Immune response in the larva of the dung beetle Phyllognathus excavatus against human blood cells as foreign bodies
The dung beetle Phyllognathus excavatus (Coleoptera: Scarabaeidae) is a widespread and common species throughout its distribution (North Africa, southern Europe, and Asia). The larvae remain most of their life in compost-rich soil feeding on organic matter. This report aimed to identify and describe...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-06-01
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| Series: | Journal of King Saud University: Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1018364722001288 |
| _version_ | 1811256154005700608 |
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| author | Ramzi Amari Fatma Guesmi Manel Ben Ali Amor Hedfi Issam Saidi Akram S. Alghamdi Bander Albogami Mohamed Sghaier Achouri Mohamed Salah Allagui |
| author_facet | Ramzi Amari Fatma Guesmi Manel Ben Ali Amor Hedfi Issam Saidi Akram S. Alghamdi Bander Albogami Mohamed Sghaier Achouri Mohamed Salah Allagui |
| author_sort | Ramzi Amari |
| collection | DOAJ |
| description | The dung beetle Phyllognathus excavatus (Coleoptera: Scarabaeidae) is a widespread and common species throughout its distribution (North Africa, southern Europe, and Asia). The larvae remain most of their life in compost-rich soil feeding on organic matter. This report aimed to identify and describe the larval immune effector cells (hemocytes) types and to investigate their immunological responses. For this, hemolymph was collected to investigate the different circulating cells using both light and scanning electron microscopy. The Larval hemolymph cells were identified and hemocytes immune responses under-reacting with human blood cells was investigated. The hemogram parameters were analyzed in the larval hemolymph, mixture of larval hemolymph and human blood, and healthy adult man blood (as a reference). Experiments showed that granulocytes, plasmatocytes, and coagulocytes were involved in the first line in the lysis of human red blood cells. Results showed that the larvae present also a great number of platelets-like and lymphocytes-like. Besides, the majority of the human red blood cells were killed under the larval immune defense. Thus, the content of human red blood cells was reduced by 75 % when mixed with the larval hemolymph whose student test showed a significant decrease (α < 0.01). Our findings, validated through immunological responses induced by larvae hemocytes towards human bood cells, show that hemocytes from scarab larvae, involved in immune defense mechanisms, can defend against infections by foreign pathogens and offer an insight into the novel application for mammal diseases. |
| first_indexed | 2024-04-12T17:36:40Z |
| format | Article |
| id | doaj.art-60b22921bb264f86abb0869faee33aed |
| institution | Directory Open Access Journal |
| issn | 1018-3647 |
| language | English |
| last_indexed | 2024-04-12T17:36:40Z |
| publishDate | 2022-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of King Saud University: Science |
| spelling | doaj.art-60b22921bb264f86abb0869faee33aed2022-12-22T03:22:58ZengElsevierJournal of King Saud University: Science1018-36472022-06-01344101947Immune response in the larva of the dung beetle Phyllognathus excavatus against human blood cells as foreign bodiesRamzi Amari0Fatma Guesmi1Manel Ben Ali2Amor Hedfi3Issam Saidi4Akram S. Alghamdi5Bander Albogami6Mohamed Sghaier Achouri7Mohamed Salah Allagui8Laboratory of Biotechnology and Biomonitoring of the Environment and Oasis Ecosystems (LR21ES26), Faculty of Sciences of Gafsa, 2112, Tunisia; Laboratory of Bio-ecology and Evolutionary Systematics, Unit UR 11 ES 11, Faculty of Sciences of Tunis, University of Tunis El Manar, Tunisia; Laboratory of Ecophysiology, Unit UR/13/ES-73, Faculty of Sciences of Sfax, University of Sfax, TunisiaLaboratory of Biotechnology and Biomonitoring of the Environment and Oasis Ecosystems (LR21ES26), Faculty of Sciences of Gafsa, 2112, Tunisia; Laboratory of Risks Related to Environmental Stresses: Fight and Prevention, Unit UR03ES06, Faculty of Sciences of Bizerte, University of Carthage, Tunisia; Corresponding authors.Department of Biology, College of Sciences, Taif University, P.O. Box 