The Anti-Cancer Effect of <em>Mangifera indica</em> L. Peel Extract is Associated to γH2AX-mediated Apoptosis in Colon Cancer Cells
Ethanolic extracts from <i>Mangifera indica</i> L. have been proved to possess anti-tumor properties in many cancer systems. However, although most effects have been demonstrated with fruit pulp extract, the underlying molecular mechanisms of mango peel are still unclear. This study was...
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| Format: | Article |
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MDPI AG
2019-09-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/8/10/422 |
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| author | Marianna Lauricella Valentina Lo Galbo Cesare Cernigliaro Antonella Maggio Antonio Palumbo Piccionello Giuseppe Calvaruso Daniela Carlisi Sonia Emanuele Michela Giuliano Antonella D’Anneo |
| author_facet | Marianna Lauricella Valentina Lo Galbo Cesare Cernigliaro Antonella Maggio Antonio Palumbo Piccionello Giuseppe Calvaruso Daniela Carlisi Sonia Emanuele Michela Giuliano Antonella D’Anneo |
| author_sort | Marianna Lauricella |
| collection | DOAJ |
| description | Ethanolic extracts from <i>Mangifera indica</i> L. have been proved to possess anti-tumor properties in many cancer systems. However, although most effects have been demonstrated with fruit pulp extract, the underlying molecular mechanisms of mango peel are still unclear. This study was designed to explore the effects of mango peel extract (MPE) on colon cancer cell lines. MPE affected cell viability and inhibited the colony formation trend of tumor cells, while no effects were observed in human dermal fibroblasts used as a non-cancerous cell line model. These events were a consequence of the induction of apoptosis associated to reactive oxygen species (ROS) production, activation of players of the oxidative response such as JNK and ERK1/2, and the increase in Nrf2 and manganese superoxide dismutase (MnSOD). Significantly, mango peel-activated stress triggered a DNA damage response evidenced by the precocious phosphorylation of histone 2AX (γH2AX), as well as phosphorylated Ataxia telangiectasia-mutated (ATM) kinase and p53 upregulation. Mango peel extract was also characterized, and HPLC/MS (High Performance Liquid Chromatography/Mass Spectrometry) analysis unveiled the presence of some phenolic compounds that could be responsible for the anti-cancer effects. Collectively, these findings point out the importance of the genotoxic stress signaling pathway mediated by γH2AX in targeting colon tumor cells to apoptosis. |
| first_indexed | 2024-03-12T07:44:57Z |
| format | Article |
| id | doaj.art-60cae61cc94e41ffa97af36a7b313183 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-12T07:44:57Z |
| publishDate | 2019-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-60cae61cc94e41ffa97af36a7b3131832023-09-02T21:05:00ZengMDPI AGAntioxidants2076-39212019-09-0181042210.3390/antiox8100422antiox8100422The Anti-Cancer Effect of <em>Mangifera indica</em> L. Peel Extract is Associated to γH2AX-mediated Apoptosis in Colon Cancer CellsMarianna Lauricella0Valentina Lo Galbo1Cesare Cernigliaro2Antonella Maggio3Antonio Palumbo Piccionello4Giuseppe Calvaruso5Daniela Carlisi6Sonia Emanuele7Michela Giuliano8Antonella D’Anneo9Department of Biomedicine, Neurosciences and Advanced Diagnostics (BIND), Institute of Biochemistry, University of Palermo, 90127 Palermo, ItalyLaboratory of Biochemistry, Department of Biological, Chemical and Pharmaceutical Sciences and Technologies (STEBICEF), University of Palermo, 90127 Palermo, ItalyDepartment of Biomedicine, Neurosciences and Advanced Diagnostics (BIND), Institute of Biochemistry, University of Palermo, 90127 Palermo, ItalyDepartment of Biological, Chemical and Pharmaceutical Sciences and Technologies (STEBICEF), Section of Chemistry, University of Palermo, 90128 Palermo, ItalyDepartment of