Nationwide Screening for Bee Viruses and Parasites in Belgian Honey Bees

The health of honey bees is threatened by multiple factors, including viruses and parasites. We screened 557 honey bee (<i>Apis mellifera</i>) colonies from 155 beekeepers distributed all over Belgium to determine the prevalence of seven widespread viruses and two parasites (<i>Var...

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Bibliographic Details
Main Authors: Severine Matthijs, Valérie De Waele, Valerie Vandenberge, Bénédicte Verhoeven, Jacqueline Evers, Marleen Brunain, Claude Saegerman, Paul J. J. De Winter, Stefan Roels, Dirk C. de Graaf, Nick De Regge
Format: Article
Language:English
Published: MDPI AG 2020-08-01
Series:Viruses
Subjects:
Online Access:https://www.mdpi.com/1999-4915/12/8/890
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Summary:The health of honey bees is threatened by multiple factors, including viruses and parasites. We screened 557 honey bee (<i>Apis mellifera</i>) colonies from 155 beekeepers distributed all over Belgium to determine the prevalence of seven widespread viruses and two parasites (<i>Varroa</i> sp. and <i>Nosema</i> sp.). Deformed wing virus B (DWV-B), black queen cell virus (BQCV), and sacbrood virus (SBV) were highly prevalent and detected by real-time RT-PCR in more than 95% of the colonies. Acute bee paralysis virus (ABPV), chronic bee paralysis virus (CBPV) and deformed wing virus A (DWV-A) were prevalent to a lower extent (between 18 and 29%). Most viruses were only present at low or moderate viral loads. Nevertheless, about 50% of the colonies harbored at least one virus at high viral load (>10<sup>7</sup> genome copies/bee). <i>Varroa</i> mites and <i>Nosema</i> sp. were found in 81.5% and 59.7% of the honey bee colonies, respectively, and all <i>Nosema</i> were identified as <i>Nosema ceranae</i> by real time PCR. Interestingly, we found a significant correlation between the number of <i>Varroa</i> mites and DWV-B viral load. To determine the combined effect of these and other factors on honey bee health in Belgium, a follow up of colonies over multiple years is necessary.
ISSN:1999-4915