Synthesis of Silver Nanoparticles using Methanol Extract of Bunium Persicum and the Evaluation of its Cytotoxic, Antileishmanial, and Antimicrobial Activities
Introduction: The unique properties of silver nanoparticles (Ag-NPs) produced using plant extract make them attractive for use in medical and industrial applications. Bunium persicum from the Apiaceae family is native to Iran, Afghanistan, Pakistan, and some Central Asian countries, which is locally...
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| Format: | Article |
| Language: | fas |
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Ilam University of Medical Sciences
2023-02-01
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| Series: | Majallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Īlām |
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| Online Access: | http://sjimu.medilam.ac.ir/article-1-7447-en.html |
| _version_ | 1811160094460608512 |
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| author | Fatemeh Sharifi Neda MohamadI Sara Soltanian Mohsen Doostmohammadi |
| author_facet | Fatemeh Sharifi Neda MohamadI Sara Soltanian Mohsen Doostmohammadi |
| author_sort | Fatemeh Sharifi |
| collection | DOAJ |
| description | Introduction: The unique properties of silver nanoparticles (Ag-NPs) produced using plant extract make them attractive for use in medical and industrial applications. Bunium persicum from the Apiaceae family is native to Iran, Afghanistan, Pakistan, and some Central Asian countries, which is locally known as "Kermanin Black Cumin" in Iran. In this study, Ag-NPs were synthesized using methanol extract of B. persicum as the regenerating and stabilizing agent for the first time and were followed by the characterization and evaluation of its biological potency.
Material & Methods: Synthesis of Ag-NPs was conducted using the B. persicum extract. Ultraviolet-visible (UV-VIS) spectroscopy was used to detect the presence of nanoparticles. Scanning Electron Microscope (SEM) was also employed to visualize the surface morphology, shape, and size distribution of nanoparticles. Fourier Transform Infrared Spectroscopy (FTIR) is very sensitive to the chemical surface of nanoparticles and was utilized to identify functional groups in the nanoparticles. Cytotoxicity against cancer cell lines and antileishmanial activities were investigated using MTT assay, and the well diffusion method was used to detect the antibacterial property of the synthesized nanoparticles.
Findings: UV-VIS spectrum exhibits an absorption band at around 400-450 nm suggesting the formation of biological Ag-NPs. The size and morphological properties of nanoparticles were assessed by SEM which showed that particles have spherical shapes with a diameter of about 20-70 nm. Ag-NPs showed cytotoxicity against human glioblastoma cancer cell line A-172 (IC50:7.2 µg/ml) and breast cancer cell line MCF-7 (IC50:7.6 µg/ml) after 48 h treatment. Ag-NPs presented antimicrobial activity against Gram-positive and Gram-negative bacteria. The present study confirmed good antileishmanial activity against the promastigote and amastigote stages of Leishmania major. The IC50 values of Ag-NPs and Glucantime® were 73.89 and 16.17μg/mL for promastigote, as well as 171.02 and 398.21 μg/mL for amastigotes assays, respectively.
Discussion & Conclusion: The extract of B. persicum has the ability to reduce Ag+ ions to Ag nanoparticles. Moreover, the fabricated Ag-NPs have good cytotoxicity, antibacterial, and antileishmanial activities. |
| first_indexed | 2024-04-10T05:51:49Z |
| format | Article |
| id | doaj.art-60ba0c14d83f4e5889f9ad1a5bab4873 |
| institution | Directory Open Access Journal |
| issn | 1563-4728 2588-3135 |
| language | fas |
| last_indexed | 2024-04-10T05:51:49Z |
| publishDate | 2023-02-01 |
| publisher | Ilam University of Medical Sciences |
| record_format | Article |
| series | Majallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Īlām |
