Implication of the Whitefly Protein Vps Twenty Associated 1 (Vta1) in the Transmission of Cotton Leaf Curl Multan Virus
Cotton leaf curl Multan virus (CLCuMuV) is one of the major casual agents of cotton leaf curl disease. Previous studies show that two indigenous whitefly species of the <i>Bemisia tabaci</i> complex, Asia II 1 and Asia II 7, are able to transmit CLCuMuV, but the molecular mechanisms unde...
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MDPI AG
2021-02-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/9/2/304 |
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| author | Yao Chi Li-Long Pan Shu-Sheng Liu Shahid Mansoor Xiao-Wei Wang |
| author_facet | Yao Chi Li-Long Pan Shu-Sheng Liu Shahid Mansoor Xiao-Wei Wang |
| author_sort | Yao Chi |
| collection | DOAJ |
| description | Cotton leaf curl Multan virus (CLCuMuV) is one of the major casual agents of cotton leaf curl disease. Previous studies show that two indigenous whitefly species of the <i>Bemisia tabaci</i> complex, Asia II 1 and Asia II 7, are able to transmit CLCuMuV, but the molecular mechanisms underlying the transmission are poorly known. In this study, we attempted to identify the whitefly proteins involved in CLCuMuV transmission. First, using a yeast two-hybrid system, we identified 54 candidate proteins of Asia II 1 that putatively can interact with the coat protein of CLCuMuV. Second, we examined interactions between the CLCuMuV coat protein and several whitefly proteins, including vacuolar protein sorting-associated protein (Vps) twenty associated 1 (Vta1). Third, using RNA interference, we found that Vta1 positively regulated CLCuMuV acquisition and transmission by the Asia II 1 whitefly. In addition, we showed that the interaction between the CLCuMuV coat protein and Vta1 from the whitefly Middle East-Asia Minor (MEAM1), a poor vector of CLCuMuV, was much weaker than that between Asia II 1 Vta1 and the CLCuMuV coat protein. Silencing of <i>Vta1</i> in MEAM1 did not affect the quantity of CLCuMuV acquired by the whitefly. Taken together, our results suggest that Vta1 may play an important role in the transmission of CLCuMuV by the whitefly. |
| first_indexed | 2024-03-09T06:05:21Z |
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| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T06:05:21Z |
| publishDate | 2021-02-01 |
| publisher | MDPI AG |
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| series | Microorganisms |
| spelling | doaj.art-60beb1d66b5c4b4696e15fb7fc209c6b2023-12-03T12:03:53ZengMDPI AGMicroorganisms2076-26072021-02-019230410.3390/microorganisms9020304Implication of the Whitefly Protein Vps Twenty Associated 1 (Vta1) in the Transmission of Cotton Leaf Curl Multan VirusYao Chi0Li-Long Pan1Shu-Sheng Liu2Shahid Mansoor3Xiao-Wei Wang4Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, ChinaMinistry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, ChinaMinistry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, ChinaNational Institute for Biotechnology and Genetic Engineering, Faisalabad 38000, PakistanMinistry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, ChinaCotton leaf curl Multan virus (CLCuMuV) is one of the major casual agents of cotton leaf curl disease. Previous studies show that two indigenous whitefly species of the <i>Bemisia tabaci</i> complex, Asia II 1 and Asia II 7, are able to transmit CLCuMuV, but the molecular mechanisms underlying the transmission are poorly known. In this study, we attempted to identify the whitefly proteins involved in CLCuMuV transmission. First, using a yeast two-hybrid system, we identified 54 candidate proteins of Asia II 1 that putatively can interact with the coat protein of CLCuMuV. Second, we examined interactions between the CLCuMuV coat protein and several whitefly proteins, including vacuolar protein sorting-associated protein (Vps) twenty associated 1 (Vta1). Third, using RNA interference, we found that Vta1 positively regulated CLCuMuV acquisition and transmission by the Asia II 1 whitefly. In addition, we showed that the interaction between the CLCuMuV coat protein and Vta1 from the whitefly Middle East-Asia Minor (MEAM1), a poor vector of CLCuMuV, was much weaker than that between Asia II 1 Vta1 and the CLCuMuV coat protein. Silencing of <i>Vta1</i> in MEAM1 did not affect the quantity of CLCuMuV acquired by the whitefly. Taken together, our results suggest that Vta1 may play an important role in the transmission of CLCuMuV by the whitefly.https://www.mdpi.com/2076-2607/9/2/304whiteflybegomovirusVta1virus transmissioncoat proteins |
| spellingShingle | Yao Chi Li-Long Pan Shu-Sheng Liu Shahid Mansoor Xiao-Wei Wang Implication of the Whitefly Protein Vps Twenty Associated 1 (Vta1) in the Transmission of Cotton Leaf Curl Multan Virus Microorganisms whitefly begomovirus Vta1 virus transmission coat proteins |
| title | Implication of the Whitefly Protein Vps Twenty Associated 1 (Vta1) in the Transmission of Cotton Leaf Curl Multan Virus |
| title_full | Implication of the Whitefly Protein Vps Twenty Associated 1 (Vta1) in the Transmission of Cotton Leaf Curl Multan Virus |
| title_fullStr | Implication of the Whitefly Protein Vps Twenty Associated 1 (Vta1) in the Transmission of Cotton Leaf Curl Multan Virus |
| title_full_unstemmed | Implication of the Whitefly Protein Vps Twenty Associated 1 (Vta1) in the Transmission of Cotton Leaf Curl Multan Virus |
| title_short | Implication of the Whitefly Protein Vps Twenty Associated 1 (Vta1) in the Transmission of Cotton Leaf Curl Multan Virus |
| title_sort | implication of the whitefly protein vps twenty associated 1 vta1 in the transmission of cotton leaf curl multan virus |
| topic | whitefly begomovirus Vta1 virus transmission coat proteins |
| url | https://www.mdpi.com/2076-2607/9/2/304 |
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