GRP78 protein metabolism in obese and diabetic rats: a study of its role in metabolic disorders
Abstract Objective This study aimed to compare and analyze the expression and significance of the GRP78 protein in cochlear cell injury induced by a high glucose and high-fat diet in obese and diabetic rats. Methods Male SD rats were randomly divided into two groups: normal (NC) and high-fat (HF) gr...
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| Format: | Article |
| Language: | English |
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BMC
2024-01-01
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| Series: | Diabetology & Metabolic Syndrome |
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| Online Access: | https://doi.org/10.1186/s13098-024-01255-6 |
| _version_ | 1827382065100750848 |
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| author | Kai Xi Hua-Ping Li Yue-Hui Wang Yang-Yang Li Lei Wang Miao-Miao Zhang Xi Zhang Bing-Wen Xing |
| author_facet | Kai Xi Hua-Ping Li Yue-Hui Wang Yang-Yang Li Lei Wang Miao-Miao Zhang Xi Zhang Bing-Wen Xing |
| author_sort | Kai Xi |
| collection | DOAJ |
| description | Abstract Objective This study aimed to compare and analyze the expression and significance of the GRP78 protein in cochlear cell injury induced by a high glucose and high-fat diet in obese and diabetic rats. Methods Male SD rats were randomly divided into two groups: normal (NC) and high-fat (HF) groups. The NC group was fed a standard diet for eight weeks, while the HF group received a high-glucose, high-fat diet. The HF group was further categorized into the obesity group (OB group) and the type II diabetes mellitus group (T2DM group). To induce a type II diabetes mellitus (T2DM) model, the T2DM group received an intraperitoneal injection of a small dose of STZ (45 mg/kg). After four weeks on the original diet, body weight, blood glucose, blood lipid levels, and auditory brainstem response (ABR) thresholds were measured. The cochlea was dissected, and its morphology was observed using HE staining. Immunohistochemistry and western blotting were utilized to examine the expression level of the GRP78 protein in the cochlea. Results (1) The ABR threshold demonstrated a statistically significant difference between the T2DM group and the OB group (P < 0.05), as well as between the OB group and the NC group (P < 0.05). (2) Based on morphological comparisons from HE-stained sections, the T2DM group exhibited the most significant alterations in the number of cells in the spiral ganglion, the organ of Corti, and the stria vascularis of the cochlea. (3) The expression level of the GRP78 protein in the cochlea was higher in the T2DM group compared to the OB group (P < 0.05) and higher in the OB group compared to the NC group (P < 0.05). Conclusion The findings indicate that the GRP78 protein plays a role in hearing loss caused by T2DM and hyperlipidemia. Moreover, T2DM is more likely than hyperlipidemia to be associated with hearing impairment. |
| first_indexed | 2024-03-08T14:14:07Z |
| format | Article |
| id | doaj.art-60d62748c03a4a6ea0edc6b31748db7f |
| institution | Directory Open Access Journal |
| issn | 1758-5996 |
| language | English |
| last_indexed | 2024-03-08T14:14:07Z |
| publishDate | 2024-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Diabetology & Metabolic Syndrome |
| spelling | doaj.art-60d62748c03a4a6ea0edc6b31748db7f2024-01-14T12:30:53ZengBMCDiabetology & Metabolic Syndrome1758-59962024-01-011611810.1186/s13098-024-01255-6GRP78 protein metabolism in obese and diabetic rats: a study of its role in metabolic disordersKai Xi0Hua-Ping Li1Yue-Hui Wang2Yang-Yang Li3Lei Wang4Miao-Miao Zhang5Xi Zhang6Bing-Wen Xing7The First Affiliated Hospital, and College of Clinical Medicine of Henan University of Science and TechnologyThe First Affiliated Hospital, and College of Clinical Medicine of Henan University of Science and TechnologyThe First Affiliated Hospital, and College of Clinical Medicine of Henan University of Science and TechnologyThe First Affiliated Hospital, and College of Clinical Medicine of Henan University of Science and TechnologyThe First Affiliated Hospital, and College of Clinical Medicine of Henan University of Science and TechnologyThe First Affiliated Hospital, and College of Clinical Medicine of Henan University of Science and TechnologyThe First Affiliated Hospital, and College of Clinical Medicine of Henan University of Science and TechnologyThe First Affiliated Hospital, and College of Clinical Medicine of Henan University of Science and TechnologyAbstract Objective This study aimed to compare and analyze the expression and significance of the GRP78 protein in cochlear cell injury induced by a high glucose and high-fat diet in obese and diabetic rats. Methods Male SD rats were randomly divided into two groups: normal (NC) and high-fat (HF) groups. The NC group was fed a standard diet for eight weeks, while the HF group received a high-glucose, high-fat diet. The HF group was further categorized into the obesity group (OB group) and the type II diabetes mellitus group (T2DM group). To induce a type II diabetes mellitus (T2DM) model, the T2DM group received an intraperitoneal injection of a small dose of STZ (45 mg/kg). After four weeks on the original diet, body weight, blood glucose, blood lipid levels, and auditory brainstem response (ABR) thresholds were measured. The cochlea was dissected, and its morphology was observed using HE staining. Immunohistochemistry and western blotting were utilized to examine the expression level of the GRP78 protein in the cochlea. Results (1) The ABR threshold demonstrated a statistically significant difference between the T2DM group and the OB group (P < 0.05), as well as between the OB group and the NC group (P < 0.05). (2) Based on morphological comparisons from HE-stained sections, the T2DM group exhibited the most significant alterations in the number of cells in the spiral ganglion, the organ of Corti, and the stria vascularis of the cochlea. (3) The expression level of the GRP78 protein in the cochlea was higher in the T2DM group compared to the OB group (P < 0.05) and higher in the OB group compared to the NC group (P < 0.05). Conclusion The findings indicate that the GRP78 protein plays a role in hearing loss caused by T2DM and hyperlipidemia. Moreover, T2DM is more likely than hyperlipidemia to be associated with hearing impairment.https://doi.org/10.1186/s13098-024-01255-6Auditory brainstem responseCochlea histologyDiabetic ratEndoplasmic reticulum stressObese rat |
| spellingShingle | Kai Xi Hua-Ping Li Yue-Hui Wang Yang-Yang Li Lei Wang Miao-Miao Zhang Xi Zhang Bing-Wen Xing GRP78 protein metabolism in obese and diabetic rats: a study of its role in metabolic disorders Diabetology & Metabolic Syndrome Auditory brainstem response Cochlea histology Diabetic rat Endoplasmic reticulum stress Obese rat |
| title | GRP78 protein metabolism in obese and diabetic rats: a study of its role in metabolic disorders |
| title_full | GRP78 protein metabolism in obese and diabetic rats: a study of its role in metabolic disorders |
| title_fullStr | GRP78 protein metabolism in obese and diabetic rats: a study of its role in metabolic disorders |
| title_full_unstemmed | GRP78 protein metabolism in obese and diabetic rats: a study of its role in metabolic disorders |
| title_short | GRP78 protein metabolism in obese and diabetic rats: a study of its role in metabolic disorders |
| title_sort | grp78 protein metabolism in obese and diabetic rats a study of its role in metabolic disorders |
| topic | Auditory brainstem response Cochlea histology Diabetic rat Endoplasmic reticulum stress Obese rat |
| url | https://doi.org/10.1186/s13098-024-01255-6 |
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