Antimicrobial Activity and Resistance of Microorganisms Isolated from Honey Bees
Knowledge of composition of bee gut microbiota is important to ensure balanced gut microbiota and improve the health status of bees. The aim of the present study was to detect the microorganisms in gut of honey bee and to study their antimicrobial resistance and antimicrobial activity against three...
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| Format: | Article |
| Language: | English |
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Agroprint Timisoara
2023-09-01
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| Series: | Scientific Papers Animal Science and Biotechnologies |
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| Online Access: | https://spasb.ro/index.php/public_html/article/view/1030 |
| _version_ | 1827288317473849344 |
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| author | Miroslava Kačániová Jaroslav Gasper Margarita Terentjeva Simona Kunová Maciej Kluz Pawel Hanus Czeslaw Puchalski |
| author_facet | Miroslava Kačániová Jaroslav Gasper Margarita Terentjeva Simona Kunová Maciej Kluz Pawel Hanus Czeslaw Puchalski |
| author_sort | Miroslava Kačániová |
| collection | DOAJ |
| description | Knowledge of composition of bee gut microbiota is important to ensure balanced gut microbiota and improve the health status of bees. The aim of the present study was to detect the microorganisms in gut of honey bee and to study their antimicrobial resistance and antimicrobial activity against three essential oils. Content of bees gut was studied with microbiological methods, and isolates were confirmed with MALDI TOF mass spectrometry. Isolated species were tested for antibiotic resistance to ciprofloxacin, levofloxacin, amikacin and gentamicin. For antimicrobial activity, essential oils of Abies alba, Pinus mugo and Pinus sylvestris were used. Gram-positive bacteria Bacillus oleronius, Micrococcus luteus, Staphylococcus epidermidis and three Gram-negative bacteria Pseudomonas oryzihabitans, Serratia fonticola, Serratia marcescens were isolated from bees gut. Antibiotic resistance was found in Gram-negative bacteria, while the Gram-positive bacteria were sensitive to all antibiotic tested. The best antibacterial activity of essential oils found against Gram-positive bacterial strains. Essential oils can be a potential sources of compounds with antimicrobial activity on Gram-positive isolates of bees gut. |
| first_indexed | 2024-04-24T11:29:49Z |
| format | Article |
| id | doaj.art-60c5ce334f174a33b9dec7b3aec6f7f6 |
| institution | Directory Open Access Journal |
| issn | 1841-9364 2344-4576 |
| language | English |
| last_indexed | 2024-04-24T11:29:49Z |
| publishDate | 2023-09-01 |
| publisher | Agroprint Timisoara |
| record_format | Article |
| series | Scientific Papers Animal Science and Biotechnologies |
| spelling | doaj.art-60c5ce334f174a33b9dec7b3aec6f7f62024-04-10T10:54:04ZengAgroprint TimisoaraScientific Papers Animal Science and Biotechnologies1841-93642344-45762023-09-015111331331030Antimicrobial Activity and Resistance of Microorganisms Isolated from Honey BeesMiroslava Kačániová0Jaroslav Gasper1Margarita Terentjeva2Simona Kunová3Maciej Kluz4Pawel Hanus5Czeslaw Puchalski6Slovak University of Agriculture, Faculty of Biotechnology and Food Sciences, Nitra 949 76, Tr. A. Hlinku 2, Slovakia Slovak University of Agriculture, Faculty of Biotechnology and Food Sciences, Nitra 949 76, Tr. A. Hlinku 2, Slovakia Latvia University of Life Sciences and Technologies, Faculty of Veterinary Medicine, Institute of Food and Environmental Hygiene, Jelgava, LV-3004, K. Helmaņa iela 8, LatviaSlovak University of Agriculture, Faculty of Biotechnology and Food Sciences, Nitra 949 76, Tr. A. Hlinku 2, Slovakia University of Rzeszow, Faculty of Biology and Agriculture, 35-601 Rzeszow, Zelwerowicza St. 4, PolandUniversity of Rzeszow, Faculty of Biology and Agriculture, 35-601 Rzeszow, Zelwerowicza St. 4, PolandUniversity of Rzeszow, Faculty of Biology and Agriculture, 35-601 Rzeszow, Zelwerowicza St. 4, PolandKnowledge of composition of bee gut microbiota is important to ensure balanced gut microbiota and improve the health status of bees. The aim of the present study was to detect the microorganisms in gut of honey bee and to study their antimicrobial resistance and antimicrobial activity against three essential oils. Content of bees gut was studied with microbiological methods, and isolates were confirmed with MALDI TOF mass spectrometry. Isolated species were tested for antibiotic resistance to ciprofloxacin, levofloxacin, amikacin and gentamicin. For antimicrobial activity, essential oils of Abies alba, Pinus mugo and Pinus sylvestris were used. Gram-positive bacteria Bacillus oleronius, Micrococcus luteus, Staphylococcus epidermidis and three Gram-negative bacteria Pseudomonas oryzihabitans, Serratia fonticola, Serratia marcescens were isolated from bees gut. Antibiotic resistance was found in Gram-negative bacteria, while the Gram-positive bacteria were sensitive to all antibiotic tested. The best antibacterial activity of essential oils found against Gram-positive bacterial strains. Essential oils can be a potential sources of compounds with antimicrobial activity on Gram-positive isolates of bees gut.https://spasb.ro/index.php/public_html/article/view/1030beesantibiotic resistanceessential oilsmicroflora |
| spellingShingle | Miroslava Kačániová Jaroslav Gasper Margarita Terentjeva Simona Kunová Maciej Kluz Pawel Hanus Czeslaw Puchalski Antimicrobial Activity and Resistance of Microorganisms Isolated from Honey Bees Scientific Papers Animal Science and Biotechnologies bees antibiotic resistance essential oils microflora |
| title | Antimicrobial Activity and Resistance of Microorganisms Isolated from Honey Bees |
| title_full | Antimicrobial Activity and Resistance of Microorganisms Isolated from Honey Bees |
| title_fullStr | Antimicrobial Activity and Resistance of Microorganisms Isolated from Honey Bees |
| title_full_unstemmed | Antimicrobial Activity and Resistance of Microorganisms Isolated from Honey Bees |
| title_short | Antimicrobial Activity and Resistance of Microorganisms Isolated from Honey Bees |
| title_sort | antimicrobial activity and resistance of microorganisms isolated from honey bees |
| topic | bees antibiotic resistance essential oils microflora |
| url | https://spasb.ro/index.php/public_html/article/view/1030 |
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