Mixed dye degradation by Bacillus pseudomycoides and Acinetobacter haemolyticus isolated from industrial effluents: A combined affirmation with wetlab and in silico studies
The environment-friendly bacterial strains are used for wastewater treatment due to their high degrading capability and cost-effectiveness. In the present study, we isolated, identified, characterized, and optimized culture condition ( Temperature 35 °C for both, time up to 96 h, pH 7 and 7.5 respec...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2022-09-01
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| Series: | Arabian Journal of Chemistry |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S187853522200394X |
| _version_ | 1818518048705871872 |
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| author | Md. Shamim Hossain Gobindo Kumar Paul Shafi Mahmud Md. Abu Saleh Md. Salah Uddin Amit Kumar Dutta Apurba Kumar Roy Ananda Kumar Saha Md. Moinuddin Sheam Sabbir Ahmed Md. Mizanur Rahman Dipak Kumar Paul Sudhangshu Kumar Biswas |
| author_facet | Md. Shamim Hossain Gobindo Kumar Paul Shafi Mahmud Md. Abu Saleh Md. Salah Uddin Amit Kumar Dutta Apurba Kumar Roy Ananda Kumar Saha Md. Moinuddin Sheam Sabbir Ahmed Md. Mizanur Rahman Dipak Kumar Paul Sudhangshu Kumar Biswas |
| author_sort | Md. Shamim Hossain |
| collection | DOAJ |
| description | The environment-friendly bacterial strains are used for wastewater treatment due to their high degrading capability and cost-effectiveness. In the present study, we isolated, identified, characterized, and optimized culture condition ( Temperature 35 °C for both, time up to 96 h, pH 7 and 7.5 respectively) of two dye degrading bacteria from industrial effluents. Morphological, biochemical, and molecular identification confirmed those strains as Bacillus pseudomycoides and Acinetobacter haemolyticus. Spectrophotometric methods were used to investigate the dye degradation(single and mixed dye) capability of these two bacteria. These strains were the potential for degrading methylene green (MG), basic violet (BV) and acid blue (AB) dyes. In case of MG + BV, B. pseudomycoides, and A. haemolyticus showed a degradation rate of 74% and 75%, respectively. While degradation was found 75% and 82% for MG + AB combination, 73% and 73% for AB + BV combination, and 80% and 82% for MG + BV + AB combination respectively. Azoreductase enzymes from bacteria are essential for breaking down the azo bond in textile azo dyes. In molecular docking, the binding energy of three docking complexes (protein and MG, protein and BV, protein and AB) were −6.3, −6.6, and −6.8 Kcal/mol, respectively. The binding stability of the docked complexes was ensured by the root mean square deviations (RMSD), solvent accessible surface area (SASA), radius of gyration (Rg) and hydrogen bond in a molecular dynamics simulation study, indicating strong and stable binding. This study revealed that both B. pseudomycoides and A. haemolyticus could decolorize single and mixed dyes efficiently. As a result, both the strains could be used in further research to apply their potentiality in large-scale dye degradation in the future. |
| first_indexed | 2024-12-11T01:04:48Z |
| format | Article |
| id | doaj.art-60e1cab386964f3c9a1efc86635516cd |
| institution | Directory Open Access Journal |
| issn | 1878-5352 |
| language | English |
| last_indexed | 2024-12-11T01:04:48Z |
| publishDate | 2022-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Arabian Journal of Chemistry |
| spelling | doaj.art-60e1cab386964f3c9a1efc86635516cd2022-12-22T01:26:13ZengElsevierArabian Journal of Chemistry1878-53522022-09-01159104078Mixed dye degradation by Bacillus pseudomycoides and Acinetobacter haemolyticus isolated from industrial effluents: A combined affirmation with wetlab and in silico studiesMd. Shamim Hossain0Gobindo Kumar Paul1Shafi Mahmud2Md. Abu Saleh3Md. Salah Uddin4Amit Kumar Dutta5Apurba Kumar Roy6Ananda Kumar Saha7Md. Moinuddin Sheam8Sabbir Ahmed9Md. Mizanur Rahman10Dipak Kumar Paul11Sudhangshu Kumar Biswas12Department of Biotechnology and Genetic Engineering, Islamic University, Kushtia 7003, BangladeshMicrobiology Laboratory, Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi 6205, BangladeshMicrobiology Laboratory, Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi 6205, BangladeshMicrobiology Laboratory, Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi 6205, BangladeshMicrobiology Laboratory, Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi 6205, BangladeshDepartment of Microbiology, University of Rajshahi, Rajshahi 