BMP4 aggravates mitochondrial dysfunction of HRMECs
Mitochondria are important places for the oxidative phosphorylation of glucose and the maintenance of cell oxidation and antioxidant stability. However, mitochondrial dysfunction causes cell dysfunction. Meanwhile, retinal vascular endothelial cell dysfunction may cause vascular inflammation, hemorr...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-03-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844023010319 |
| _version_ | 1797851913113305088 |
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| author | Yong Wang Hui Li Jingjing Cao Aihua Liu Zhenyu Kou Weiting An Jingli Liang Xiaomin Zhang Xiaorong Li Lijie Dong |
| author_facet | Yong Wang Hui Li Jingjing Cao Aihua Liu Zhenyu Kou Weiting An Jingli Liang Xiaomin Zhang Xiaorong Li Lijie Dong |
| author_sort | Yong Wang |
| collection | DOAJ |
| description | Mitochondria are important places for the oxidative phosphorylation of glucose and the maintenance of cell oxidation and antioxidant stability. However, mitochondrial dysfunction causes cell dysfunction. Meanwhile, retinal vascular endothelial cell dysfunction may cause vascular inflammation, hemorrhage, angiogenesis, and other manifestations. Our previous studies have shown that Bone morphogenetic protein 4 (BMP4) is an important target for the treatment of retinal neovascularization, but the mechanism remains unclear. Therefore, our study aims to observe the effects of BMP4 on vascular endothelial cells and hopes to provide a new target for diabetic retinopathy. 4-Hydroxynonenal (4HNE), a kind of lipid peroxide, was used to induce the oxidative stress model. Human retinal microvascular endothelial cells (HRMECs) were randomly divided into control, 4HNE, negative control, and siBMP4 groups. Si-BMP4 significantly reduced leukocyte adhesion and 4HNE-induced high ROS level and restored the mitochondrial membrane potential and OCR. This indicates that BMP4 plays an important role in inducing leukocyte adhesion, oxidative stress, and mitochondrial dysfunction. The relationship between BMP4 and retinal vascular endothelial cell dysfunction is preliminarily confirmed by this study. Mitochondrial dysfunction and oxidative stress may be involved in BMP4-mediated retinal vascular endothelial cell dysfunction. |
| first_indexed | 2024-04-09T19:25:32Z |
| format | Article |
| id | doaj.art-60afec7e3c554c6b90664691cec31e39 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-04-09T19:25:32Z |
| publishDate | 2023-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-60afec7e3c554c6b90664691cec31e392023-04-05T08:17:05ZengElsevierHeliyon2405-84402023-03-0193e13824BMP4 aggravates mitochondrial dysfunction of HRMECsYong Wang0Hui Li1Jingjing Cao2Aihua Liu3Zhenyu Kou4Weiting An5Jingli Liang6Xiaomin Zhang7Xiaorong Li8Lijie Dong9Tianjin Key Laboratory of Retinal Functions and Diseases, PR China; Tianjin Branch of National Clinical Research Center for Ocular Disease, PR China; Eye Institute and School of Optometry, PR China; Tianjin Medical University Eye Hospital, PR ChinaTianjin Key Laboratory of Retinal Functions and Diseases, PR China; Tianjin Branch of National Clinical Research Center for Ocular Disease, PR China; Eye Institute and School of Optometry, PR China; Tianjin Medical University Eye Hospital, PR ChinaTianjin Key Laboratory of Retinal Functions and Diseases, PR China; Tianjin Branch of National Clinical Research Center for Ocular Disease, PR China; Eye Institute and School of Optometry, PR China; Tianjin Medical University Eye Hospital, PR ChinaTianjin Key Laboratory of Retinal Functions and Diseases, PR China; Tianjin Branch of National Clinical Research Center for Ocular Disease, PR China; Eye Institute and School of Optometry, PR China; Tianjin Medical University Eye Hospital, PR ChinaTianjin Key Laboratory of Retinal Functions and Diseases, PR China; Tianjin Branch of National