Association of interleukin 2, interleukin 12, and interferon-γ with intervertebral disc degeneration in Iranian population
Abstract Background Intervertebral disc degeneration (IVDD) is an age-related degenerative disease, presenting with low back pain or radicular pain. The inflammatory changes would occur in discs in the process of IVDD. Therefore, the inflammatory and anti-inflammatory cytokines, as well as their res...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2020-07-01
|
| Series: | BMC Medical Genetics |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12881-020-01081-3 |
| _version_ | 1818741511842430976 |
|---|---|
| author | Sara Hanaei Sina Abdollahzade Maryam Sadr Mohammad Hossein Mirbolouk Ehsan Fattahi Alireza Khoshnevisan Nima Rezaei |
| author_facet | Sara Hanaei Sina Abdollahzade Maryam Sadr Mohammad Hossein Mirbolouk Ehsan Fattahi Alireza Khoshnevisan Nima Rezaei |
| author_sort | Sara Hanaei |
| collection | DOAJ |
| description | Abstract Background Intervertebral disc degeneration (IVDD) is an age-related degenerative disease, presenting with low back pain or radicular pain. The inflammatory changes would occur in discs in the process of IVDD. Therefore, the inflammatory and anti-inflammatory cytokines, as well as their respective genes, have been proposed to play roles in pathophysiology of disease. This study has been conducted to elucidate the role of IL-2, IL-12, and IFN-γ single nucleotide polymorphisms (SNP) in this disease. Method Seventy-six patients who were diagnosed with IVDD and 140 healthy controls who complied with eligibility criteria were included. A total volume of 5 cc peripheral blood was obtained from each participant to investigate the IL-2 + 166G/T, IL-2 -330G/T, IL-12 − 1188A/C, and IFN-γ +847A/T SNPs through PCR-SSP method. Results The ‘TG’ and ‘TT’ genotypes of IL-2 − 330G/T polymorphism were significantly more common among patients and healthy controls respectively. The ‘GT’ and ‘TT’ haplotypes of IL-2 (comprised of -330G/T, and + 166G/T SNPs) were also more common among patients and controls respectively. Conclusion This study indicated the significant role of IL-2 genotypes and haplotypes in IVDD. These SNPs were differently distributed in patients and controls. Therefore, alteration in the structure of IL-2 gene could play an important role in pathophysiology of IVDD. |
| first_indexed | 2024-12-18T01:57:48Z |
| format | Article |
| id | doaj.art-60c3bf102c3747c09f0b072ce68bcb38 |
| institution | Directory Open Access Journal |
| issn | 1471-2350 |
| language | English |
| last_indexed | 2024-12-18T01:57:48Z |
| publishDate | 2020-07-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Medical Genetics |
| spelling | doaj.art-60c3bf102c3747c09f0b072ce68bcb382022-12-21T21:24:52ZengBMCBMC Medical Genetics1471-23502020-07-012111710.1186/s12881-020-01081-3Association of interleukin 2, interleukin 12, and interferon-γ with intervertebral disc degeneration in Iranian populationSara Hanaei0Sina Abdollahzade1Maryam Sadr2Mohammad Hossein Mirbolouk3Ehsan Fattahi4Alireza Khoshnevisan5Nima Rezaei6Molecular Immunology Research Center, Tehran University of Medical SciencesDivision of neurosurgery, Department of surgery, Rajayi Hospital, Qazvin University of Medical SciencesMolecular Immunology Research Center, Tehran University of Medical SciencesDepartment of Neurosurgery, Shariati Hospital, Tehran University of Medical SciencesDepartment of Neurosurgery, Shariati Hospital, Tehran University of Medical SciencesDepartment of Neurosurgery, Shariati Hospital, Tehran University of Medical SciencesResearch Center for Immunodeficiencies, Children’s Medical Center, Tehran University of Medical SciencesAbstract Background Intervertebral disc degeneration (IVDD) is an age-related degenerative disease, presenting