Pre-Pandemic Cross-Reactive Immunity against SARS-CoV-2 among Siberian Populations
In December 2019, a new coronavirus, SARS-CoV-2, was found to in Wuhan, China. Cases of infection were subsequently detected in other countries in a short period of time, resulting in the declaration of the COVID-19 pandemic by the World Health Organization (WHO) on 11 March 2020. Questions about th...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-12-01
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| Series: | Antibodies |
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| Online Access: | https://www.mdpi.com/2073-4468/12/4/82 |
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| author | Olga N. Shaprova Daniil V. Shanshin Evgeniia A. Kolosova Sophia S. Borisevich Artem A. Soroka Iuliia A. Merkuleva Artem O. Nikitin Ekaterina A. Volosnikova Nikita D. Ushkalenko Anna V. Zaykovskaya Oleg V. Pyankov Svetlana A. Elchaninova Dmitry N. Shcherbakov Tatiana N. Ilyicheva |
| author_facet | Olga N. Shaprova Daniil V. Shanshin Evgeniia A. Kolosova Sophia S. Borisevich Artem A. Soroka Iuliia A. Merkuleva Artem O. Nikitin Ekaterina A. Volosnikova Nikita D. Ushkalenko Anna V. Zaykovskaya Oleg V. Pyankov Svetlana A. Elchaninova Dmitry N. Shcherbakov Tatiana N. Ilyicheva |
| author_sort | Olga N. Shaprova |
| collection | DOAJ |
| description | In December 2019, a new coronavirus, SARS-CoV-2, was found to in Wuhan, China. Cases of infection were subsequently detected in other countries in a short period of time, resulting in the declaration of the COVID-19 pandemic by the World Health Organization (WHO) on 11 March 2020. Questions about the impact of herd immunity of pre-existing immune reactivity to SARS-CoV-2 on COVID-19 severity, associated with the immunity to seasonal manifestation, are still to be resolved and may be useful for understanding some processes that precede the emergence of a pandemic virus. Perhaps this will contribute to understanding some of the processes that precede the emergence of a pandemic virus. We assessed the specificity and virus-neutralizing capacity of antibodies reacting with the nucleocapsid and spike proteins of SARS-CoV-2 in a set of serum samples collected in October and November 2019, before the first COVID-19 cases were documented in this region. Blood serum samples from 799 residents of several regions of Siberia, Russia, (the Altai Territory, Irkutsk, Kemerovo and Novosibirsk regions, the Republic of Altai, Buryatia, and Khakassia) were analyzed. Sera of non-infected donors were collected within a study of seasonal influenza in the Russian Federation. The sample collection sites were located near the flyways and breeding grounds of wild waterfowl. The performance of enzyme-linked immunosorbent assay (ELISA) for the collected sera included the usage of recombinant SARS-CoV-2 protein antigens: full-length nucleocapsid protein (CoVN), receptor binding domain (RBD) of S-protein and infection fragment of the S protein (S5-6). There were 183 (22.9%) sera reactive to the S5-6, 270 (33.8%) sera corresponding to the full-length N protein and 128 (16.2%) sera simultaneously reactive to both these proteins. Only 5 out of 799 sera had IgG antibodies reactive to the RBD. None of the sera exhibited neutralizing activity against the nCoV/Victoria/1/2020 SARS-CoV-2 strain in Vero E6 cell culture. The data obtained in this study suggest that some of the population of the analyzed regions of Russia had cross-reactive humoral immunity against SARS-CoV-2 before the COVID-19 pandemic started. Moreover, among individuals from relatively isolated regions, there were significantly fewer reliably cross-reactive sera. The possible significance of these data and impact of cross-immunity to SARS-CoV-2 on the prevalence and mortality of COVID-19 needs further assessment. |
| first_indexed | 2024-03-08T21:02:33Z |
| format | Article |
| id | doaj.art-60d5945dbe5242978ff1eea22b18388f |
| institution | Directory Open Access Journal |
| issn | 2073-4468 |
| language | English |
| last_indexed | 2024-03-08T21:02:33Z |
| publishDate | 2023-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibodies |
| spelling | doaj.art-60d5945dbe5242978ff1eea22b18388f2023-12-22T13:48:13ZengMDPI AGAntibodies2073-44682023-12-011248210.3390/antib12040082Pre-Pandemic Cross-Reactive Immunity against SARS-CoV-2 among Siberian PopulationsOlga N. Shaprova0Daniil V. Shanshin1Evgeniia A. Kolosova2Sophia S. Borisevich3Artem A. Soroka4Iuliia A. Merkuleva5Artem O. Nikitin6Ekaterina A. Volosnikova7Nikita D. Ushkalenko8Anna V. Zaykovskaya9Oleg V. Pyankov10Svetlana A. Elchaninova11Dmitry N. Shcherbakov12Tatiana N. Ilyicheva13State Research Center of Virology and Biotechnology VECTOR, Rospotrebnadzor, 630559 Koltsovo, RussiaState Research Center of Virology and Biotechnology VECTOR, Rospotrebnadzor, 630559 Koltsovo, RussiaState Research Center of Virology and Biotechnology VECTOR, Rospotrebnadzor, 630559 Koltsovo, RussiaLaboratory