The expression profile of exosomal miRNAs in cervical cancer cells (HeLa) compared to normal cells and evaluating the key genes related to them
Introduction: Cervical cancer is the fourth most common cancer among women worldwide and is a heterogeneous disease. The exosomal miRNAs could be applied as diagnostic biomarkers and therapeutic targets in cervical cancer. The present study was performed with aim to use a bioinformatics analysis to...
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| Format: | Article |
| Language: | fas |
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Mashhad University of Medical Sciences
2023-10-01
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| Series: | Majallah-i Zanān, Māmā̓ī va Nāzā̓ī-i Īrān |
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| Online Access: | https://ijogi.mums.ac.ir/article_23399_f0510515c385c5ccd742fc8ce1d0829b.pdf |
| _version_ | 1797401819237842944 |
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| author | Marzieh Naderi Boldaji Kambiz Ahmadi Somayeh Reiisi |
| author_facet | Marzieh Naderi Boldaji Kambiz Ahmadi Somayeh Reiisi |
| author_sort | Marzieh Naderi Boldaji |
| collection | DOAJ |
| description | Introduction: Cervical cancer is the fourth most common cancer among women worldwide and is a heterogeneous disease. The exosomal miRNAs could be applied as diagnostic biomarkers and therapeutic targets in cervical cancer. The present study was performed with aim to use a bioinformatics analysis to identify key miRNAs and related genes and pathways in exosomes derived from cervical cancer cells.
Methods: In this study, the differential expression of exosomal miRNA and RNAs in healthy and HeLa cells was investigated by analyzing the GEO database for miR-seq and RNA-seq in R software. Subsequently, overlapped miRNA target genes and differentially mRNA from exosome were selected and then, GO and KEGG pathway analysis was performed. The overlapped genes were integrated to construct a PPI network and hub genes selection.
Results: In the present study, it was observed that 31 miRNAs were upregulated and 16 miRNAs were downregulated in exosomes. Also, 341 overlapped genes from miRNAs targets and mRNA-seq were selected for future analysis. GO analysis indicated that overlapped genes were significantly enriched in the leucine transport, MAP kinase phosphatase activity, and glutamine transport. KEGG analysis suggested that the genes were enriched in cellular senescence, p53 signaling pathway and cell cycle. The top ten genes including CCNB1, BUB1, KIF20A, MKI67, FOXM1, BIRC5, NCAPH, ZWINT, GINS2, and ASF1B were identified from the PPI network.
Conclusion: The results identified key miRNAs with altered expression and characterizing key genes in exosomes derived cervical cancer cells. This study will provide novel insights to determine the potential mechanisms associated exosomes derived from cancer cells in cervical cancer progression. |
| first_indexed | 2024-03-09T02:15:49Z |
| format | Article |
| id | doaj.art-60b6fc47e02d4a0f93a87673e45f2a1c |
| institution | Directory Open Access Journal |
| issn | 1680-2993 2008-2363 |
| language | fas |
| last_indexed | 2024-03-09T02:15:49Z |
| publishDate | 2023-10-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Majallah-i Zanān, Māmā̓ī va Nāzā̓ī-i Īrān |
| spelling | doaj.art-60b6fc47e02d4a0f93a87673e45f2a1c2023-12-07T04:18:46ZfasMashhad University of Medical SciencesMajallah-i Zanān, Māmā̓ī va Nāzā̓ī-i Īrān1680-29932008-23632023-10-01268556810.22038/ijogi.2023.2339923399The expression profile of exosomal miRNAs in cervical cancer cells (HeLa) compared to normal cells and evaluating the key genes related to themMarzieh Naderi Boldaji0Kambiz Ahmadi1Somayeh Reiisi2M.Sc. of Cellular and Molecular Biology, Department of Genetics, School of Basic Sciences, Shahrekord University, Shahrekord, Iran.Assistant Professor, Department of Computer Science, School of Mathematical Sciences, Shahrekord University, Shahrekord, Iran.Associate Professor, Department of Genetics, School of Basic Sciences, Shahrekord University, Shahrekord, Iran.Introduction: Cervical cancer is the fourth most common cancer among women worldwide and is a heterogeneous disease. The exosomal miRNAs could be applied as diagnostic biomarkers and therapeutic targets in cervical cancer. The present study was performed with aim to use a bioinformatics analysis to identify key miRNAs and related genes and pathways in exosomes derived from cervical cancer cells. Methods: In this study, the differential expression of exosomal miRNA and RNAs in healthy and HeLa cells was investigated by analyzing the GEO database for miR-seq and RNA-seq in R software. Subsequently, overlapped miRNA target genes and differentially mRNA from exosome were selected and then, GO and KEGG pathway analysis was performed. The overlapped genes were integrated to construct a PPI network and hub genes selection. Results: In the present study, it was observed that 31 miRNAs were upregulated and 16 miRNAs were downregulated in exosomes. Also, 341 overlapped genes from miRNAs targets and mRNA-seq were selected for future analysis. GO analysis indicated that overlapped genes were significantly enriched in the leucine transport, MAP kinase phosphatase activity, and glutamine transport. KEGG analysis suggested that the genes were enriched in cellular senescence, p53 signaling pathway and cell cycle. The top ten genes including CCNB1, BUB1, KIF20A, MKI67, FOXM1, BIRC5, NCAPH, ZWINT, GINS2, and ASF1B were identified from the PPI network. Conclusion: The results identified key miRNAs with altered expression and characterizing key genes in exosomes derived cervical cancer cells. This study will provide novel insights to determine the potential mechanisms associated exosomes derived from cancer cells in cervical cancer progression.https://ijogi.mums.ac.ir/article_23399_f0510515c385c5ccd742fc8ce1d0829b.pdfbiomarkercervical cancerexosomemirnamrnasignaling pathway |
| spellingShingle | Marzieh Naderi Boldaji Kambiz Ahmadi Somayeh Reiisi The expression profile of exosomal miRNAs in cervical cancer cells (HeLa) compared to normal cells and evaluating the key genes related to them Majallah-i Zanān, Māmā̓ī va Nāzā̓ī-i Īrān biomarker cervical cancer exosome mirna mrna signaling pathway |
| title | The expression profile of exosomal miRNAs in cervical cancer cells (HeLa) compared to normal cells and evaluating the key genes related to them |
| title_full | The expression profile of exosomal miRNAs in cervical cancer cells (HeLa) compared to normal cells and evaluating the key genes related to them |
| title_fullStr | The expression profile of exosomal miRNAs in cervical cancer cells (HeLa) compared to normal cells and evaluating the key genes related to them |
| title_full_unstemmed | The expression profile of exosomal miRNAs in cervical cancer cells (HeLa) compared to normal cells and evaluating the key genes related to them |
| title_short | The expression profile of exosomal miRNAs in cervical cancer cells (HeLa) compared to normal cells and evaluating the key genes related to them |
| title_sort | expression profile of exosomal mirnas in cervical cancer cells hela compared to normal cells and evaluating the key genes related to them |
| topic | biomarker cervical cancer exosome mirna mrna signaling pathway |
| url | https://ijogi.mums.ac.ir/article_23399_f0510515c385c5ccd742fc8ce1d0829b.pdf |
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