Impact of Mycobacteroides abscessus colony morphology on biofilm formation and antimicrobial resistance
Mycobacteroides abscessus is one of the most resistant bacteria so far known and causes severe and hard to treat lung infections in predisposed patients such as those with Cystic Fibrosis (CF). Further, it causes nosocomial infections by forming biofilms on medical devices or water reservoirs. An ey...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-03-01
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| Series: | International Journal of Medical Microbiology |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1438422124000079 |
| _version_ | 1797256413361209344 |
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| author | Anna Maria Oschmann-Kadenbach Christoph Schaudinn Leonard Borst Carsten Schwarz Katharina Konrat Mardjan Arvand Astrid Lewin |
| author_facet | Anna Maria Oschmann-Kadenbach Christoph Schaudinn Leonard Borst Carsten Schwarz Katharina Konrat Mardjan Arvand Astrid Lewin |
| author_sort | Anna Maria Oschmann-Kadenbach |
| collection | DOAJ |
| description | Mycobacteroides abscessus is one of the most resistant bacteria so far known and causes severe and hard to treat lung infections in predisposed patients such as those with Cystic Fibrosis (CF). Further, it causes nosocomial infections by forming biofilms on medical devices or water reservoirs. An eye-catching feature of M. abscessus is the growth in two colony morphotypes. Depending on the presence or absence of glycopeptidolipids on the cell surface, it forms smooth or rough colonies. In this study, a porous glass bead biofilm model was used to compare biofilm formation, biofilm organization and biofilm matrix composition in addition to the antimicrobial susceptibility of M. abscessus biofilms versus suspensions of isogenic (smooth and rough) patient isolates. Both morphotypes reached the same cell densities in biofilms. The biofilm architecture, however, was dramatically different with evenly distributed oligo-layered biofilms in smooth isolates, compared to tightly packed, voluminous biofilm clusters in rough morphotypes. Biofilms of both morphotypes contained more total biomass of the matrix components protein, lipid plus DNA than was seen in corresponding suspensions. The biofilm mode of growth of M. abscessus substantially increased resistance to the antibiotics amikacin and tigecycline. Tolerance to the disinfectant peracetic acid of both morphotypes was increased when grown as biofilm, while tolerance to glutaraldehyde was significantly increased in biofilm of smooth isolates only. Overall, smooth colony morphotypes had more pronounced antimicrobial resistance benefit when growing as biofilm than M. abscessus showing rough colony morphotypes. |
| first_indexed | 2024-03-08T12:47:19Z |
| format | Article |
| id | doaj.art-60e2f9614f72402eade5e57165ffb67f |
| institution | Directory Open Access Journal |
| issn | 1438-4221 |
| language | English |
| last_indexed | 2024-04-24T22:21:21Z |
| publishDate | 2024-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | International Journal of Medical Microbiology |
| spelling | doaj.art-60e2f9614f72402eade5e57165ffb67f2024-03-20T06:08:45ZengElsevierInternational Journal of Medical Microbiology1438-42212024-03-01314151603Impact of Mycobacteroides abscessus colony morphology on biofilm formation and antimicrobial resistanceAnna Maria Oschmann-Kadenbach0Christoph Schaudinn1Leonard Borst2Carsten Schwarz3Katharina Konrat4Mardjan Arvand5Astrid Lewin6Unit 14 Hospital Hygiene, Infection Prevention and Control, Robert Koch Institute, Seestr. 10, Berlin, Germany; Unit 16 Mycotic and Parasitic Agents and Mycobacteria, Robert Koch Institute, Seestr. 10, Berlin, GermanyUnit ZBS4 Advanced Light and Electron Microscopy, Robert Koch Institute, Seestr. 10, Berlin, GermanyUnit 16 Mycotic and Parasitic Agents and Mycobacteria, Robert Koch Institute, Seestr. 10, Berlin, GermanyCF Center Westbrandenburg, Division Cystic Fibrosis, Health and Medical University Potsdam and Clinic Westbrandenburg, Hebbelstraße 1, 14467 Potsdam, GermanyUnit 14 Hospital Hygiene, Infection Prevention and Control, Robert Koch Institute, Seestr. 10, Berlin, GermanyUnit 14 Hospital Hygiene, Infection Prevention and Control, Robert Koch Institute, Seestr. 10, Berlin, GermanyUnit 16 Mycotic and Parasitic Agents and Mycobacteria, Robert Koch Institute, Seestr. 10, Berlin, Germany; Correspondence to: Unit 16 Mycotic and Parasitic Agents and Mycobacteria, Robert Koch Institute, Seestr. 10, 13353 Berlin, Germany.Mycobacteroides abscessus is one of the most resistant bacteria so far known and causes severe and hard to treat lung infections in predisposed patients such as those with Cystic Fibrosis (CF). Further, it causes nosocomial infections by forming biofilms on medical devices or water reservoirs. An eye-catching feature of M. abscessus is the growth in two colony morphotypes. Depending on the presence or absence of glycopeptidolipids on the cell surface, it forms smooth or rough colonies. In this study, a porous glass bead biofilm model was used to compare biofilm formation, biofilm organization and biofilm matrix composition in addition to the antimicrobial susceptibility of M. abscessus biofilms versus suspensions of isogenic (smooth and rough) patient isolates. Both morphotypes reached the same cell densities in biofilms. The biofilm architecture, however, was dramatically different with evenly distributed oligo-layered biofilms in smooth isolates, compared to tightly packed, voluminous biofilm clusters in rough morphotypes. Biofilms of both morphotypes contained more total biomass of the matrix components protein, lipid plus DNA than was seen in corresponding suspensions. The biofilm mode of growth of M. abscessus substantially increased resistance to the antibiotics amikacin and tigecycline. Tolerance to the disinfectant peracetic acid of both morphotypes was increased when grown as biofilm, while tolerance to glutaraldehyde was significantly increased in biofilm of smooth isolates only. Overall, smooth colony morphotypes had more pronounced antimicrobial resistance benefit when growing as biofilm than M. abscessus showing rough colony morphotypes.http://www.sciencedirect.com/science/article/pii/S1438422124000079Nontuberculous mycobacteriaMycobacteroides abscessusBiofilmAntibiotic resistanceDisinfectant toleranceColony morphotype |
| spellingShingle | Anna Maria Oschmann-Kadenbach Christoph Schaudinn Leonard Borst Carsten Schwarz Katharina Konrat Mardjan Arvand Astrid Lewin Impact of Mycobacteroides abscessus colony morphology on biofilm formation and antimicrobial resistance International Journal of Medical Microbiology Nontuberculous mycobacteria Mycobacteroides abscessus Biofilm Antibiotic resistance Disinfectant tolerance Colony morphotype |
| title | Impact of Mycobacteroides abscessus colony morphology on biofilm formation and antimicrobial resistance |
| title_full | Impact of Mycobacteroides abscessus colony morphology on biofilm formation and antimicrobial resistance |
| title_fullStr | Impact of Mycobacteroides abscessus colony morphology on biofilm formation and antimicrobial resistance |
| title_full_unstemmed | Impact of Mycobacteroides abscessus colony morphology on biofilm formation and antimicrobial resistance |
| title_short | Impact of Mycobacteroides abscessus colony morphology on biofilm formation and antimicrobial resistance |
| title_sort | impact of mycobacteroides abscessus colony morphology on biofilm formation and antimicrobial resistance |
| topic | Nontuberculous mycobacteria Mycobacteroides abscessus Biofilm Antibiotic resistance Disinfectant tolerance Colony morphotype |
| url | http://www.sciencedirect.com/science/article/pii/S1438422124000079 |
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