of Chemical Physics, Ufa Institute of Chemistry Ufa Federal Research Center, 450078 Ufa, RussiaInstitute of Intelligent Cybernetic Systems, National Research Nuclear University MEPhI (Moscow Engineering Physics Institute), 115409 Moscow, RussiaState Research Center of Virology and Biotechnology VECTOR, Rospotrebnadzor, 630559 Koltsovo, RussiaState Research Center of Virology and Biotechnology VECTOR, Rospotrebnadzor, 630559 Koltsovo, RussiaState Research Center of Virology and Biotechnology VECTOR, Rospotrebnadzor, 630559 Koltsovo, RussiaState Research Center of Virology and Biotechnology VECTOR, Rospotrebnadzor, 630559 Koltsovo, RussiaState Research Center of Virology and Biotechnology VECTOR, Rospotrebnadzor, 630559 Koltsovo, RussiaState Research Center of Virology and Biotechnology VECTOR, Rospotrebnadzor, 630559 Koltsovo, RussiaDepartment of Biochemistry and Clinical Laboratory Diagnostics, Altai State Medical University, 656038 Barnaul, RussiaState Research Center of Virology and Biotechnology VECTOR, Rospotrebnadzor, 630559 Koltsovo, RussiaState Research Center of Virology and Biotechnology VECTOR, Rospotrebnadzor, 630559 Koltsovo, RussiaIn December 2019, a new coronavirus, SARS-CoV-2, was found to in Wuhan, China. Cases of infection were subsequently detected in other countries in a short period of time, resulting in the declaration of the COVID-19 pandemic by the World Health Organization (WHO) on 11 March 2020. Questions about the impact of herd immunity of pre-existing immune reactivity to SARS-CoV-2 on COVID-19 severity, associated with the immunity to seasonal manifestation, are still to be resolved and may be useful for understanding some processes that precede the emergence of a pandemic virus. Perhaps this will contribute to understanding some of the processes that precede the emergence of a pandemic virus. We assessed the specificity and virus-neutralizing capacity of antibodies reacting with the nucleocapsid and spike proteins of SARS-CoV-2 in a set of serum samples collected in October and November 2019, before the first COVID-19 cases were documented in this region. Blood serum samples from 799 residents of several regions of Siberia, Russia, (the Altai Territory, Irkutsk, Kemerovo and Novosibirsk regions, the Republic of Altai, Buryatia, and Khakassia) were analyzed. Sera of non-infected donors were collected within a study of seasonal influenza in the Russian Federation. The sample collection sites were located near the flyways and breeding grounds of wild waterfowl. The performance of enzyme-linked immunosorbent assay (ELISA) for the collected sera included the usage of recombinant SARS-CoV-2 protein antigens: full-length nucleocapsid protein (CoVN), receptor binding domain (RBD) of S-protein and infection fragment of the S protein (S5-6). There were 183 (22.9%) sera reactive to the S5-6, 270 (33.8%) sera corresponding to the full-length N protein and 128 (16.2%) sera simultaneously reactive to both these proteins. Only 5 out of 799 sera had IgG antibodies reactive to the RBD. None of the sera exhibited neutralizing activity against the nCoV/Victoria/1/2020 SARS-CoV-2 strain in Vero E6 cell culture. The data obtained in this study suggest that some of the population of the analyzed regions of Russia had cross-reactive humoral immunity against SARS-CoV-2 before the COVID-19 pandemic started. Moreover, among individuals from relatively isolated regions, there were significantly fewer reliably cross-reactive sera. The possible significance of these data and impact of cross-immunity to SARS-CoV-2 on the prevalence and mortality of COVID-19 needs further assessment.https://www.mdpi.com/2073-4468/12/4/82SARS-CoV-2antibodiesIgGcoronaviruspre-existing immunity |
| spellingShingle | Olga N. Shaprova Daniil V. Shanshin Evgeniia A. Kolosova Sophia S. Borisevich Artem A. Soroka Iuliia A. Merkuleva Artem O. Nikitin Ekaterina A. Volosnikova Nikita D. Ushkalenko Anna V. Zaykovskaya Oleg V. Pyankov Svetlana A. Elchaninova Dmitry N. Shcherbakov Tatiana N. Ilyicheva Pre-Pandemic Cross-Reactive Immunity against SARS-CoV-2 among Siberian Populations Antibodies SARS-CoV-2 antibodies IgG coronavirus pre-existing immunity |
| title | Pre-Pandemic Cross-Reactive Immunity against SARS-CoV-2 among Siberian Populations |
| title_full | Pre-Pandemic Cross-Reactive Immunity against SARS-CoV-2 among Siberian Populations |
| title_fullStr | Pre-Pandemic Cross-Reactive Immunity against SARS-CoV-2 among Siberian Populations |
| title_full_unstemmed | Pre-Pandemic Cross-Reactive Immunity against SARS-CoV-2 among Siberian Populations |
| title_short | Pre-Pandemic Cross-Reactive Immunity against SARS-CoV-2 among Siberian Populations |
| title_sort | pre pandemic cross reactive immunity against sars cov 2 among siberian populations |
| topic | SARS-CoV-2 antibodies IgG coronavirus pre-existing immunity |
| url | https://www.mdpi.com/2073-4468/12/4/82 |
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