11099, Taif 21944, Saudi ArabiaDepartment of Biology, College of Sciences, Taif University, P.O. Box 11099, Taif 21944, Saudi ArabiaLaboratory of Biotechnology and Biomonitoring of the Environment and Oasis Ecosystems (LR21ES26), Faculty of Sciences of Gafsa, 2112, TunisiaDepartment of Biology, College of Sciences, Taif University, P.O. Box 11099, Taif 21944, Saudi ArabiaDepartment of Biology, College of Sciences, Taif University, P.O. Box 11099, Taif 21944, Saudi ArabiaLaboratory of Bio-ecology and Evolutionary Systematics, Unit UR 11 ES 11, Faculty of Sciences of Tunis, University of Tunis El Manar, TunisiaLaboratory of Biotechnology and Biomonitoring of the Environment and Oasis Ecosystems (LR21ES26), Faculty of Sciences of Gafsa, 2112, Tunisia; Laboratory of Bio-ecology and Evolutionary Systematics, Unit UR 11 ES 11, Faculty of Sciences of Tunis, University of Tunis El Manar, Tunisia; Laboratory of Ecophysiology, Unit UR/13/ES-73, Faculty of Sciences of Sfax, University of Sfax, Tunisia; Corresponding authors.The dung beetle Phyllognathus excavatus (Coleoptera: Scarabaeidae) is a widespread and common species throughout its distribution (North Africa, southern Europe, and Asia). The larvae remain most of their life in compost-rich soil feeding on organic matter. This report aimed to identify and describe the larval immune effector cells (hemocytes) types and to investigate their immunological responses. For this, hemolymph was collected to investigate the different circulating cells using both light and scanning electron microscopy. The Larval hemolymph cells were identified and hemocytes immune responses under-reacting with human blood cells was investigated. The hemogram parameters were analyzed in the larval hemolymph, mixture of larval hemolymph and human blood, and healthy adult man blood (as a reference). Experiments showed that granulocytes, plasmatocytes, and coagulocytes were involved in the first line in the lysis of human red blood cells. Results showed that the larvae present also a great number of platelets-like and lymphocytes-like. Besides, the majority of the human red blood cells were killed under the larval immune defense. Thus, the content of human red blood cells was reduced by 75 % when mixed with the larval hemolymph whose student test showed a significant decrease (α < 0.01). Our findings, validated through immunological responses induced by larvae hemocytes towards human bood cells, show that hemocytes from scarab larvae, involved in immune defense mechanisms, can defend against infections by foreign pathogens and offer an insight into the novel application for mammal diseases.http://www.sciencedirect.com/science/article/pii/S1018364722001288Phyllognathus excavatus larvaeHemolymph cellsImmune defenseCoagulocytesGranulocytesPlasmatocytes |
| spellingShingle | Ramzi Amari Fatma Guesmi Manel Ben Ali Amor Hedfi Issam Saidi Akram S. Alghamdi Bander Albogami Mohamed Sghaier Achouri Mohamed Salah Allagui Immune response in the larva of the dung beetle Phyllognathus excavatus against human blood cells as foreign bodies Journal of King Saud University: Science Phyllognathus excavatus larvae Hemolymph cells Immune defense Coagulocytes Granulocytes Plasmatocytes |
| title | Immune response in the larva of the dung beetle Phyllognathus excavatus against human blood cells as foreign bodies |
| title_full | Immune response in the larva of the dung beetle Phyllognathus excavatus against human blood cells as foreign bodies |
| title_fullStr | Immune response in the larva of the dung beetle Phyllognathus excavatus against human blood cells as foreign bodies |
| title_full_unstemmed | Immune response in the larva of the dung beetle Phyllognathus excavatus against human blood cells as foreign bodies |
| title_short | Immune response in the larva of the dung beetle Phyllognathus excavatus against human blood cells as foreign bodies |
| title_sort | immune response in the larva of the dung beetle phyllognathus excavatus against human blood cells as foreign bodies |
| topic | Phyllognathus excavatus larvae Hemolymph cells Immune defense Coagulocytes Granulocytes Plasmatocytes |
| url | http://www.sciencedirect.com/science/article/pii/S1018364722001288 |
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