Reduced inflammatory phenotype in microglia derived from neonatal rat spinal cord versus brain.
Microglia are the primary immune cells of the central nervous system (CNS). Membrane bound sensors on their processes monitor the extracellular environment and respond to perturbations of the CNS such as injury or infection. Once activated, microglia play a crucial role in determining neuronal survi...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4051776?pdf=render |
| _version_ | 1818907780190306304 |
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| author | Sam Joshva Baskar Jesudasan Kathryn G Todd Ian R Winship |
| author_facet | Sam Joshva Baskar Jesudasan Kathryn G Todd Ian R Winship |
| author_sort | Sam Joshva Baskar Jesudasan |
| collection | DOAJ |
| description | Microglia are the primary immune cells of the central nervous system (CNS). Membrane bound sensors on their processes monitor the extracellular environment and respond to perturbations of the CNS such as injury or infection. Once activated, microglia play a crucial role in determining neuronal survival. Recent studies suggest that microglial functional response properties vary across different regions of the CNS. However, the activation profiles of microglia derived from the spinal cord have not been evaluated against brain microglia in vitro. Here, we studied the morphological properties and secretion of inflammatory and trophic effectors by microglia derived from the brain or spinal cord of neonatal rats under basal culture conditions and after activation with lipopolysaccharide (LPS). Our results demonstrate that spinal microglia assume a less inflammatory phenotype after LPS activation, with reduced release of the inflammatory effectors tumor necrosis factor alpha, interleukin-1 beta, and nitric oxide, a less amoeboid morphology, and reduced phagocytosis relative to brain-derived microglia. Phenotypic differences between brain and spinal microglia are an important consideration when evaluating anti-inflammatory or immunomodulatory therapies for brain versus spinal injury. |
| first_indexed | 2024-12-19T22:00:33Z |
| format | Article |
| id | doaj.art-60bbcba6e3c045948a9bceef2b91a65a |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-19T22:00:33Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-60bbcba6e3c045948a9bceef2b91a65a2022-12-21T20:04:09ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0196e9944310.1371/journal.pone.0099443Reduced inflammatory phenotype in microglia derived from neonatal rat spinal cord versus brain.Sam Joshva Baskar JesudasanKathryn G ToddIan R WinshipMicroglia are the primary immune cells of the central nervous system (CNS). Membrane bound sensors on their processes monitor the extracellular environment and respond to perturbations of the CNS such as injury or infection. Once activated, microglia play a crucial role in determining neuronal survival. Recent studies suggest that microglial functional response properties vary across different regions of the CNS. However, the activation profiles of microglia derived from the spinal cord have not been evaluated against brain microglia in vitro. Here, we studied the morphological properties and secretion of inflammatory and trophic effectors by microglia derived from the brain or spinal cord of neonatal rats under basal culture conditions and after activation with lipopolysaccharide (LPS). Our results demonstrate that spinal microglia assume a less inflammatory phenotype after LPS activation, with reduced release of the inflammatory effectors tumor necrosis factor alpha, interleukin-1 beta, and nitric oxide, a less amoeboid morphology, and reduced phagocytosis relative to brain-derived microglia. Phenotypic differences between brain and spinal microglia are an important consideration when evaluating anti-inflammatory or immunomodulatory therapies for brain versus spinal injury.http://europepmc.org/articles/PMC4051776?pdf=render |
| spellingShingle | Sam Joshva Baskar Jesudasan Kathryn G Todd Ian R Winship Reduced inflammatory phenotype in microglia derived from neonatal rat spinal cord versus brain. PLoS ONE |
| title | Reduced inflammatory phenotype in microglia derived from neonatal rat spinal cord versus brain. |
| title_full | Reduced inflammatory phenotype in microglia derived from neonatal rat spinal cord versus brain. |
| title_fullStr | Reduced inflammatory phenotype in microglia derived from neonatal rat spinal cord versus brain. |
| title_full_unstemmed | Reduced inflammatory phenotype in microglia derived from neonatal rat spinal cord versus brain. |
| title_short | Reduced inflammatory phenotype in microglia derived from neonatal rat spinal cord versus brain. |
| title_sort | reduced inflammatory phenotype in microglia derived from neonatal rat spinal cord versus brain |
| url | http://europepmc.org/articles/PMC4051776?pdf=render |
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