Clinical Research Center for Ocular Disease, PR China; Eye Institute and School of Optometry, PR China; Tianjin Medical University Eye Hospital, PR ChinaTianjin Key Laboratory of Retinal Functions and Diseases, PR China; Tianjin Branch of National Clinical Research Center for Ocular Disease, PR China; Eye Institute and School of Optometry, PR China; Tianjin Medical University Eye Hospital, PR ChinaTianjin Key Laboratory of Retinal Functions and Diseases, PR China; Tianjin Branch of National Clinical Research Center for Ocular Disease, PR China; Eye Institute and School of Optometry, PR China; Tianjin Medical University Eye Hospital, PR ChinaTianjin Key Laboratory of Retinal Functions and Diseases, PR China; Tianjin Branch of National Clinical Research Center for Ocular Disease, PR China; Eye Institute and School of Optometry, PR China; Tianjin Medical University Eye Hospital, PR ChinaTianjin Key Laboratory of Retinal Functions and Diseases, PR China; Tianjin Branch of National Clinical Research Center for Ocular Disease, PR China; Eye Institute and School of Optometry, PR China; Tianjin Medical University Eye Hospital, PR ChinaTianjin Key Laboratory of Retinal Functions and Diseases, PR China; Tianjin Branch of National Clinical Research Center for Ocular Disease, PR China; Eye Institute and School of Optometry, PR China; Tianjin Medical University Eye Hospital, PR China; Corresponding author. Tianjin Medical University Eye Hospital, Tianjin Medical University Eye Institute, 251 Fukang Road, Nankai, Tianjin 300384, PR China.Mitochondria are important places for the oxidative phosphorylation of glucose and the maintenance of cell oxidation and antioxidant stability. However, mitochondrial dysfunction causes cell dysfunction. Meanwhile, retinal vascular endothelial cell dysfunction may cause vascular inflammation, hemorrhage, angiogenesis, and other manifestations. Our previous studies have shown that Bone morphogenetic protein 4 (BMP4) is an important target for the treatment of retinal neovascularization, but the mechanism remains unclear. Therefore, our study aims to observe the effects of BMP4 on vascular endothelial cells and hopes to provide a new target for diabetic retinopathy. 4-Hydroxynonenal (4HNE), a kind of lipid peroxide, was used to induce the oxidative stress model. Human retinal microvascular endothelial cells (HRMECs) were randomly divided into control, 4HNE, negative control, and siBMP4 groups. Si-BMP4 significantly reduced leukocyte adhesion and 4HNE-induced high ROS level and restored the mitochondrial membrane potential and OCR. This indicates that BMP4 plays an important role in inducing leukocyte adhesion, oxidative stress, and mitochondrial dysfunction. The relationship between BMP4 and retinal vascular endothelial cell dysfunction is preliminarily confirmed by this study. Mitochondrial dysfunction and oxidative stress may be involved in BMP4-mediated retinal vascular endothelial cell dysfunction.http://www.sciencedirect.com/science/article/pii/S2405844023010319BMP4Reactive oxygen speciesMitochondrion |
| spellingShingle | Yong Wang Hui Li Jingjing Cao Aihua Liu Zhenyu Kou Weiting An Jingli Liang Xiaomin Zhang Xiaorong Li Lijie Dong BMP4 aggravates mitochondrial dysfunction of HRMECs Heliyon BMP4 Reactive oxygen species Mitochondrion |
| title | BMP4 aggravates mitochondrial dysfunction of HRMECs |
| title_full | BMP4 aggravates mitochondrial dysfunction of HRMECs |
| title_fullStr | BMP4 aggravates mitochondrial dysfunction of HRMECs |
| title_full_unstemmed | BMP4 aggravates mitochondrial dysfunction of HRMECs |
| title_short | BMP4 aggravates mitochondrial dysfunction of HRMECs |
| title_sort | bmp4 aggravates mitochondrial dysfunction of hrmecs |
| topic | BMP4 Reactive oxygen species Mitochondrion |
| url | http://www.sciencedirect.com/science/article/pii/S2405844023010319 |
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