Prophylactic use of Ganoderma lucidum extract may inhibit Mycobacterium tuberculosis replication in a new mouse model of spontaneous latent tuberculosis infection
A mouse model of spontaneous latent tuberculosis infection (LTBI)that mimics latent tuberculosis infection in humans is valuable for drug/vaccine development and the study of tuberculosis. However, most LTBI mouse models require interventions, and a spontaneous LTBI mouse model with a low bacterial...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2016-01-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.01490/full |
| _version_ | 1818439781394153472 |
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| author | Chuan eQin Zhan eLingjun tang ejun lin eshuzhu xu eyanfeng xu eyuhuan |
| author_facet | Chuan eQin Zhan eLingjun tang ejun lin eshuzhu xu eyanfeng xu eyuhuan |
| author_sort | Chuan eQin |
| collection | DOAJ |
| description | A mouse model of spontaneous latent tuberculosis infection (LTBI)that mimics latent tuberculosis infection in humans is valuable for drug/vaccine development and the study of tuberculosis. However, most LTBI mouse models require interventions, and a spontaneous LTBI mouse model with a low bacterial load is difficult to establish. In this study, mice were IV-inoculated with 100 CFU Mycobacterium tuberculosis H37Rv, and a persistent LTBI was established with low bacterial loads (0.5~1.5log10 CFU in the lung; <4log10 CFU in the spleen). Histopathological changes in the lung, spleen, and liver were mild during the first 20 weeks post-inoculation. The model was used to demonstrate the comparative effects of prophylactic and therapeutic administration of Ganoderma lucidum extract (spores and spores lipid) in preventing H37Rv replication in both lung and spleen. H37Rv was inhibited with prophylactic use of G. lucidum extract relative to that of the untreated control and therapy groups, and observed in the spleen as early as post-inoculation week 3. H37Rv infection in the therapy group was comparable to that of the untreated control mice. No significant mitigation of pathological changes was observed in either the prophylactic or therapeutic groups. Our results suggest that this LTBI model is an efficient means of testing anti-tuberculosis vaccines and drugs. The use of G. lucidum extract prior to M. tuberculosis infection may protect the host against bacterial replication to some extent. |
| first_indexed | 2024-12-14T18:01:55Z |
| format | Article |
| id | doaj.art-60b3e316417d4717b017d47d8406120a |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-14T18:01:55Z |
| publishDate | 2016-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-60b3e316417d4717b017d47d8406120a2022-12-21T22:52:27ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2016-01-01610.3389/fmicb.2015.01490151788Prophylactic use of Ganoderma lucidum extract may inhibit Mycobacterium tuberculosis replication in a new mouse model of spontaneous latent tuberculosis infectionChuan eQin0Zhan eLingjun1tang ejun2lin eshuzhu3xu eyanfeng4xu eyuhuan5Insitute of Laboratory Animal Science, Chinese Academy of Medical Sciences (CAMS)Insitute of Laboratory Animal Science, Chinese Academy of Medical Sciences (CAMS)Insitute of Laboratory Animal Science, Chinese Academy of Medical Sciences (CAMS)Insitute of Laboratory Animal Science, Chinese Academy of Medical Sciences (CAMS)Insitute of Laboratory Animal Science, Chinese Academy of Medical Sciences (CAMS)Insitute of Laboratory Animal Science, Chinese Academy of Medical Sciences (CAMS)A mouse model of spontaneous latent tuberculosis infection (LTBI)that mimics latent tuberculosis infection in humans is valuable for drug/vaccine development and the study of tuberculosis. However, most LTBI mouse models require interventions, and a spontaneous LTBI mouse model with a low bacterial load is difficult to establish. In this study, mice were IV-inoculated with 100 CFU Mycobacterium tuberculosis H37Rv, and a persistent LTBI was established with low bacterial loads (0.5~1.5log10 CFU in the lung; <4log10 CFU in the spleen). Histopathological changes in the lung, spleen, and liver were mild during the first 20 weeks post-inoculation. The model was used to demonstrate the comparative effects of prophylactic and therapeutic administration of Ganoderma lucidum extract (spores and spores lipid) in preventing H37Rv replication in both lung and spleen. H37Rv was inhibited with prophylactic use of G. lucidum extract relative to that of the untreated control and therapy groups, and observed in the spleen as early as post-inoculation week 3. H37Rv infection in the therapy group was comparable to that of the untreated control mice. No significant mitigation of pathological changes was observed in either the prophylactic or therapeutic groups. Our results suggest that this LTBI model is an efficient means of testing anti-tuberculosis vaccines and drugs. The use of G. lucidum extract prior to M. tuberculosis infection may protect the host against bacterial replication to some extent.http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.01490/fullmouse modelGanoderma lucidum extractspontaneous latent tuberculosis infectioninhibited Mycobacterium tuberculosis replicationProphylactic use |
| spellingShingle | Chuan eQin Zhan eLingjun tang ejun lin eshuzhu xu eyanfeng xu eyuhuan Prophylactic use of Ganoderma lucidum extract may inhibit Mycobacterium tuberculosis replication in a new mouse model of spontaneous latent tuberculosis infection Frontiers in Microbiology mouse model Ganoderma lucidum extract spontaneous latent tuberculosis infection inhibited Mycobacterium tuberculosis replication Prophylactic use |
| title | Prophylactic use of Ganoderma lucidum extract may inhibit Mycobacterium tuberculosis replication in a new mouse model of spontaneous latent tuberculosis infection |
| title_full | Prophylactic use of Ganoderma lucidum extract may inhibit Mycobacterium tuberculosis replication in a new mouse model of spontaneous latent tuberculosis infection |
| title_fullStr | Prophylactic use of Ganoderma lucidum extract may inhibit Mycobacterium tuberculosis replication in a new mouse model of spontaneous latent tuberculosis infection |
| title_full_unstemmed | Prophylactic use of Ganoderma lucidum extract may inhibit Mycobacterium tuberculosis replication in a new mouse model of spontaneous latent tuberculosis infection |
| title_short | Prophylactic use of Ganoderma lucidum extract may inhibit Mycobacterium tuberculosis replication in a new mouse model of spontaneous latent tuberculosis infection |
| title_sort | prophylactic use of ganoderma lucidum extract may inhibit mycobacterium tuberculosis replication in a new mouse model of spontaneous latent tuberculosis infection |
| topic | mouse model Ganoderma lucidum extract spontaneous latent tuberculosis infection inhibited Mycobacterium tuberculosis replication Prophylactic use |
| url | http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.01490/full |
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