Serine carboxypeptidase 46 Regulates Grain Filling and Seed Germination in Rice (Oryza sativa L.).
Serine carboxypeptidase (SCP) is one of the largest groups of enzymes catalyzing proteolysis for functional protein maturation. To date, little is known about the function of SCPs in rice. In this study, we present a comprehensive analysis of the gene structure and expression profile of 59 rice SCPs...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2016-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4957776?pdf=render |
| _version_ | 1818066916259921920 |
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| author | Zhiyong Li Liqun Tang Jiehua Qiu Wen Zhang Yifeng Wang Xiaohong Tong Xiangjin Wei Yuxuan Hou Jian Zhang |
| author_facet | Zhiyong Li Liqun Tang Jiehua Qiu Wen Zhang Yifeng Wang Xiaohong Tong Xiangjin Wei Yuxuan Hou Jian Zhang |
| author_sort | Zhiyong Li |
| collection | DOAJ |
| description | Serine carboxypeptidase (SCP) is one of the largest groups of enzymes catalyzing proteolysis for functional protein maturation. To date, little is known about the function of SCPs in rice. In this study, we present a comprehensive analysis of the gene structure and expression profile of 59 rice SCPs. SCP46 is dominantly expressed in developing seeds, particularly in embryo, endosperm and aleurone layers, and could be induced by ABA. Functional characterization revealed that knock-down of SCP46 resulted in smaller grain size and enhanced seed germination. Furthermore, scp46 seed germination became less sensitive to the ABA inhibition than the Wild-type did; suggesting SCP46 is involved in ABA signaling. As indicated by RNA-seq and qRT-PCR analysis, numerous grain filling and seed dormancy related genes, such as SP, VP1 and AGPs were down-regulated in scp46. Yeast-two-hybrid assay also showed that SCP46 interacts with another ABA-inducible protein DI19-1. Taken together, we suggested that SCP46 is a master regulator of grain filling and seed germination, possibly via participating in the ABA signaling. The results of this study shed novel light into the roles of SCPs in rice. |
| first_indexed | 2024-12-10T15:15:23Z |
| format | Article |
| id | doaj.art-60e4cba7eee6425f8e0c89fbc215a689 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-10T15:15:23Z |
| publishDate | 2016-01-01 |
| publisher | Public Library of Science (PLoS) |
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| series | PLoS ONE |
| spelling | doaj.art-60e4cba7eee6425f8e0c89fbc215a6892022-12-22T01:43:48ZengPublic Library of Science (PLoS)PLoS ONE1932-62032016-01-01117e015973710.1371/journal.pone.0159737Serine carboxypeptidase 46 Regulates Grain Filling and Seed Germination in Rice (Oryza sativa L.).Zhiyong LiLiqun TangJiehua QiuWen ZhangYifeng WangXiaohong TongXiangjin WeiYuxuan HouJian ZhangSerine carboxypeptidase (SCP) is one of the largest groups of enzymes catalyzing proteolysis for functional protein maturation. To date, little is known about the function of SCPs in rice. In this study, we present a comprehensive analysis of the gene structure and expression profile of 59 rice SCPs. SCP46 is dominantly expressed in developing seeds, particularly in embryo, endosperm and aleurone layers, and could be induced by ABA. Functional characterization revealed that knock-down of SCP46 resulted in smaller grain size and enhanced seed germination. Furthermore, scp46 seed germination became less sensitive to the ABA inhibition than the Wild-type did; suggesting SCP46 is involved in ABA signaling. As indicated by RNA-seq and qRT-PCR analysis, numerous grain filling and seed dormancy related genes, such as SP, VP1 and AGPs were down-regulated in scp46. Yeast-two-hybrid assay also showed that SCP46 interacts with another ABA-inducible protein DI19-1. Taken together, we suggested that SCP46 is a master regulator of grain filling and seed germination, possibly via participating in the ABA signaling. The results of this study shed novel light into the roles of SCPs in rice.http://europepmc.org/articles/PMC4957776?pdf=render |
| spellingShingle | Zhiyong Li Liqun Tang Jiehua Qiu Wen Zhang Yifeng Wang Xiaohong Tong Xiangjin Wei Yuxuan Hou Jian Zhang Serine carboxypeptidase 46 Regulates Grain Filling and Seed Germination in Rice (Oryza sativa L.). PLoS ONE |
| title | Serine carboxypeptidase 46 Regulates Grain Filling and Seed Germination in Rice (Oryza sativa L.). |
| title_full | Serine carboxypeptidase 46 Regulates Grain Filling and Seed Germination in Rice (Oryza sativa L.). |
| title_fullStr | Serine carboxypeptidase 46 Regulates Grain Filling and Seed Germination in Rice (Oryza sativa L.). |
| title_full_unstemmed | Serine carboxypeptidase 46 Regulates Grain Filling and Seed Germination in Rice (Oryza sativa L.). |
| title_short | Serine carboxypeptidase 46 Regulates Grain Filling and Seed Germination in Rice (Oryza sativa L.). |
| title_sort | serine carboxypeptidase 46 regulates grain filling and seed germination in rice oryza sativa l |
| url | http://europepmc.org/articles/PMC4957776?pdf=render |
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