| spelling | doaj.art-60ba0c14d83f4e5889f9ad1a5bab48732023-03-04T07:26:17ZfasIlam University of Medical SciencesMajallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Īlām1563-47282588-31352023-02-01306920Synthesis of Silver Nanoparticles using Methanol Extract of Bunium Persicum and the Evaluation of its Cytotoxic, Antileishmanial, and Antimicrobial ActivitiesFatemeh Sharifi0Neda MohamadI1Sara Soltanian2Mohsen Doostmohammadi3 Research Center of Tropical and Infectious Diseases, Kerman University of Medical Sciences, Kerman, Iran Herbal and Traditional Medicines Research Center, Kerman University of Medical Sciences, Kerman, Iran Dept of Biology, Faculty of Sciences, Shahid Bahonar University of Kerman, Kerman, Iran Pharmaceutic Research Center, Institute of Neuropharmacology, Kerman University of Medical Science, Kerman, Iran Introduction: The unique properties of silver nanoparticles (Ag-NPs) produced using plant extract make them attractive for use in medical and industrial applications. Bunium persicum from the Apiaceae family is native to Iran, Afghanistan, Pakistan, and some Central Asian countries, which is locally known as "Kermanin Black Cumin" in Iran. In this study, Ag-NPs were synthesized using methanol extract of B. persicum as the regenerating and stabilizing agent for the first time and were followed by the characterization and evaluation of its biological potency. Material & Methods: Synthesis of Ag-NPs was conducted using the B. persicum extract. Ultraviolet-visible (UV-VIS) spectroscopy was used to detect the presence of nanoparticles. Scanning Electron Microscope (SEM) was also employed to visualize the surface morphology, shape, and size distribution of nanoparticles. Fourier Transform Infrared Spectroscopy (FTIR) is very sensitive to the chemical surface of nanoparticles and was utilized to identify functional groups in the nanoparticles. Cytotoxicity against cancer cell lines and antileishmanial activities were investigated using MTT assay, and the well diffusion method was used to detect the antibacterial property of the synthesized nanoparticles. Findings: UV-VIS spectrum exhibits an absorption band at around 400-450 nm suggesting the formation of biological Ag-NPs. The size and morphological properties of nanoparticles were assessed by SEM which showed that particles have spherical shapes with a diameter of about 20-70 nm. Ag-NPs showed cytotoxicity against human glioblastoma cancer cell line A-172 (IC50:7.2 µg/ml) and breast cancer cell line MCF-7 (IC50:7.6 µg/ml) after 48 h treatment. Ag-NPs presented antimicrobial activity against Gram-positive and Gram-negative bacteria. The present study confirmed good antileishmanial activity against the promastigote and amastigote stages of Leishmania major. The IC50 values of Ag-NPs and Glucantime® were 73.89 and 16.17μg/mL for promastigote, as well as 171.02 and 398.21 μg/mL for amastigotes assays, respectively. Discussion & Conclusion: The extract of B. persicum has the ability to reduce Ag+ ions to Ag nanoparticles. Moreover, the fabricated Ag-NPs have good cytotoxicity, antibacterial, and antileishmanial activities.http://sjimu.medilam.ac.ir/article-1-7447-en.htmlantibacterial activityantileishmanial effectsbunium persicumcytotoxicitygreen synthesissilver nanoparticles |
| spellingShingle | Fatemeh Sharifi Neda MohamadI Sara Soltanian Mohsen Doostmohammadi Synthesis of Silver Nanoparticles using Methanol Extract of Bunium Persicum and the Evaluation of its Cytotoxic, Antileishmanial, and Antimicrobial Activities Majallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Īlām antibacterial activity antileishmanial effects bunium persicum cytotoxicity green synthesis silver nanoparticles |
| title | Synthesis of Silver Nanoparticles using Methanol Extract of Bunium Persicum and the Evaluation of its Cytotoxic, Antileishmanial, and Antimicrobial Activities |
| title_full | Synthesis of Silver Nanoparticles using Methanol Extract of Bunium Persicum and the Evaluation of its Cytotoxic, Antileishmanial, and Antimicrobial Activities |
| title_fullStr | Synthesis of Silver Nanoparticles using Methanol Extract of Bunium Persicum and the Evaluation of its Cytotoxic, Antileishmanial, and Antimicrobial Activities |
| title_full_unstemmed | Synthesis of Silver Nanoparticles using Methanol Extract of Bunium Persicum and the Evaluation of its Cytotoxic, Antileishmanial, and Antimicrobial Activities |
| title_short | Synthesis of Silver Nanoparticles using Methanol Extract of Bunium Persicum and the Evaluation of its Cytotoxic, Antileishmanial, and Antimicrobial Activities |
| title_sort | synthesis of silver nanoparticles using methanol extract of bunium persicum and the evaluation of its cytotoxic antileishmanial and antimicrobial activities |
| topic | antibacterial activity antileishmanial effects bunium persicum cytotoxicity green synthesis silver nanoparticles |
| url | http://sjimu.medilam.ac.ir/article-1-7447-en.html |
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