Modulating phase for adaptive optics and PSF shaping in bio-imaging: Requirements & development of a new deformable mirror tailored to microscopy
Modern bio-imaging techniques such as light-sheet, multiphoton and PALM/STORM are now aiming to image more complex biological samples at larger depth and therefore face larger-amplitude and more complex aberrations. We provide an analysis of key requirements driving optimal implementation of adaptiv...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
EDP Sciences
2023-01-01
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| Series: | EPJ Web of Conferences |
| Online Access: | https://www.epj-conferences.org/articles/epjconf/pdf/2023/13/epjconf_eosam2023_02004.pdf |
| _version_ | 1797634804257128448 |
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| author | Harms Fabrice Veilly Cynthia Jasaitis Audrius Dovillaire Guillaume Levecq Xavier |
| author_facet | Harms Fabrice Veilly Cynthia Jasaitis Audrius Dovillaire Guillaume Levecq Xavier |
| author_sort | Harms Fabrice |
| collection | DOAJ |
| description | Modern bio-imaging techniques such as light-sheet, multiphoton and PALM/STORM are now aiming to image more complex biological samples at larger depth and therefore face larger-amplitude and more complex aberrations. We provide an analysis of key requirements driving optimal implementation of adaptive optics (AO) in microscopy, with a focus on wavefront modulators. We show that some specifications of wavefront modulators such as linearity, hysteresis or actuators performance & layout can end up to better AO performance in microscopy systems, when specifically optimized for such use. We then provide design details and characterization results of a newly developed deformable mirror, and report on experimental images obtained from AO-enhanced microscopes based on the device, for several modalities such as light-sheet, multiphoton or super-resolution single molecule localization systems. Finally, we provide recommendations on how to define the right set of AO components, algorithms and overall method depending on modality, instrument and sample constraints. |
| first_indexed | 2024-03-11T12:13:37Z |
| format | Article |
| id | doaj.art-60d16402ff4c4d4cb5751794151a425a |
| institution | Directory Open Access Journal |
| issn | 2100-014X |
| language | English |
| last_indexed | 2024-03-11T12:13:37Z |
| publishDate | 2023-01-01 |
| publisher | EDP Sciences |
| record_format | Article |
| series | EPJ Web of Conferences |
| spelling | doaj.art-60d16402ff4c4d4cb5751794151a425a2023-11-07T10:20:47ZengEDP SciencesEPJ Web of Conferences2100-014X2023-01-012870200410.1051/epjconf/202328702004epjconf_eosam2023_02004Modulating phase for adaptive optics and PSF shaping in bio-imaging: Requirements & development of a new deformable mirror tailored to microscopyHarms Fabrice0Veilly Cynthia1Jasaitis Audrius2Dovillaire Guillaume3Levecq Xavier4Imagine OpticImagine OpticImagine OpticImagine OpticImagine OpticModern bio-imaging techniques such as light-sheet, multiphoton and PALM/STORM are now aiming to image more complex biological samples at larger depth and therefore face larger-amplitude and more complex aberrations. We provide an analysis of key requirements driving optimal implementation of adaptive optics (AO) in microscopy, with a focus on wavefront modulators. We show that some specifications of wavefront modulators such as linearity, hysteresis or actuators performance & layout can end up to better AO performance in microscopy systems, when specifically optimized for such use. We then provide design details and characterization results of a newly developed deformable mirror, and report on experimental images obtained from AO-enhanced microscopes based on the device, for several modalities such as light-sheet, multiphoton or super-resolution single molecule localization systems. Finally, we provide recommendations on how to define the right set of AO components, algorithms and overall method depending on modality, instrument and sample constraints.https://www.epj-conferences.org/articles/epjconf/pdf/2023/13/epjconf_eosam2023_02004.pdf |
| spellingShingle | Harms Fabrice Veilly Cynthia Jasaitis Audrius Dovillaire Guillaume Levecq Xavier Modulating phase for adaptive optics and PSF shaping in bio-imaging: Requirements & development of a new deformable mirror tailored to microscopy EPJ Web of Conferences |
| title | Modulating phase for adaptive optics and PSF shaping in bio-imaging: Requirements & development of a new deformable mirror tailored to microscopy |
| title_full | Modulating phase for adaptive optics and PSF shaping in bio-imaging: Requirements & development of a new deformable mirror tailored to microscopy |
| title_fullStr | Modulating phase for adaptive optics and PSF shaping in bio-imaging: Requirements & development of a new deformable mirror tailored to microscopy |
| title_full_unstemmed | Modulating phase for adaptive optics and PSF shaping in bio-imaging: Requirements & development of a new deformable mirror tailored to microscopy |
| title_short | Modulating phase for adaptive optics and PSF shaping in bio-imaging: Requirements & development of a new deformable mirror tailored to microscopy |
| title_sort | modulating phase for adaptive optics and psf shaping in bio imaging requirements development of a new deformable mirror tailored to microscopy |
| url | https://www.epj-conferences.org/articles/epjconf/pdf/2023/13/epjconf_eosam2023_02004.pdf |
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