Protocol for the assessment of human T cell activation by real-time metabolic flux analysis

Protocol for the assessment of human T cell activation by real-time metabolic flux analysis

Summary: The elevation of glycolysis in autoreactive T cells is a key target for the prevention and treatment of T cell-related autoimmune diseases, such as type 1 diabetes (T1D). Here, we describe a simple and efficient protocol for isolating human peripheral blood mononuclear cells (PBMCs) and T c...

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Bibliographic Details
Main Authors: Byung Soo Kong, Changhan Lee, Young Min Cho
Format: Article
Language:English
Published: Elsevier 2022-03-01
Series:STAR Protocols
Subjects:
Classification Description: Cell Biology
Cell isolation
Cell-based Assays
Immunology
Metabolism
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721007905

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