and feed supplements improve growth performance and gut mucosal architecture with modulations on cecal microbiota in red-feathered native chickens
Objective The aim of study was to investigate the effects of in-feed supplementation of Bacillus amyloliquefaciens (BA) and Saccharomyces cerevisiae (SC) on growth performance, gut integrity, and microbiota modulations in red-feathered native chickens (RFCs). Methods A total of 18,000 RFCs in a comm...
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| Format: | Article |
| Language: | English |
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Asian-Australasian Association of Animal Production Societies
2022-06-01
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| Series: | Animal Bioscience |
| Subjects: | |
| Online Access: | http://www.animbiosci.org/upload/pdf/ab-21-0318.pdf |
| _version_ | 1811292662589816832 |
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| author | Tzu-Tai Lee Chung-Hsi Chou Chinling Wang Hsuan-Ying Lu Wen-Yuan Yang |
| author_facet | Tzu-Tai Lee Chung-Hsi Chou Chinling Wang Hsuan-Ying Lu Wen-Yuan Yang |
| author_sort | Tzu-Tai Lee |
| collection | DOAJ |
| description | Objective The aim of study was to investigate the effects of in-feed supplementation of Bacillus amyloliquefaciens (BA) and Saccharomyces cerevisiae (SC) on growth performance, gut integrity, and microbiota modulations in red-feathered native chickens (RFCs). Methods A total of 18,000 RFCs in a commercial farm were evenly assigned into two dietary treatments (control diet; 0.05% BA and 0.05% SC) by randomization and raised for 11 weeks in two separate houses. Fifty RFCs in each group were randomly selected and raised in the original house with the partition for performance evaluations at the age of 9 and 11 weeks. Six non-partitioned RFCs per group were randomly selected for analyses of intestinal architecture and 16S rRNA metagenomics. Results Feeding BA and SC increased the body weight and body weight gain, significantly at the age of 11 weeks (p<0.05). The villus height/crypt ratio in the small intestines and Firmicutes to Bacteroidetes ratio were also notably increased (p<0.05). The supplementation did not disturb the microbial community structure but promote the featured microbial shifts characterized by the significant increments of Bernesiella, Prevotellaceae_NK3B31_group, and Butyrucimonas, following remarkable decrements of Bacteroides, Rikenellaceae_RC9_gut_group, and Succinatimonas in RFCs with growth benefits. Besides, functional pathways of peptidoglycan biosynthesis, nucleotide excision repair, glycolysis/gluconeogenesis, and aminoacyl transfer ribonucleic acid (tRNA) biosynthesis were significantly promoted (p< 0.05). Conclusion In-feed supplementation of BA and SC enhanced the growth performance, improved mucosal architectures in small intestines, and modulated the cecal microbiota and metabolic pathways in RFCs. |
| first_indexed | 2024-04-13T04:48:42Z |
| format | Article |
| id | doaj.art-60aee4fc6042426eae884526d3cbe5ad |
| institution | Directory Open Access Journal |
| issn | 2765-0189 2765-0235 |
| language | English |
| last_indexed | 2024-04-13T04:48:42Z |
| publishDate | 2022-06-01 |
| publisher | Asian-Australasian Association of Animal Production Societies |
| record_format | Article |
| series | Animal Bioscience |
| spelling | doaj.art-60aee4fc6042426eae884526d3cbe5ad2022-12-22T03:01:44ZengAsian-Australasian Association of Animal Production SocietiesAnimal Bioscience2765-01892765-02352022-06-0135686988310.5713/ab.21.031824800and feed supplements improve growth performance and gut mucosal architecture with modulations on cecal microbiota in red-feathered native chickensTzu-Tai Lee0Chung-Hsi Chou1Chinling Wang2Hsuan-Ying Lu3Wen-Yuan Yang4 Department of Animal Science, National Chung Hsing University, Taichung, 402, Taiwan Department of Veterinary Medicine, School of Veterinary Medicine, National Taiwan University, Taipei City 106, Taiwan Department of Basic Sciences, College of Veterinary Medicine, Mississippi State University, P.O. Box 6100, Mississippi State, MS 39762, USA Department of Veterinary Medicine, School of Veterinary Medicine, National Taiwan University, Taipei City 106, Taiwan Department of Veterinary Medicine, School of Veterinary Medicine, National Taiwan University, Taipei City 106, TaiwanObjective The aim of study was to investigate the effects of in-feed supplementation of Bacillus amyloliquefaciens (BA) and Saccharomyces cerevisiae (SC) on growth performance, gut integrity, and microbiota modulations in red-feathered native chickens (RFCs). Methods A total of 18,000 RFCs in a commercial farm were evenly assigned into two dietary treatments (control diet; 0.05% BA and 0.05% SC) by randomization and raised for 11 weeks in two separate houses. Fifty RFCs in each group were randomly selected and raised in the original house with the partition for performance evaluations at the age of 9 and 11 weeks. Six non-partitioned RFCs per group were randomly selected for analyses of intestinal architecture and 16S rRNA metagenomics. Results Feeding BA and SC increased the body weight and body weight gain, significantly at the age of 11 weeks (p<0.05). The villus height/crypt ratio in the small intestines and Firmicutes to Bacteroidetes ratio were also notably increased (p<0.05). The supplementation did not disturb the microbial community structure but promote the featured microbial shifts characterized by the significant increments of Bernesiella, Prevotellaceae_NK3B31_group, and Butyrucimonas, following remarkable decrements of Bacteroides, Rikenellaceae_RC9_gut_group, and Succinatimonas in RFCs with growth benefits. Besides, functional pathways of peptidoglycan biosynthesis, nucleotide excision repair, glycolysis/gluconeogenesis, and aminoacyl transfer ribonucleic acid (tRNA) biosynthesis were significantly promoted (p< 0.05). Conclusion In-feed supplementation of BA and SC enhanced the growth performance, improved mucosal architectures in small intestines, and modulated the cecal microbiota and metabolic pathways in RFCs.http://www.animbiosci.org/upload/pdf/ab-21-0318.pdfgrowth performancemetagenomicsmicrobiota modulation |
| spellingShingle | Tzu-Tai Lee Chung-Hsi Chou Chinling Wang Hsuan-Ying Lu Wen-Yuan Yang and feed supplements improve growth performance and gut mucosal architecture with modulations on cecal microbiota in red-feathered native chickens Animal Bioscience growth performance metagenomics microbiota modulation |
| title | and feed supplements improve growth performance and gut mucosal architecture with modulations on cecal microbiota in red-feathered native chickens |
| title_full | and feed supplements improve growth performance and gut mucosal architecture with modulations on cecal microbiota in red-feathered native chickens |
| title_fullStr | and feed supplements improve growth performance and gut mucosal architecture with modulations on cecal microbiota in red-feathered native chickens |
| title_full_unstemmed | and feed supplements improve growth performance and gut mucosal architecture with modulations on cecal microbiota in red-feathered native chickens |
| title_short | and feed supplements improve growth performance and gut mucosal architecture with modulations on cecal microbiota in red-feathered native chickens |
| title_sort | and feed supplements improve growth performance and gut mucosal architecture with modulations on cecal microbiota in red feathered native chickens |
| topic | growth performance metagenomics microbiota modulation |
| url | http://www.animbiosci.org/upload/pdf/ab-21-0318.pdf |
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