Biological, Chemical and Pharmaceutical Sciences and Technologies (STEBICEF), Section of Chemistry, University of Palermo, 90128 Palermo, ItalyLaboratory of Biochemistry, Department of Biological, Chemical and Pharmaceutical Sciences and Technologies (STEBICEF), University of Palermo, 90127 Palermo, ItalyDepartment of Biomedicine, Neurosciences and Advanced Diagnostics (BIND), Institute of Biochemistry, University of Palermo, 90127 Palermo, ItalyDepartment of Biomedicine, Neurosciences and Advanced Diagnostics (BIND), Institute of Biochemistry, University of Palermo, 90127 Palermo, ItalyLaboratory of Biochemistry, Department of Biological, Chemical and Pharmaceutical Sciences and Technologies (STEBICEF), University of Palermo, 90127 Palermo, ItalyLaboratory of Biochemistry, Department of Biological, Chemical and Pharmaceutical Sciences and Technologies (STEBICEF), University of Palermo, 90127 Palermo, ItalyEthanolic extracts from <i>Mangifera indica</i> L. have been proved to possess anti-tumor properties in many cancer systems. However, although most effects have been demonstrated with fruit pulp extract, the underlying molecular mechanisms of mango peel are still unclear. This study was designed to explore the effects of mango peel extract (MPE) on colon cancer cell lines. MPE affected cell viability and inhibited the colony formation trend of tumor cells, while no effects were observed in human dermal fibroblasts used as a non-cancerous cell line model. These events were a consequence of the induction of apoptosis associated to reactive oxygen species (ROS) production, activation of players of the oxidative response such as JNK and ERK1/2, and the increase in Nrf2 and manganese superoxide dismutase (MnSOD). Significantly, mango peel-activated stress triggered a DNA damage response evidenced by the precocious phosphorylation of histone 2AX (γH2AX), as well as phosphorylated Ataxia telangiectasia-mutated (ATM) kinase and p53 upregulation. Mango peel extract was also characterized, and HPLC/MS (High Performance Liquid Chromatography/Mass Spectrometry) analysis unveiled the presence of some phenolic compounds that could be responsible for the anti-cancer effects. Collectively, these findings point out the importance of the genotoxic stress signaling pathway mediated by γH2AX in targeting colon tumor cells to apoptosis.https://www.mdpi.com/2076-3921/8/10/422mangoapoptosisreactive oxygen speciesγH2AXcolon cancer cells |
| spellingShingle | Marianna Lauricella Valentina Lo Galbo Cesare Cernigliaro Antonella Maggio Antonio Palumbo Piccionello Giuseppe Calvaruso Daniela Carlisi Sonia Emanuele Michela Giuliano Antonella D’Anneo The Anti-Cancer Effect of <em>Mangifera indica</em> L. Peel Extract is Associated to γH2AX-mediated Apoptosis in Colon Cancer Cells Antioxidants mango apoptosis reactive oxygen species γH2AX colon cancer cells |
| title | The Anti-Cancer Effect of <em>Mangifera indica</em> L. Peel Extract is Associated to γH2AX-mediated Apoptosis in Colon Cancer Cells |
| title_full | The Anti-Cancer Effect of <em>Mangifera indica</em> L. Peel Extract is Associated to γH2AX-mediated Apoptosis in Colon Cancer Cells |
| title_fullStr | The Anti-Cancer Effect of <em>Mangifera indica</em> L. Peel Extract is Associated to γH2AX-mediated Apoptosis in Colon Cancer Cells |
| title_full_unstemmed | The Anti-Cancer Effect of <em>Mangifera indica</em> L. Peel Extract is Associated to γH2AX-mediated Apoptosis in Colon Cancer Cells |
| title_short | The Anti-Cancer Effect of <em>Mangifera indica</em> L. Peel Extract is Associated to γH2AX-mediated Apoptosis in Colon Cancer Cells |
| title_sort | anti cancer effect of em mangifera indica em l peel extract is associated to γh2ax mediated apoptosis in colon cancer cells |
| topic | mango apoptosis reactive oxygen species γH2AX colon cancer cells |
| url | https://www.mdpi.com/2076-3921/8/10/422 |
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