Utility of the Ratio between Lactate Dehydrogenase (LDH) Activity and Total Nucleated Cell Counts in Effusions (LDH/TNCC Ratio) for the Diagnosis of Feline Infectious Peritonitis (FIP)
Background: We tested the hypothesis that the ratio between lactate dehydrogenase activity (LDH) and total nucleated cell counts (TNCC) in effusions may be useful to diagnose feline infectious peritonitis (FIP). Methods: LDH/TNCC ratio was retrospectively evaluated in 648 effusions grouped based on...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-08-01
|
| Series: | Animals |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2615/12/17/2262 |
| _version_ | 1797496642497150976 |
|---|---|
| author | Pierpaolo Romanelli Saverio Paltrinieri Ugo Bonfanti Marco Graziano Castaman Elisa Monza Walter Bertazzolo |
| author_facet | Pierpaolo Romanelli Saverio Paltrinieri Ugo Bonfanti Marco Graziano Castaman Elisa Monza Walter Bertazzolo |
| author_sort | Pierpaolo Romanelli |
| collection | DOAJ |
| description | Background: We tested the hypothesis that the ratio between lactate dehydrogenase activity (LDH) and total nucleated cell counts (TNCC) in effusions may be useful to diagnose feline infectious peritonitis (FIP). Methods: LDH/TNCC ratio was retrospectively evaluated in 648 effusions grouped based on cytology and physicochemical analysis (step 1), on the probability of FIP estimated by additional tests on fluids (step 2) or on other biological samples (step 3, <i>n</i> = 471). Results of different steps were statistically compared. Receiver Operating Characteristic (ROC) curves were designed to assess whether the ratio identify the samples with FIP “probable/almost confirmed”. The cut-offs with the highest positive likelihood ratio (LR+) or Youden Index (YI) or with equal sensitivity and specificity were determined. Results: A high median LDH/TNCC ratio was found in FIP effusions (step1: 2.01) and with probable or almost confirmed FIP (step 2: 1.99; 2.20 respectively; step 3: 1.26; 2.30 respectively). The optimal cut-offs were 7.54 (LR+ 6.58), 0.62 (IY 0.67, sensitivity: 89.1%; specificity 77.7%), 0.72 (sensitivity and specificity: 79.2%) in step 2 and 2.27 (LR+ 10.39), 0.62 (IY 0.65, sensitivity: 82.1%; specificity 83.0%), 0.54 (sensitivity: 82.1%; specificity 81.9%) in step 3. Conclusions: a high LDH/TNCC ratio support a FIP diagnosis. |
| first_indexed | 2024-03-10T03:06:33Z |
| format | Article |
| id | doaj.art-60d81cf9dcf54c44ac9024cae23340c4 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T03:06:33Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-60d81cf9dcf54c44ac9024cae23340c42023-11-23T12:37:58ZengMDPI AGAnimals2076-26152022-08-011217226210.3390/ani12172262Utility of the Ratio between Lactate Dehydrogenase (LDH) Activity and Total Nucleated Cell Counts in Effusions (LDH/TNCC Ratio) for the Diagnosis of Feline Infectious Peritonitis (FIP)Pierpaolo Romanelli0Saverio Paltrinieri1Ugo Bonfanti2Marco Graziano Castaman3Elisa Monza4Walter Bertazzolo5MYLAV Veterinary Laboratory La Vallonea, 20017 Passirana di Rho, ItalyDepartment of Veterinary Medicine and Animal Sciences, University of Milan, 26900 Lodi, ItalyMYLAV Veterinary Laboratory La Vallonea, 20017 Passirana di Rho, ItalyMYLAV Veterinary Laboratory La Vallonea, 20017 Passirana di Rho, ItalyMYLAV Veterinary Laboratory La Vallonea, 20017 Passirana di Rho, ItalyMYLAV Veterinary Laboratory La Vallonea, 20017 Passirana di Rho, ItalyBackground: We tested the hypothesis that the ratio between lactate dehydrogenase activity (LDH) and total nucleated cell counts (TNCC) in effusions may be useful to diagnose feline infectious peritonitis (FIP). Methods: LDH/TNCC ratio was retrospectively evaluated in 648 effusions grouped based on cytology and physicochemical analysis (step 1), on the probability of FIP estimated by additional tests on fluids (step 2) or on other