6205, BangladeshDepartment of Genetic Engineering and Biotechnology, Faculty of Biological Sciences, University of Rajshahi, Rajshahi 6205, BangladeshDepartment of Zoology, Faculty of Biological Sciences, University of Rajshahi, Rajshahi 6205, BangladeshDepartment of Biotechnology and Genetic Engineering, Islamic University, Kushtia 7003, BangladeshDepartment of Biotechnology and Genetic Engineering, Islamic University, Kushtia 7003, BangladeshDepartment of Biotechnology and Genetic Engineering, Islamic University, Kushtia 7003, BangladeshDepartment of Applied Nutrition and Food Technology, Islamic University, Kushtia 7003, BangladeshDepartment of Biotechnology and Genetic Engineering, Islamic University, Kushtia 7003, Bangladesh; Corresponding author.The environment-friendly bacterial strains are used for wastewater treatment due to their high degrading capability and cost-effectiveness. In the present study, we isolated, identified, characterized, and optimized culture condition ( Temperature 35 °C for both, time up to 96 h, pH 7 and 7.5 respectively) of two dye degrading bacteria from industrial effluents. Morphological, biochemical, and molecular identification confirmed those strains as Bacillus pseudomycoides and Acinetobacter haemolyticus. Spectrophotometric methods were used to investigate the dye degradation(single and mixed dye) capability of these two bacteria. These strains were the potential for degrading methylene green (MG), basic violet (BV) and acid blue (AB) dyes. In case of MG + BV, B. pseudomycoides, and A. haemolyticus showed a degradation rate of 74% and 75%, respectively. While degradation was found 75% and 82% for MG + AB combination, 73% and 73% for AB + BV combination, and 80% and 82% for MG + BV + AB combination respectively. Azoreductase enzymes from bacteria are essential for breaking down the azo bond in textile azo dyes. In molecular docking, the binding energy of three docking complexes (protein and MG, protein and BV, protein and AB) were −6.3, −6.6, and −6.8 Kcal/mol, respectively. The binding stability of the docked complexes was ensured by the root mean square deviations (RMSD), solvent accessible surface area (SASA), radius of gyration (Rg) and hydrogen bond in a molecular dynamics simulation study, indicating strong and stable binding. This study revealed that both B. pseudomycoides and A. haemolyticus could decolorize single and mixed dyes efficiently. As a result, both the strains could be used in further research to apply their potentiality in large-scale dye degradation in the future.http://www.sciencedirect.com/science/article/pii/S187853522200394XTextile effluentsMixed dye degradation16s rDNA sequencingMolecular docking |
| spellingShingle | Md. Shamim Hossain Gobindo Kumar Paul Shafi Mahmud Md. Abu Saleh Md. Salah Uddin Amit Kumar Dutta Apurba Kumar Roy Ananda Kumar Saha Md. Moinuddin Sheam Sabbir Ahmed Md. Mizanur Rahman Dipak Kumar Paul Sudhangshu Kumar Biswas Mixed dye degradation by Bacillus pseudomycoides and Acinetobacter haemolyticus isolated from industrial effluents: A combined affirmation with wetlab and in silico studies Arabian Journal of Chemistry Textile effluents Mixed dye degradation 16s rDNA sequencing Molecular docking |
| title | Mixed dye degradation by Bacillus pseudomycoides and Acinetobacter haemolyticus isolated from industrial effluents: A combined affirmation with wetlab and in silico studies |
| title_full | Mixed dye degradation by Bacillus pseudomycoides and Acinetobacter haemolyticus isolated from industrial effluents: A combined affirmation with wetlab and in silico studies |
| title_fullStr | Mixed dye degradation by Bacillus pseudomycoides and Acinetobacter haemolyticus isolated from industrial effluents: A combined affirmation with wetlab and in silico studies |
| title_full_unstemmed | Mixed dye degradation by Bacillus pseudomycoides and Acinetobacter haemolyticus isolated from industrial effluents: A combined affirmation with wetlab and in silico studies |
| title_short | Mixed dye degradation by Bacillus pseudomycoides and Acinetobacter haemolyticus isolated from industrial effluents: A combined affirmation with wetlab and in silico studies |
| title_sort | mixed dye degradation by bacillus pseudomycoides and acinetobacter haemolyticus isolated from industrial effluents a combined affirmation with wetlab and in silico studies |
| topic | Textile effluents Mixed dye degradation 16s rDNA sequencing Molecular docking |
| url | http://www.sciencedirect.com/science/article/pii/S187853522200394X |
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