Gold Nanorods Coated with Multilayer Polyelectrolyte as Intracellular delivery Vector of Antisense Oligonucleotides
A novel functionaliztion of gold nanorods (GNRs) intracellular delivery system was prepared via electrostatically layer-by-layer assemblying, in which multilayer polyelectrolyte-coated GNRs were used as vector and antisense oligodeoxynucleotides (ASODNs) as therapeutic gene. The as-prepared positive...
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| Format: | Article |
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Tsinghua University Press
2010-03-01
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| Series: | Nano Biomedicine and Engineering |
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| Online Access: | https://www.sciopen.com/article/10.5101/nbe.v2i1.p15-23 |
| _version_ | 1797673787696611328 |
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| author | Shouhui Chen Yuxuan Ji Qiong Lian Yanli Wen Hebai Shen Nengqin Jia |
| author_facet | Shouhui Chen Yuxuan Ji Qiong Lian Yanli Wen Hebai Shen Nengqin Jia |
| author_sort | Shouhui Chen |
| collection | DOAJ |
| description | A novel functionaliztion of gold nanorods (GNRs) intracellular delivery system was prepared via electrostatically layer-by-layer assemblying, in which multilayer polyelectrolyte-coated GNRs were used as vector and antisense oligodeoxynucleotides (ASODNs) as therapeutic gene. The as-prepared positively charged GNRs were firstly modified with two successive polyelectrolyte layers of the negatively charged poly(sodium-4-styrenesulfonate) (PSS) and positively charged polyenthylenimine (PEI) in order to greatly improve the biocompatibility of these cetyltrimethylammonium bromide(CTAB)-coated GNRs and facilitate further biofunctionalization. The multilayered polyelectrolyte functionalized GNRs were characterized by UV-vis spectroscopy, transmission electron microscopy (TEM) and MTT assay. The polyelectrolytes-coated GNRs were then used as vector for ASODNs. Electrochemical impedance spectroscopy(EIS) was employed to confirm the formation of the GNRs-ASODNs conjugates through electrostatic interaction. Furthermore, cellular uptake and delivery efficiency of the GNRs-ASODNs conjugates as well as cellular apoptosis induced by the ASODNs transfected with gold nanorods were investigated by confocal microscopy and flow cytometry, exhibiting efficient intracellular delivery and improved antitumor activity of ASODNs by the polyelectrolytes-coated GNRs carriers. |
| first_indexed | 2024-03-11T21:49:29Z |
| format | Article |
| id | doaj.art-60cc2cda3991480e99735d3f482ecca5 |
| institution | Directory Open Access Journal |
| issn | 2150-5578 |
| language | English |
| last_indexed | 2024-03-11T21:49:29Z |
| publishDate | 2010-03-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Nano Biomedicine and Engineering |
| spelling | doaj.art-60cc2cda3991480e99735d3f482ecca52023-09-26T09:03:15ZengTsinghua University PressNano Biomedicine and Engineering2150-55782010-03-0121152310.5101/nbe.v2i1.p15-23Gold Nanorods Coated with Multilayer Polyelectrolyte as Intracellular delivery Vector of Antisense OligonucleotidesShouhui Chen0Yuxuan Ji1Qiong Lian2Yanli Wen3Hebai Shen4Nengqin Jia5Department of Chemistry, College of Life and Environmental Science, Shanghai Normal University, Shanghai 200234, ChinaDepartment of Chemistry, College of Life and Environmental Science, Shanghai Normal University, Shanghai 200234, ChinaDepartment of Chemistry, College of Life and Environmental Science, Shanghai Normal University, Shanghai 200234, ChinaDepartment of Chemistry, College of Life and Environmental Science, Shanghai Normal University, Shanghai 200234, ChinaDepartment of Chemistry, College of Life and Environmental Science, Shanghai Normal University, Shanghai 200234, ChinaDepartment of Chemistry, College of Life and Environmental Science, Shanghai Normal University, Shanghai 200234, ChinaA novel functionaliztion of gold nanorods (GNRs) intracellular delivery system was prepared via electrostatically layer-by-layer assemblying, in which multilayer polyelectrolyte-coated GNRs were used as vector and antisense oligodeoxynucleotides (ASODNs) as therapeutic gene. The as-prepared positively charged GNRs were firstly modified with two successive polyelectrolyte layers of the negatively charged poly(sodium-4-styrenesulfonate) (PSS) and positively charged polyenthylenimine (PEI) in order to greatly improve the biocompatibility of these cetyltrimethylammonium bromide(CTAB)-coated GNRs and facilitate further biofunctionalization. The multilayered polyelectrolyte functionalized GNRs were characterized by UV-vis spectroscopy, transmission electron microscopy (TEM) and MTT assay. The polyelectrolytes-coated GNRs were then used as vector for ASODNs. Electrochemical impedance spectroscopy(EIS) was employed to confirm the formation of the GNRs-ASODNs conjugates through electrostatic interaction. Furthermore, cellular uptake and delivery efficiency of the GNRs-ASODNs conjugates as well as cellular apoptosis induced by the ASODNs transfected with gold nanorods were investigated by confocal microscopy and flow cytometry, exhibiting efficient intracellular delivery and improved antitumor activity of ASODNs by the polyelectrolytes-coated GNRs carriers.https://www.sciopen.com/article/10.5101/nbe.v2i1.p15-23gold nanorodspolyelectrolytesantisense oligodeoxynucleotidesbiocompatibilityintracellular delivery vector |
| spellingShingle | Shouhui Chen Yuxuan Ji Qiong Lian Yanli Wen Hebai Shen Nengqin Jia Gold Nanorods Coated with Multilayer Polyelectrolyte as Intracellular delivery Vector of Antisense Oligonucleotides Nano Biomedicine and Engineering gold nanorods polyelectrolytes antisense oligodeoxynucleotides biocompatibility intracellular delivery vector |
| title | Gold Nanorods Coated with Multilayer Polyelectrolyte as Intracellular delivery Vector of Antisense Oligonucleotides |
| title_full | Gold Nanorods Coated with Multilayer Polyelectrolyte as Intracellular delivery Vector of Antisense Oligonucleotides |
| title_fullStr | Gold Nanorods Coated with Multilayer Polyelectrolyte as Intracellular delivery Vector of Antisense Oligonucleotides |
| title_full_unstemmed | Gold Nanorods Coated with Multilayer Polyelectrolyte as Intracellular delivery Vector of Antisense Oligonucleotides |
| title_short | Gold Nanorods Coated with Multilayer Polyelectrolyte as Intracellular delivery Vector of Antisense Oligonucleotides |
| title_sort | gold nanorods coated with multilayer polyelectrolyte as intracellular delivery vector of antisense oligonucleotides |
| topic | gold nanorods polyelectrolytes antisense oligodeoxynucleotides biocompatibility intracellular delivery vector |
| url | https://www.sciopen.com/article/10.5101/nbe.v2i1.p15-23 |
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