biological samples (step 3, <i>n</i> = 471). Results of different steps were statistically compared. Receiver Operating Characteristic (ROC) curves were designed to assess whether the ratio identify the samples with FIP “probable/almost confirmed”. The cut-offs with the highest positive likelihood ratio (LR+) or Youden Index (YI) or with equal sensitivity and specificity were determined. Results: A high median LDH/TNCC ratio was found in FIP effusions (step1: 2.01) and with probable or almost confirmed FIP (step 2: 1.99; 2.20 respectively; step 3: 1.26; 2.30 respectively). The optimal cut-offs were 7.54 (LR+ 6.58), 0.62 (IY 0.67, sensitivity: 89.1%; specificity 77.7%), 0.72 (sensitivity and specificity: 79.2%) in step 2 and 2.27 (LR+ 10.39), 0.62 (IY 0.65, sensitivity: 82.1%; specificity 83.0%), 0.54 (sensitivity: 82.1%; specificity 81.9%) in step 3. Conclusions: a high LDH/TNCC ratio support a FIP diagnosis.https://www.mdpi.com/2076-2615/12/17/2262catfeline coronavirus (FCoV)clinical chemistryeffusion cytologydiagnostic accuracy |
| spellingShingle | Pierpaolo Romanelli Saverio Paltrinieri Ugo Bonfanti Marco Graziano Castaman Elisa Monza Walter Bertazzolo Utility of the Ratio between Lactate Dehydrogenase (LDH) Activity and Total Nucleated Cell Counts in Effusions (LDH/TNCC Ratio) for the Diagnosis of Feline Infectious Peritonitis (FIP) Animals cat feline coronavirus (FCoV) clinical chemistry effusion cytology diagnostic accuracy |
| title | Utility of the Ratio between Lactate Dehydrogenase (LDH) Activity and Total Nucleated Cell Counts in Effusions (LDH/TNCC Ratio) for the Diagnosis of Feline Infectious Peritonitis (FIP) |
| title_full | Utility of the Ratio between Lactate Dehydrogenase (LDH) Activity and Total Nucleated Cell Counts in Effusions (LDH/TNCC Ratio) for the Diagnosis of Feline Infectious Peritonitis (FIP) |
| title_fullStr | Utility of the Ratio between Lactate Dehydrogenase (LDH) Activity and Total Nucleated Cell Counts in Effusions (LDH/TNCC Ratio) for the Diagnosis of Feline Infectious Peritonitis (FIP) |
| title_full_unstemmed | Utility of the Ratio between Lactate Dehydrogenase (LDH) Activity and Total Nucleated Cell Counts in Effusions (LDH/TNCC Ratio) for the Diagnosis of Feline Infectious Peritonitis (FIP) |
| title_short | Utility of the Ratio between Lactate Dehydrogenase (LDH) Activity and Total Nucleated Cell Counts in Effusions (LDH/TNCC Ratio) for the Diagnosis of Feline Infectious Peritonitis (FIP) |
| title_sort | utility of the ratio between lactate dehydrogenase ldh activity and total nucleated cell counts in effusions ldh tncc ratio for the diagnosis of feline infectious peritonitis fip |
| topic | cat feline coronavirus (FCoV) clinical chemistry effusion cytology diagnostic accuracy |
| url | https://www.mdpi.com/2076-2615/12/17/2262 |
| work_keys_str_mv | AT pierpaoloromanelli utilityoftheratiobetweenlactatedehydrogenaseldhactivityandtotalnucleatedcellcountsineffusionsldhtnccratioforthediagnosisoffelineinfectiousperitonitisfip AT saveriopaltrinieri utilityoftheratiobetweenlactatedehydrogenaseldhactivityandtotalnucleatedcellcountsineffusionsldhtnccratioforthediagnosisoffelineinfectiousperitonitisfip AT ugobonfanti utilityoftheratiobetweenlactatedehydrogenaseldhactivityandtotalnucleatedcellcountsineffusionsldhtnccratioforthediagnosisoffelineinfectiousperitonitisfip AT marcograzianocastaman utilityoftheratiobetweenlactatedehydrogenaseldhactivityandtotalnucleatedcellcountsineffusionsldhtnccratioforthediagnosisoffelineinfectiousperitonitisfip AT elisamonza utilityoftheratiobetweenlactatedehydrogenaseldhactivityandtotalnucleatedcellcountsineffusionsldhtnccratioforthediagnosisoffelineinfectiousperitonitisfip AT walterbertazzolo utilityoftheratiobetweenlactatedehydrogenaseldhactivityandtotalnucleatedcellcountsineffusionsldhtnccratioforthediagnosisoffelineinfectiousperitonitisfip |