Creating large EMS populations for functional genomics and breeding in wheat
Wheat germplasm is a fundamental resource for basic research, applied studies, and wheat breeding, which can be enriched normally by several paths, such as collecting natural lines, accumulating breeding lines, and introducing mutagenesis materials. Ethyl methane sulfonate (EMS) is an alkylating age...
| Main Authors: | , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2024-02-01
|
| Series: | Journal of Integrative Agriculture |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311923001648 |
| _version_ | 1797326296553881600 |
|---|---|
| author | Wenqiang Wang Xizhen Guan Yong Gan Guojun Liu Chunhao Zou Weikang Wang Jifa Zhang Huifei Zhang Qunqun Hao Fei Ni Jiajie Wu Lynn Epstein Daolin Fu |
| author_facet | Wenqiang Wang Xizhen Guan Yong Gan Guojun Liu Chunhao Zou Weikang Wang Jifa Zhang Huifei Zhang Qunqun Hao Fei Ni Jiajie Wu Lynn Epstein Daolin Fu |
| author_sort | Wenqiang Wang |
| collection | DOAJ |
| description | Wheat germplasm is a fundamental resource for basic research, applied studies, and wheat breeding, which can be enriched normally by several paths, such as collecting natural lines, accumulating breeding lines, and introducing mutagenesis materials. Ethyl methane sulfonate (EMS) is an alkylating agent that can effectively introduce genetic variations in a wide variety of plant species. In this study, we created a million-scale EMS population (MEP) that started with the Chinese wheat cultivars 'Luyan 128', 'Jimai 38', 'Jimai 44', and 'Shannong 30'. In the M1 generation, the MEP had numerous phenotypical variations, such as >3,000 chlorophyll-deficient mutants, 2,519 compact spikes, and 1,692 male sterile spikes. There were also rare mutations, including 30 independent tillers each with double heads. Some M1 variations of chlorophyll-deficiency and compact spikes were inheritable, appearing in the M2 or M3 generations. To advance the entire MEP to higher generations, we adopted a single-seed descendent (SSD) approach. All other seed composites of M2 were used to screen other agronomically important traits, such as the tolerance to herbicide quizalofop-P-methyl. The MEP is available for collaborative projects, and provides a valuable toolbox for wheat genetics and breeding for sustainable agriculture. |
| first_indexed | 2024-03-08T06:21:18Z |
| format | Article |
| id | doaj.art-60dd249195314f0e87d9a9be0b135c65 |
| institution | Directory Open Access Journal |
| issn | 2095-3119 |
| language | English |
| last_indexed | 2024-03-08T06:21:18Z |
| publishDate | 2024-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj.art-60dd249195314f0e87d9a9be0b135c652024-02-04T04:44:12ZengElsevierJournal of Integrative Agriculture2095-31192024-02-01232484493Creating large EMS populations for functional genomics and breeding in wheatWenqiang Wang0Xizhen Guan1Yong Gan2Guojun Liu3Chunhao Zou4Weikang Wang5Jifa Zhang6Huifei Zhang7Qunqun Hao8Fei Ni9Jiajie Wu10Lynn Epstein11Daolin Fu12Spring Valley Agriscience Co., Ltd., Jinan 250300, China; College of Life Sciences, Zaozhuang University, Zaozhuang 277160, ChinaSpring Valley Agriscience Co., Ltd., Jinan 250300, ChinaNational Key Laboratory of Wheat Improvement, College of Agronomy, Shandong Agricultural University, Tai'an 271018, ChinaNational Key Laboratory of Wheat Improvement, College of Agronomy, Shandong Agricultural University, Tai'an 271018, ChinaSpring Valley Agriscience Co., Ltd., Jinan 250300, ChinaSpring Valley Agriscience Co., Ltd., Jinan 250300, ChinaSpring Valley Agriscience Co., Ltd., Jinan 250300, ChinaSpring Valley Agriscience Co., Ltd., Jinan 250300, ChinaCollege of Life Sciences, Zaozhuang University, Zaozhuang 277160, ChinaNational Key Laboratory of Wheat Improvement, College of Agronomy, Shandong Agricultural University, Tai'an 271018, ChinaNational Key Laboratory of Wheat Improvement, College of Agronomy, Shandong