Polyphosphate Kinase Mediates Antibiotic Tolerance in Extraintestinal Pathogenic Escherichia coli PCN033
Extraintestinal pathogenic Escherichia coli (ExPEC) causes a variety of acute infections in its hosts, and multidrug-resistant strains present significant challenges to public health and animal husbandry. Therefore, it is necessary to explore new drug targets to control E. coli epidemics. Previous s...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2016-05-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.00724/full |
| _version_ | 1818148521153396736 |
|---|---|
| author | Jing eChen Lijie eSu Xiangru eWang Tao eZhang Feng eLiu Huanchun eChen Huanchun eChen Chen eTan Chen eTan |
| author_facet | Jing eChen Lijie eSu Xiangru eWang Tao eZhang Feng eLiu Huanchun eChen Huanchun eChen Chen eTan Chen eTan |
| author_sort | Jing eChen |
| collection | DOAJ |
| description | Extraintestinal pathogenic Escherichia coli (ExPEC) causes a variety of acute infections in its hosts, and multidrug-resistant strains present significant challenges to public health and animal husbandry. Therefore, it is necessary to explore new drug targets to control E. coli epidemics. Previous studies have reported that ppk mutants of Burkholderia pseudomallei and Mycobacterium tuberculosis are more susceptible than the wild types (WTs) to stress. Therefore, we investigated the stress response to antibiotics mediated by polyphosphate kinase (PPK) in ExPEC strain PCN033. We observed that planktonic cells of a ppk knockout strain (Δppk) were more susceptible to antibiotics than was WT. However, biofilm-grown Δppk cells showed similar susceptibility to that of the WT and were more tolerant than the planktonic cells. During the planktonic lifestyle, the expression of genes involved in antibiotic tolerance (including resistance-conferring genes,and antibiotic influx and efflux genes) did not change in the Δppk mutant without antibiotic treatment. However, the resistance-conferring gene bla and efflux genes were upregulated more in the WT than in the Δppk mutant by treatment with tazobactam. After treatment with gentamycin, the efflux genes and influx genes were upregulated and downregulated, respectively, more in the WT than in the Δppk mutant. The expression of genes involved in biofilm regulation also changed after treatment with tazobactam or gentamycin, and which is consistent with the results of the biofilm formation. Together, these observations indicate that PPK is important for the antibiotic stress response during the planktonic growth of ExPEC and might be a potential drug target in bacteria. |
| first_indexed | 2024-12-11T12:52:28Z |
| format | Article |
| id | doaj.art-60bfaa490dd7460fb5348870e85d156f |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-11T12:52:28Z |
| publishDate | 2016-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-60bfaa490dd7460fb5348870e85d156f2022-12-22T01:06:39ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2016-05-01710.3389/fmicb.2016.00724179940Polyphosphate Kinase Mediates Antibiotic Tolerance in Extraintestinal Pathogenic Escherichia coli PCN033Jing eChen0Lijie eSu1Xiangru eWang2Tao eZhang3Feng eLiu4Huanchun eChen5Huanchun eChen6Chen eTan7Chen eTan8Huazhong Agricultural UniversityGuangzhou Medical UniversityHuazhong Agricultural UniversityHuazhong Agricultural UniversityHuazhong Agricultural UniversityHuazhong Agricultural UniversityThe Cooperative Innovation Center for Sustainable Pig ProductionHuazhong Agricultural UniversityThe Cooperative Innovation Center for Sustainable Pig ProductionExtraintestinal pathogenic Escherichia coli (ExPEC) causes a variety of acute infections in its hosts, and multidrug-resistant strains present significant challenges to public health and animal husbandry. Therefore, it is necessary to explore new drug targets to control E. coli epidemics. Previous studies have reported that ppk mutants of Burkholderia pseudomallei and Mycobacterium tuberculosis are more susceptible than the wild types (WTs) to stress. Therefore, we investigated the stress response to antibiotics mediated by polyphosphate kinase (PPK) in ExPEC strain PCN033. We observed that planktonic cells of a ppk knockout strain (Δppk) were more susceptible to antibiotics than was WT. However, biofilm-grown Δppk cells showed similar susceptibility to that of the WT and were more tolerant than the planktonic cells. During the planktonic lifestyle, the expression of genes involved in antibiotic tolerance (including resistance-conferring genes,and antibiotic influx and efflux genes) did not change in the Δppk mutant without antibiotic treatment. However, the resistance-conferring gene bla and efflux genes were upregulated more in the WT than in the Δppk mutant by treatment with tazobactam. After treatment with gentamycin, the efflux genes and influx genes were upregulated and downregulated, respectively, more in the WT than in the Δppk mutant. The expression of genes involved in biofilm regulation also changed after treatment with tazobactam or gentamycin, and which is consistent with the results of the biofilm formation. Together, these observations indicate that PPK is important for the antibiotic stress response during the planktonic growth of ExPEC and might be a potential drug target in bacteria.http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.00724/fullGene ExpressionqPCRRNA-Seqantibiotic resistanceExPECPpk |
| spellingShingle | Jing eChen Lijie eSu Xiangru eWang Tao eZhang Feng eLiu Huanchun eChen Huanchun eChen Chen eTan Chen eTan Polyphosphate Kinase Mediates Antibiotic Tolerance in Extraintestinal Pathogenic Escherichia coli PCN033 Frontiers in Microbiology Gene Expression qPCR RNA-Seq antibiotic resistance ExPEC Ppk |
| title | Polyphosphate Kinase Mediates Antibiotic Tolerance in Extraintestinal Pathogenic Escherichia coli PCN033 |
| title_full | Polyphosphate Kinase Mediates Antibiotic Tolerance in Extraintestinal Pathogenic Escherichia coli PCN033 |
| title_fullStr | Polyphosphate Kinase Mediates Antibiotic Tolerance in Extraintestinal Pathogenic Escherichia coli PCN033 |
| title_full_unstemmed | Polyphosphate Kinase Mediates Antibiotic Tolerance in Extraintestinal Pathogenic Escherichia coli PCN033 |
| title_short | Polyphosphate Kinase Mediates Antibiotic Tolerance in Extraintestinal Pathogenic Escherichia coli PCN033 |
| title_sort | polyphosphate kinase mediates antibiotic tolerance in extraintestinal pathogenic escherichia coli pcn033 |
| topic | Gene Expression qPCR RNA-Seq antibiotic resistance ExPEC Ppk |
| url | http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.00724/full |
| work_keys_str_mv | AT jingechen polyphosphatekinasemediatesantibiotictoleranceinextraintestinalpathogenicescherichiacolipcn033 AT lijieesu polyphosphatekinasemediatesantibiotictoleranceinextraintestinalpathogenicescherichiacolipcn033 AT xiangruewang polyphosphatekinasemediatesantibiotictoleranceinextraintestinalpathogenicescherichiacolipcn033 AT taoezhang polyphosphatekinasemediatesantibiotictoleranceinextraintestinalpathogenicescherichiacolipcn033 AT fengeliu polyphosphatekinasemediatesantibiotictoleranceinextraintestinalpathogenicescherichiacolipcn033 AT huanchunechen polyphosphatekinasemediatesantibiotictoleranceinextraintestinalpathogenicescherichiacolipcn033 AT huanchunechen polyphosphatekinasemediatesantibiotictoleranceinextraintestinalpathogenicescherichiacolipcn033 AT chenetan polyphosphatekinasemediatesantibiotictoleranceinextraintestinalpathogenicescherichiacolipcn033 AT chenetan polyphosphatekinasemediatesantibiotictoleranceinextraintestinalpathogenicescherichiacolipcn033 |