Osteogenic Effect of Rabbit Periosteum-Derived Precursor Cells Co-Induced by Electric Stimulation and Adipose-Derived Stem Cells in a 3D Co-Culture System
Periosteum-derived progenitor cells (PDPCs) are highly promising cell sources for bone fracture healing because of their stem cell-like multipotency to undergo osteogenesis and chondrogenesis. Both externally physical stimulation and internally biochemical signal were reported to enhance osteogenic...
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| Format: | Article |
| Language: | English |
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IEEE
2021-01-01
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| Series: | IEEE Open Journal of Nanotechnology |
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| Online Access: | https://ieeexplore.ieee.org/document/9633183/ |
| _version_ | 1818331702847602688 |
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| author | Alvin Chen Yun-Wen Tong Chih-Hao Chiu Kin Fong Lei |
| author_facet | Alvin Chen Yun-Wen Tong Chih-Hao Chiu Kin Fong Lei |
| author_sort | Alvin Chen |
| collection | DOAJ |
| description | Periosteum-derived progenitor cells (PDPCs) are highly promising cell sources for bone fracture healing because of their stem cell-like multipotency to undergo osteogenesis and chondrogenesis. Both externally physical stimulation and internally biochemical signal were reported to enhance osteogenic differentiation of bone tissues. Electric stimulation (ES) could trigger the differentiation of stem cells, like mesenchymal stem cells (MSCs) and adipose-derived stem cells (ADSCs). But the effect is still unclear on PDPCs. In order to investigate the differentiation ability of PDPCs co-induced by ES and ADSCs, a biomimetic 3-dimensional (3D) co-culture system was developed for providing ES and co-culturing with ADSCs. Gene expression was studied after a 3-day culture course. From our results, osteogenic differentiation of PDPCs was significantly activated under the ES of 0.7 V/cm, 80 kHz, and 3 hrs/day. Moreover, co-culturing with ADSCs during the ES treatment was found to have synergistic effect of osteogenic differentiation. In addition, chondrogenic differentiation was shown when the PDPCs were cultured for a long culture course. In summary, osteogenic differentiation of PDPCs was shown to be co-induced by ES and ADSCs. This study provides significant insights of the PDPC therapy for bone tissue regeneration. |
| first_indexed | 2024-12-13T13:24:03Z |
| format | Article |
| id | doaj.art-60d18325510f46879203ca8abbc68591 |
| institution | Directory Open Access Journal |
| issn | 2644-1292 |
| language | English |
| last_indexed | 2024-12-13T13:24:03Z |
| publishDate | 2021-01-01 |
| publisher | IEEE |
| record_format | Article |
| series | IEEE Open Journal of Nanotechnology |
| spelling | doaj.art-60d18325510f46879203ca8abbc685912022-12-21T23:44:21ZengIEEEIEEE Open Journal of Nanotechnology2644-12922021-01-01215316010.1109/OJNANO.2021.31316539633183Osteogenic Effect of Rabbit Periosteum-Derived Precursor Cells Co-Induced by Electric Stimulation and Adipose-Derived Stem Cells in a 3D Co-Culture SystemAlvin Chen0Yun-Wen Tong1Chih-Hao Chiu2https://orcid.org/0000-0001-9415-5115Kin Fong Lei3https://orcid.org/0000-0002-5356-2221Department of Orthopedic Surgery, Bone and Joint Research Center, and Comprehensive Sports Medicine Center, Chang Gung Memorial Hospital, Linkou, TaiwanDepartment of Orthopedic Surgery, Chang Gung Memorial Hospital, Linkou, TaiwanDepartment of Orthopedic Surgery, Chang Gung Memorial Hospital, Taoyuan, TaiwanGraduate Institute of Biomedical Engineering, Chang Gung University, Taoyuan, TaiwanPeriosteum-derived progenitor cells (PDPCs) are highly promising cell sources for bone fracture healing because of their stem cell-like multipotency to undergo osteogenesis and chondrogenesis. Both externally physical stimulation and internally biochemical signal were reported to enhance osteogenic differentiation of bone tissues. Electric stimulation (ES) could trigger the differentiation of stem cells, like mesenchymal stem cells (MSCs) and adipose-derived stem cells (ADSCs). But the effect is still unclear on PDPCs. In order to investigate the differentiation ability of PDPCs co-induced by ES and ADSCs, a biomimetic 3-dimensional (3D) co-culture system was developed for providing ES and co-culturing with ADSCs. Gene expression was studied after a 3-day culture course. From our results, osteogenic differentiation of PDPCs was significantly activated under the ES of 0.7 V/cm, 80 kHz, and 3 hrs/day. Moreover, co-culturing with ADSCs during the ES treatment was found to have synergistic effect of osteogenic differentiation. In addition, chondrogenic differentiation was shown when the PDPCs were cultured for a long culture course. In summary, osteogenic differentiation of PDPCs was shown to be co-induced by ES and ADSCs. This study provides significant insights of the PDPC therapy for bone tissue regeneration.https://ieeexplore.ieee.org/document/9633183/Periosteum-derived progenitor cellsadipose-derived stem cellsosteogenesis3D Co-cultureelectric stimulationorthopedics |
| spellingShingle | Alvin Chen Yun-Wen Tong Chih-Hao Chiu Kin Fong Lei Osteogenic Effect of Rabbit Periosteum-Derived Precursor Cells Co-Induced by Electric Stimulation and Adipose-Derived Stem Cells in a 3D Co-Culture System IEEE Open Journal of Nanotechnology Periosteum-derived progenitor cells adipose-derived stem cells osteogenesis 3D Co-culture electric stimulation orthopedics |
| title | Osteogenic Effect of Rabbit Periosteum-Derived Precursor Cells Co-Induced by Electric Stimulation and Adipose-Derived Stem Cells in a 3D Co-Culture System |
| title_full | Osteogenic Effect of Rabbit Periosteum-Derived Precursor Cells Co-Induced by Electric Stimulation and Adipose-Derived Stem Cells in a 3D Co-Culture System |
| title_fullStr | Osteogenic Effect of Rabbit Periosteum-Derived Precursor Cells Co-Induced by Electric Stimulation and Adipose-Derived Stem Cells in a 3D Co-Culture System |
| title_full_unstemmed | Osteogenic Effect of Rabbit Periosteum-Derived Precursor Cells Co-Induced by Electric Stimulation and Adipose-Derived Stem Cells in a 3D Co-Culture System |
| title_short | Osteogenic Effect of Rabbit Periosteum-Derived Precursor Cells Co-Induced by Electric Stimulation and Adipose-Derived Stem Cells in a 3D Co-Culture System |
| title_sort | osteogenic effect of rabbit periosteum derived precursor cells co induced by electric stimulation and adipose derived stem cells in a 3d co culture system |
| topic | Periosteum-derived progenitor cells adipose-derived stem cells osteogenesis 3D Co-culture electric stimulation orthopedics |
| url | https://ieeexplore.ieee.org/document/9633183/ |
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