Agricultural University, Tai'an 271018, ChinaDepartment of Plant Pathology, University of California, Davis, CA 95616, USASpring Valley Agriscience Co., Ltd., Jinan 250300, China; National Key Laboratory of Wheat Improvement, College of Agronomy, Shandong Agricultural University, Tai'an 271018, China; Correspondence Daolin Fu, Tel: +86-531-88766999Wheat germplasm is a fundamental resource for basic research, applied studies, and wheat breeding, which can be enriched normally by several paths, such as collecting natural lines, accumulating breeding lines, and introducing mutagenesis materials. Ethyl methane sulfonate (EMS) is an alkylating agent that can effectively introduce genetic variations in a wide variety of plant species. In this study, we created a million-scale EMS population (MEP) that started with the Chinese wheat cultivars 'Luyan 128', 'Jimai 38', 'Jimai 44', and 'Shannong 30'. In the M1 generation, the MEP had numerous phenotypical variations, such as >3,000 chlorophyll-deficient mutants, 2,519 compact spikes, and 1,692 male sterile spikes. There were also rare mutations, including 30 independent tillers each with double heads. Some M1 variations of chlorophyll-deficiency and compact spikes were inheritable, appearing in the M2 or M3 generations. To advance the entire MEP to higher generations, we adopted a single-seed descendent (SSD) approach. All other seed composites of M2 were used to screen other agronomically important traits, such as the tolerance to herbicide quizalofop-P-methyl. The MEP is available for collaborative projects, and provides a valuable toolbox for wheat genetics and breeding for sustainable agriculture.http://www.sciencedirect.com/science/article/pii/S2095311923001648wheatgermplasmethyl methane sulfonategenetics and breeding |
| spellingShingle | Wenqiang Wang Xizhen Guan Yong Gan Guojun Liu Chunhao Zou Weikang Wang Jifa Zhang Huifei Zhang Qunqun Hao Fei Ni Jiajie Wu Lynn Epstein Daolin Fu Creating large EMS populations for functional genomics and breeding in wheat Journal of Integrative Agriculture wheat germplasm ethyl methane sulfonate genetics and breeding |
| title | Creating large EMS populations for functional genomics and breeding in wheat |
| title_full | Creating large EMS populations for functional genomics and breeding in wheat |
| title_fullStr | Creating large EMS populations for functional genomics and breeding in wheat |
| title_full_unstemmed | Creating large EMS populations for functional genomics and breeding in wheat |
| title_short | Creating large EMS populations for functional genomics and breeding in wheat |
| title_sort | creating large ems populations for functional genomics and breeding in wheat |
| topic | wheat germplasm ethyl methane sulfonate genetics and breeding |
| url | http://www.sciencedirect.com/science/article/pii/S2095311923001648 |
| work_keys_str_mv | AT wenqiangwang creatinglargeemspopulationsforfunctionalgenomicsandbreedinginwheat AT xizhenguan creatinglargeemspopulationsforfunctionalgenomicsandbreedinginwheat AT yonggan creatinglargeemspopulationsforfunctionalgenomicsandbreedinginwheat AT guojunliu creatinglargeemspopulationsforfunctionalgenomicsandbreedinginwheat AT chunhaozou creatinglargeemspopulationsforfunctionalgenomicsandbreedinginwheat AT weikangwang creatinglargeemspopulationsforfunctionalgenomicsandbreedinginwheat AT jifazhang creatinglargeemspopulationsforfunctionalgenomicsandbreedinginwheat AT huifeizhang creatinglargeemspopulationsforfunctionalgenomicsandbreedinginwheat AT qunqunhao creatinglargeemspopulationsforfunctionalgenomicsandbreedinginwheat AT feini creatinglargeemspopulationsforfunctionalgenomicsandbreedinginwheat AT jiajiewu creatinglargeemspopulationsforfunctionalgenomicsandbreedinginwheat AT lynnepstein creatinglargeemspopulationsforfunctionalgenomicsandbreedinginwheat AT daolinfu creatinglargeemspopulationsforfunctionalgenomicsandbreedinginwheat |