Development and characterization of monoclonal antibodies against the extracellular domain of African swine fever virus structural protein, CD2v
African swine fever virus (ASFV), a DNA double-stranded virus with high infectivity and mortality, causing a devastating blow to the pig industry and the world economy. The CD2v protein is an essential immunoprotective protein of ASFV. In this study, we expressed the extracellular region of the CD2v...
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Frontiers Media S.A.
2022-11-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.1056117/full |
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| author | Siyuan Liu Siyuan Liu Peiyang Ding Yongkun Du Yongkun Du Dongna Ren Yilan Chen Minghui Li Minghui Li Xueke Sun Siqiao Wang Zejie Chang Ruiqi Li Gaiping Zhang Gaiping Zhang Gaiping Zhang Gaiping Zhang |
| author_facet | Siyuan Liu Siyuan Liu Peiyang Ding Yongkun Du Yongkun Du Dongna Ren Yilan Chen Minghui Li Minghui Li Xueke Sun Siqiao Wang Zejie Chang Ruiqi Li Gaiping Zhang Gaiping Zhang Gaiping Zhang Gaiping Zhang |
| author_sort | Siyuan Liu |
| collection | DOAJ |
| description | African swine fever virus (ASFV), a DNA double-stranded virus with high infectivity and mortality, causing a devastating blow to the pig industry and the world economy. The CD2v protein is an essential immunoprotective protein of ASFV. In this study, we expressed the extracellular region of the CD2v protein in the 293F expression system to achieve proper glycosylation. Monoclonal antibodies (mAbs) were prepared by immunizing mice with the recombinant CD2v protein. Eventually, four mAbs that target the extracellular region of the ASFV CD2v protein were obtained. All four mAbs responded well to the ASFV HLJ/18 strain and recognized the same linear epitope, 154SILE157. The specific shortest amino acid sequence of this epitope has been accurately identified for the first time. Meaningfully, the 154SILE157 epitope was highly conformed in the ASFV Chinese epidemic strain and Georgia2008/1 strains according to the analysis of the conservation and have a fair protective effect. These findings contribute to further understanding of the protein function of CD2v and provide potential support for the development of diagnostic tools and vaccines for ASFV. |
| first_indexed | 2024-04-11T06:51:05Z |
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| id | doaj.art-60c02e0e97294f84be1f90b8b2ed0c62 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-11T06:51:05Z |
| publishDate | 2022-11-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Microbiology |
| spelling | doaj.art-60c02e0e97294f84be1f90b8b2ed0c622022-12-22T04:39:12ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-11-011310.3389/fmicb.2022.10561171056117Development and characterization of monoclonal antibodies against the extracellular domain of African swine fever virus structural protein, CD2vSiyuan Liu0Siyuan Liu1Peiyang Ding2Yongkun Du3Yongkun Du4Dongna Ren5Yilan Chen6Minghui Li7Minghui Li8Xueke Sun9Siqiao Wang10Zejie Chang11Ruiqi Li12Gaiping Zhang13Gaiping Zhang14Gaiping Zhang15Gaiping Zhang16College of Animal Medicine, Henan Agricultural University, Zhengzhou, ChinaHenan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou, ChinaCollege of Life Sciences, Zhengzhou University, Zhengzhou, ChinaCollege of Animal Medicine, Henan Agricultural University, Zhengzhou, ChinaHenan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou, ChinaHenan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou, ChinaHenan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou, ChinaCollege of Animal Medicine, Henan Agricultural University, Zhengzhou, ChinaHenan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou, ChinaHenan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou, ChinaHenan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou, ChinaHenan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou, ChinaHenan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou, ChinaCollege of Animal Medicine, Henan Agricultural University, Zhengzhou, ChinaHenan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou, ChinaCollege of Life Sciences, Zhengzhou University, Zhengzhou, ChinaJiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Zhengzhou, ChinaAfrican swine fever virus (ASFV), a DNA double-stranded virus with high infectivity and mortality, causing a devastating blow to the pig industry and the world economy. The CD2v protein is an essential immunoprotective protein of ASFV. In this study, we expressed the extracellular region of the CD2v protein in the 293F expression system to achieve proper glycosylation. Monoclonal antibodies (mAbs) were prepared by immunizing mice with the recombinant CD2v protein. Eventually, four mAbs that target the extracellular region of the ASFV CD2v protein were obtained. All four mAbs responded well to the ASFV HLJ/18 strain and recognized the same linear epitope, 154SILE157. The specific shortest amino acid sequence of this epitope has been accurately identified for the first time. Meaningfully, the 154SILE157 epitope was highly conformed in the ASFV Chinese epidemic strain and Georgia2008/1 strains according to the analysis of the conservation and have a fair protective effect. These findings contribute to further understanding of the protein function of CD2v and provide potential support for the development of diagnostic tools and vaccines for ASFV.https://www.frontiersin.org/articles/10.3389/fmicb.2022.1056117/fullAfrican swine fever virusCD2v proteinmonoclonal antibodiesB-cell epitopeextracellular |
| spellingShingle | Siyuan Liu Siyuan Liu Peiyang Ding Yongkun Du Yongkun Du Dongna Ren Yilan Chen Minghui Li Minghui Li Xueke Sun Siqiao Wang Zejie Chang Ruiqi Li Gaiping Zhang Gaiping Zhang Gaiping Zhang Gaiping Zhang Development and characterization of monoclonal antibodies against the extracellular domain of African swine fever virus structural protein, CD2v Frontiers in Microbiology African swine fever virus CD2v protein monoclonal antibodies B-cell epitope extracellular |
| title | Development and characterization of monoclonal antibodies against the extracellular domain of African swine fever virus structural protein, CD2v |
| title_full | Development and characterization of monoclonal antibodies against the extracellular domain of African swine fever virus structural protein, CD2v |
| title_fullStr | Development and characterization of monoclonal antibodies against the extracellular domain of African swine fever virus structural protein, CD2v |
| title_full_unstemmed | Development and characterization of monoclonal antibodies against the extracellular domain of African swine fever virus structural protein, CD2v |
| title_short | Development and characterization of monoclonal antibodies against the extracellular domain of African swine fever virus structural protein, CD2v |
| title_sort | development and characterization of monoclonal antibodies against the extracellular domain of african swine fever virus structural protein cd2v |
| topic | African swine fever virus CD2v protein monoclonal antibodies B-cell epitope extracellular |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.1056117/full |
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