Tomosyn expression pattern in the mouse hippocampus suggests both presynaptic and postsynaptic functions
The protein tomosyn decreases synaptic transmission and release probability of vesicles, and is essential for modulating synaptic transmission in neurons. In this study, we provide a detailed description of the expression and localization patterns of tomosyn1 and tomosyn2 in the subareas of the mous...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2010-12-01
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| Series: | Frontiers in Neuroanatomy |
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| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fnana.2010.00149/full |
| _version_ | 1818551585823784960 |
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| author | Boaz eBarak Antionette eWilliams Noa eBielopolski Irit eGottfried Eitan eOkun Meghan A Brown Ulf eMatti Jens eRettig Edward L Stuenkel Uri eAshery |
| author_facet | Boaz eBarak Antionette eWilliams Noa eBielopolski Irit eGottfried Eitan eOkun Meghan A Brown Ulf eMatti Jens eRettig Edward L Stuenkel Uri eAshery |
| author_sort | Boaz eBarak |
| collection | DOAJ |
| description | The protein tomosyn decreases synaptic transmission and release probability of vesicles, and is essential for modulating synaptic transmission in neurons. In this study, we provide a detailed description of the expression and localization patterns of tomosyn1 and tomosyn2 in the subareas of the mouse hippocampus. Using confocal and two-photon high-resolution microscopy, we show tomosyn1's differential distribution in the mouse hippocampus, concentrated mainly in the hilus and mossy fibers. Tomosyn colocalizes with several pre- and postsynaptic markers and is found mainly in glutamatergic synapses. Surprisingly, we found that tomosyn2 is expressed in the subiculum, CA1 and CA2 pyramidal cell bodies, dendrites and spines, and colocalizes with PSD95, suggesting a postsynaptic role. These results suggest that in addition to the well-characterized presynaptic function of tomosyn in neurotransmitter release, tomosyn2 might have a postsynaptic function, and place tomosyn as a more general regulator of synaptic transmission and plasticity. |
| first_indexed | 2024-12-12T09:01:52Z |
| format | Article |
| id | doaj.art-60b66e395454475384d58ec2a597ed15 |
| institution | Directory Open Access Journal |
| issn | 1662-5129 |
| language | English |
| last_indexed | 2024-12-12T09:01:52Z |
| publishDate | 2010-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Neuroanatomy |
| spelling | doaj.art-60b66e395454475384d58ec2a597ed152022-12-22T00:29:48ZengFrontiers Media S.A.Frontiers in Neuroanatomy1662-51292010-12-01410.3389/fnana.2010.001497844Tomosyn expression pattern in the mouse hippocampus suggests both presynaptic and postsynaptic functionsBoaz eBarak0Antionette eWilliams1Noa eBielopolski2Irit eGottfried3Eitan eOkun4Meghan A Brown5Ulf eMatti6Jens eRettig7Edward L Stuenkel8Uri eAshery9Tel Aviv UniversityUniversity of MichiganTel Aviv UniversityTel Aviv UniversityNational Institutes of HealthNational Institutes of HealthUniversität des SaarlandesUniversität des SaarlandesUniversity of MichiganTel Aviv UniversityThe protein tomosyn decreases synaptic transmission and release probability of vesicles, and is essential for modulating synaptic transmission in neurons. In this study, we provide a detailed description of the expression and localization patterns of tomosyn1 and tomosyn2 in the subareas of the mouse hippocampus. Using confocal and two-photon high-resolution microscopy, we show tomosyn1's differential distribution in the mouse hippocampus, concentrated mainly in the hilus and mossy fibers. Tomosyn colocalizes with several pre- and postsynaptic markers and is found mainly in glutamatergic synapses. Surprisingly, we found that tomosyn2 is expressed in the subiculum, CA1 and CA2 pyramidal cell bodies, dendrites and spines, and colocalizes with PSD95, suggesting a postsynaptic role. These results suggest that in addition to the well-characterized presynaptic function of tomosyn in neurotransmitter release, tomosyn2 might have a postsynaptic function, and place tomosyn as a more general regulator of synaptic transmission and plasticity.http://journal.frontiersin.org/Journal/10.3389/fnana.2010.00149/fullHippocampusCA2 areamossy fibers terminalssynaptic proteintomosyn isoforms |
| spellingShingle | Boaz eBarak Antionette eWilliams Noa eBielopolski Irit eGottfried Eitan eOkun Meghan A Brown Ulf eMatti Jens eRettig Edward L Stuenkel Uri eAshery Tomosyn expression pattern in the mouse hippocampus suggests both presynaptic and postsynaptic functions Frontiers in Neuroanatomy Hippocampus CA2 area mossy fibers terminals synaptic protein tomosyn isoforms |
| title | Tomosyn expression pattern in the mouse hippocampus suggests both presynaptic and postsynaptic functions |
| title_full | Tomosyn expression pattern in the mouse hippocampus suggests both presynaptic and postsynaptic functions |
| title_fullStr | Tomosyn expression pattern in the mouse hippocampus suggests both presynaptic and postsynaptic functions |
| title_full_unstemmed | Tomosyn expression pattern in the mouse hippocampus suggests both presynaptic and postsynaptic functions |
| title_short | Tomosyn expression pattern in the mouse hippocampus suggests both presynaptic and postsynaptic functions |
| title_sort | tomosyn expression pattern in the mouse hippocampus suggests both presynaptic and postsynaptic functions |
| topic | Hippocampus CA2 area mossy fibers terminals synaptic protein tomosyn isoforms |
| url | http://journal.frontiersin.org/Journal/10.3389/fnana.2010.00149/full |
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