Does ergothioneine and thawing temperatures improve rooster semen post-thawed quality?
ABSTRACT: The present study focuses on the effect of different levels of ergothioneine and thawing temperature on rooster semen cryopreservation. Semen was diluted in Lake extender containing ergothioneine at 5, 10, 15, and 20 µM and cryopreserved. Two thawing temperatures (37°C for 30 s and 60°C fo...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2021-10-01
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| Series: | Poultry Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579121004284 |
| _version_ | 1818912796438429696 |
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| author | Sahar Abdalkarim Salih Hossein Daghigh-Kia Mahdieh Mehdipour Abouzar Najafi |
| author_facet | Sahar Abdalkarim Salih Hossein Daghigh-Kia Mahdieh Mehdipour Abouzar Najafi |
| author_sort | Sahar Abdalkarim Salih |
| collection | DOAJ |
| description | ABSTRACT: The present study focuses on the effect of different levels of ergothioneine and thawing temperature on rooster semen cryopreservation. Semen was diluted in Lake extender containing ergothioneine at 5, 10, 15, and 20 µM and cryopreserved. Two thawing temperatures (37°C for 30 s and 60°C for 5 s) were consequently examined. Sperm motility parameter, membrane integrity, abnormal morphology, viability, apoptotic status, mitochondria activity, and lipid peroxidation were determined after freeze-thaw process. Ergothioneine levels of 5 and 10 µM led to higher (P < 0.05) total motility (66.58 ± 1.44 and 72.11±1.44, respectively) and average path velocity (VAP) (34.54 ± 0.89, 37.28 ± 0.89, respectively). Higher (P < 0.05) significant membrane integrity and mitochondria activity after freeze-thawing were observed in the groups supplemented with 10 µM ergothioneine (68.62 ± 1.24 and 69.12 ± 1.26, respectively). Also, 5 and 10 µM of ergothioneine led to the lowest significant percentage of apoptotic and dead sperm. The total motility and progressive motility resulted in significantly (P < 0.05) higher amount when sperm were thawed with 60°C (60.58 ± 0.91 and 24.76 ± 0.53, respectively) compared to thawed sperm in 37°C. The membrane integrity, viability and mitochondria activity led to significantly (P < 0.05) higher when sperm were thawed with 60°C (58.2 ± 0.78, 63.21 ± 0.80 and 56.85 ± 0.79, respectively). It could be concluded the addition of 5 and 10 µM ergothioneine in the semen extender and thawing temperature at 60˚C in 5 s can be an efficient strategy to preserve rooster cryopreserved semen quality. |
| first_indexed | 2024-12-19T23:20:17Z |
| format | Article |
| id | doaj.art-60b432cd8a16460cbfb8b4690de8b634 |
| institution | Directory Open Access Journal |
| issn | 0032-5791 |
| language | English |
| last_indexed | 2024-12-19T23:20:17Z |
| publishDate | 2021-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj.art-60b432cd8a16460cbfb8b4690de8b6342022-12-21T20:02:00ZengElsevierPoultry Science0032-57912021-10-0110010101405Does ergothioneine and thawing temperatures improve rooster semen post-thawed quality?Sahar Abdalkarim Salih0Hossein Daghigh-Kia1Mahdieh Mehdipour2Abouzar Najafi3Department of Animal Science, College of Agriculture, University of Tabriz, Tabriz, IranDepartment of Animal Science, College of Agriculture, University of Tabriz, Tabriz, Iran; Corresponding author:Department of Animal Science, College of Agriculture, University of Tabriz, Tabriz, IranDepartment of Animal and Poultry Science, College of Aburaihan, University of Tehran, Tehran, IranABSTRACT: The present study focuses on the effect of different levels of ergothioneine and thawing temperature on rooster semen cryopreservation. Semen was diluted in Lake extender containing ergothioneine at 5, 10, 15, and 20 µM and cryopreserved. Two thawing temperatures (37°C for 30 s and 60°C for 5 s) were consequently examined. Sperm motility parameter, membrane integrity, abnormal morphology, viability, apoptotic status, mitochondria activity, and lipid peroxidation were determined after freeze-thaw process. Ergothioneine levels of 5 and 10 µM led to higher (P < 0.05) total motility (66.58 ± 1.44 and 72.11±1.44, respectively) and average path velocity (VAP) (34.54 ± 0.89, 37.28 ± 0.89, respectively). Higher (P < 0.05) significant membrane integrity and mitochondria activity after freeze-thawing were observed in the groups supplemented with 10 µM ergothioneine (68.62 ± 1.24 and 69.12 ± 1.26, respectively). Also, 5 and 10 µM of ergothioneine led to the lowest significant percentage of apoptotic and dead sperm. The total motility and progressive motility resulted in significantly (P < 0.05) higher amount when sperm were thawed with 60°C (60.58 ± 0.91 and 24.76 ± 0.53, respectively) compared to thawed sperm in 37°C. The membrane integrity, viability and mitochondria activity led to significantly (P < 0.05) higher when sperm were thawed with 60°C (58.2 ± 0.78, 63.21 ± 0.80 and 56.85 ± 0.79, respectively). It could be concluded the addition of 5 and 10 µM ergothioneine in the semen extender and thawing temperature at 60˚C in 5 s can be an efficient strategy to preserve rooster cryopreserved semen quality.http://www.sciencedirect.com/science/article/pii/S0032579121004284roosterergothioneinethawing temperaturecryopreservationsperm |
| spellingShingle | Sahar Abdalkarim Salih Hossein Daghigh-Kia Mahdieh Mehdipour Abouzar Najafi Does ergothioneine and thawing temperatures improve rooster semen post-thawed quality? Poultry Science rooster ergothioneine thawing temperature cryopreservation sperm |
| title | Does ergothioneine and thawing temperatures improve rooster semen post-thawed quality? |
| title_full | Does ergothioneine and thawing temperatures improve rooster semen post-thawed quality? |
| title_fullStr | Does ergothioneine and thawing temperatures improve rooster semen post-thawed quality? |
| title_full_unstemmed | Does ergothioneine and thawing temperatures improve rooster semen post-thawed quality? |
| title_short | Does ergothioneine and thawing temperatures improve rooster semen post-thawed quality? |
| title_sort | does ergothioneine and thawing temperatures improve rooster semen post thawed quality |
| topic | rooster ergothioneine thawing temperature cryopreservation sperm |
| url | http://www.sciencedirect.com/science/article/pii/S0032579121004284 |
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