with low back pain or radicular pain. The inflammatory changes would occur in discs in the process of IVDD. Therefore, the inflammatory and anti-inflammatory cytokines, as well as their respective genes, have been proposed to play roles in pathophysiology of disease. This study has been conducted to elucidate the role of IL-2, IL-12, and IFN-γ single nucleotide polymorphisms (SNP) in this disease. Method Seventy-six patients who were diagnosed with IVDD and 140 healthy controls who complied with eligibility criteria were included. A total volume of 5 cc peripheral blood was obtained from each participant to investigate the IL-2 + 166G/T, IL-2 -330G/T, IL-12 − 1188A/C, and IFN-γ +847A/T SNPs through PCR-SSP method. Results The ‘TG’ and ‘TT’ genotypes of IL-2 − 330G/T polymorphism were significantly more common among patients and healthy controls respectively. The ‘GT’ and ‘TT’ haplotypes of IL-2 (comprised of -330G/T, and + 166G/T SNPs) were also more common among patients and controls respectively. Conclusion This study indicated the significant role of IL-2 genotypes and haplotypes in IVDD. These SNPs were differently distributed in patients and controls. Therefore, alteration in the structure of IL-2 gene could play an important role in pathophysiology of IVDD.http://link.springer.com/article/10.1186/s12881-020-01081-3Intervertebral disc degenerationSingle nucleotide polymorphismInterleukin 2Interleukin 12Interferon γImmunogenetics |
| spellingShingle | Sara Hanaei Sina Abdollahzade Maryam Sadr Mohammad Hossein Mirbolouk Ehsan Fattahi Alireza Khoshnevisan Nima Rezaei Association of interleukin 2, interleukin 12, and interferon-γ with intervertebral disc degeneration in Iranian population BMC Medical Genetics Intervertebral disc degeneration Single nucleotide polymorphism Interleukin 2 Interleukin 12 Interferon γ Immunogenetics |
| title | Association of interleukin 2, interleukin 12, and interferon-γ with intervertebral disc degeneration in Iranian population |
| title_full | Association of interleukin 2, interleukin 12, and interferon-γ with intervertebral disc degeneration in Iranian population |
| title_fullStr | Association of interleukin 2, interleukin 12, and interferon-γ with intervertebral disc degeneration in Iranian population |
| title_full_unstemmed | Association of interleukin 2, interleukin 12, and interferon-γ with intervertebral disc degeneration in Iranian population |
| title_short | Association of interleukin 2, interleukin 12, and interferon-γ with intervertebral disc degeneration in Iranian population |
| title_sort | association of interleukin 2 interleukin 12 and interferon γ with intervertebral disc degeneration in iranian population |
| topic | Intervertebral disc degeneration Single nucleotide polymorphism Interleukin 2 Interleukin 12 Interferon γ Immunogenetics |
| url | http://link.springer.com/article/10.1186/s12881-020-01081-3 |
| work_keys_str_mv | AT sarahanaei associationofinterleukin2interleukin12andinterferongwithintervertebraldiscdegenerationiniranianpopulation AT sinaabdollahzade associationofinterleukin2interleukin12andinterferongwithintervertebraldiscdegenerationiniranianpopulation AT maryamsadr associationofinterleukin2interleukin12andinterferongwithintervertebraldiscdegenerationiniranianpopulation AT mohammadhosseinmirbolouk associationofinterleukin2interleukin12andinterferongwithintervertebraldiscdegenerationiniranianpopulation AT ehsanfattahi associationofinterleukin2interleukin12andinterferongwithintervertebraldiscdegenerationiniranianpopulation AT alirezakhoshnevisan associationofinterleukin2interleukin12andinterferongwithintervertebraldiscdegenerationiniranianpopulation AT nimarezaei associationofinterleukin2interleukin12andinterferongwithintervertebraldiscdegenerationiniranianpopulation |