa Gómez Bernd Hänfling Mu-DNA: a modular universal DNA extraction method adaptable for a wide range of sample types Metabarcoding and Metagenomics |
| title | Mu-DNA: a modular universal DNA extraction method adaptable for a wide range of sample types |
| title_full | Mu-DNA: a modular universal DNA extraction method adaptable for a wide range of sample types |
| title_fullStr | Mu-DNA: a modular universal DNA extraction method adaptable for a wide range of sample types |
| title_full_unstemmed | Mu-DNA: a modular universal DNA extraction method adaptable for a wide range of sample types |
| title_short | Mu-DNA: a modular universal DNA extraction method adaptable for a wide range of sample types |
| title_sort | mu dna a modular universal dna extraction method adaptable for a wide range of sample types |
| url | https://mbmg.pensoft.net/article/24556/ |
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