OCT Meets micro-CT: A Subject-Specific Correlative Multimodal Imaging Workflow for Early Chick Heart Development Modeling
Structural and Doppler velocity data collected from optical coherence tomography have already provided crucial insights into cardiac morphogenesis. X-ray microtomography and other ex vivo methods have elucidated structural details of developing hearts. However, by itself, no single imaging modality...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-11-01
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| Series: | Journal of Cardiovascular Development and Disease |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2308-3425/9/11/379 |
| _version_ | 1827646425597476864 |
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| author | Nina Kraus Fabian Placzek Brian Metscher |
| author_facet | Nina Kraus Fabian Placzek Brian Metscher |
| author_sort | Nina Kraus |
| collection | DOAJ |
| description | Structural and Doppler velocity data collected from optical coherence tomography have already provided crucial insights into cardiac morphogenesis. X-ray microtomography and other ex vivo methods have elucidated structural details of developing hearts. However, by itself, no single imaging modality can provide comprehensive information allowing to fully decipher the inner workings of an entire developing organ. Hence, we introduce a specimen-specific correlative multimodal imaging workflow combining OCT and micro-CT imaging which is applicable for modeling of early chick heart development—a valuable model organism in cardiovascular development research. The image acquisition and processing employ common reagents, lab-based micro-CT imaging, and software that is free for academic use. Our goal is to provide a step-by-step guide on how to implement this workflow and to demonstrate why those two modalities together have the potential to provide new insight into normal cardiac development and heart malformations leading to congenital heart disease. |
| first_indexed | 2024-03-09T18:58:37Z |
| format | Article |
| id | doaj.art-60e3dac80e654aa0a5c5ae7abe7da22e |
| institution | Directory Open Access Journal |
| issn | 2308-3425 |
| language | English |
| last_indexed | 2024-03-09T18:58:37Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Cardiovascular Development and Disease |
| spelling | doaj.art-60e3dac80e654aa0a5c5ae7abe7da22e2023-11-24T05:14:10ZengMDPI AGJournal of Cardiovascular Development and Disease2308-34252022-11-0191137910.3390/jcdd9110379OCT Meets micro-CT: A Subject-Specific Correlative Multimodal Imaging Workflow for Early Chick Heart Development ModelingNina Kraus0Fabian Placzek1Brian Metscher2Department of Evolutionary Biology, University of Vienna, 1030 Vienna, AustriaCenter for Medical Physics and Biomedical Engineering, Medical University of Vienna, 1090 Vienna, AustriaDepartment of Evolutionary Biology, University of Vienna, 1030 Vienna, AustriaStructural and Doppler velocity data collected from optical coherence tomography have already provided crucial insights into cardiac morphogenesis. X-ray microtomography and other ex vivo methods have elucidated structural details of developing hearts. However, by itself, no single imaging modality can provide comprehensive information allowing to fully decipher the inner workings of an entire developing organ. Hence, we introduce a specimen-specific correlative multimodal imaging workflow combining OCT and micro-CT imaging which is applicable for modeling of early chick heart development—a valuable model organism in cardiovascular development research. The image acquisition and processing employ common reagents, lab-based micro-CT imaging, and software that is free for academic use. Our goal is to provide a step-by-step guide on how to implement this workflow and to demonstrate why those two modalities together have the potential to provide new insight into normal cardiac development and heart malformations leading to congenital heart disease.https://www.mdpi.com/2308-3425/9/11/379heart developmentoptical coherence tomographymicro-CTcorrelative imagingmultimodal imagingchick embryo |
| spellingShingle | Nina Kraus Fabian Placzek Brian Metscher OCT Meets micro-CT: A Subject-Specific Correlative Multimodal Imaging Workflow for Early Chick Heart Development Modeling Journal of Cardiovascular Development and Disease heart development optical coherence tomography micro-CT correlative imaging multimodal imaging chick embryo |
| title | OCT Meets micro-CT: A Subject-Specific Correlative Multimodal Imaging Workflow for Early Chick Heart Development Modeling |
| title_full | OCT Meets micro-CT: A Subject-Specific Correlative Multimodal Imaging Workflow for Early Chick Heart Development Modeling |
| title_fullStr | OCT Meets micro-CT: A Subject-Specific Correlative Multimodal Imaging Workflow for Early Chick Heart Development Modeling |
| title_full_unstemmed | OCT Meets micro-CT: A Subject-Specific Correlative Multimodal Imaging Workflow for Early Chick Heart Development Modeling |
| title_short | OCT Meets micro-CT: A Subject-Specific Correlative Multimodal Imaging Workflow for Early Chick Heart Development Modeling |
| title_sort | oct meets micro ct a subject specific correlative multimodal imaging workflow for early chick heart development modeling |
| topic | heart development optical coherence tomography micro-CT correlative imaging multimodal imaging chick embryo |
| url | https://www.mdpi.com/2308-3425/9/11/379 |
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