Biodeteriogens Characterization and Molecular Analyses of Diverse Funeral Accessories from XVII Century
A historical crypt offers us a particular view of the conditions of some buried materials (in this case textiles) and the various biogenic phenomena to which they were subjected over the centuries. In addition, significant knowledge can come by studying the DNA of buried objects which allows the rec...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-08-01
|
| Series: | Applied Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-3417/10/16/5451 |
| _version_ | 1797559902763220992 |
|---|---|
| author | Zuzana Kisová Matej Planý Jelena Pavlović Mária Bučková Andrea Puškárová Lucia Kraková Magdaléna Kapustová Domenico Pangallo Katarína Šoltys |
| author_facet | Zuzana Kisová Matej Planý Jelena Pavlović Mária Bučková Andrea Puškárová Lucia Kraková Magdaléna Kapustová Domenico Pangallo Katarína Šoltys |
| author_sort | Zuzana Kisová |
| collection | DOAJ |
| description | A historical crypt offers us a particular view of the conditions of some buried materials (in this case textiles) and the various biogenic phenomena to which they were subjected over the centuries. In addition, significant knowledge can come by studying the DNA of buried objects which allows the recognition of materials, but also to reveal some practice of the funeral ceremony. In this study, the deteriorating microbial communities colonizing various funeral textile items were identified and characterized using microscopic observation, cultivation, polymerase chain reaction (PCR) and sequencing, hydrolytic tests; and culture-independent analysis (high-throughput sequencing, MinION platform). Different PCR assays and consequent sequencing of amplicons were employed to recognize the animal origin of bodice reinforcements and the type of plant used to embellish the young girl. The analysis of ancient DNA (aDNA from animal and plant) was also completed by the application of high-throughput sequencing through Illumina platform. The combination of all these techniques permitted the identification of a complex microbiota composed by dangerous degradative microorganisms able to hydrolyze various organic substrates such as fibroin, keratin, and cellulose. Bacteria responsible for metal corrosion and bio-mineralization, and entomopathogenic and phytopathogenic fungi. The analysis of aDNA identified the animal component used in bodice manufacturing, the plant utilized as ornament and probably the season of this fatal event. |
| first_indexed | 2024-03-10T17:51:47Z |
| format | Article |
| id | doaj.art-60e4de6bc00f49a0b40114c9c51d4619 |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-10T17:51:47Z |
| publishDate | 2020-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-60e4de6bc00f49a0b40114c9c51d46192023-11-20T09:20:13ZengMDPI AGApplied Sciences2076-34172020-08-011016545110.3390/app10165451Biodeteriogens Characterization and Molecular Analyses of Diverse Funeral Accessories from XVII CenturyZuzana Kisová0Matej Planý1Jelena Pavlović2Mária Bučková3Andrea Puškárová4Lucia Kraková5Magdaléna Kapustová6Domenico Pangallo7Katarína Šoltys8Institute of Molecular Biology, Slovak Academy of Sciences, Dúbravská Cesta 21, 84551 Bratislava, SlovakiaInstitute of Molecular Biology, Slovak Academy of Sciences, Dúbravská Cesta 21, 84551 Bratislava, SlovakiaInstitute of Molecular Biology, Slovak Academy of Sciences, Dúbravská Cesta 21, 84551 Bratislava, SlovakiaInstitute of Molecular Biology, Slovak Academy of Sciences, Dúbravská Cesta 21, 84551 Bratislava, SlovakiaInstitute of Molecular Biology, Slovak Academy of Sciences, Dúbravská Cesta 21, 84551 Bratislava, SlovakiaInstitute of Molecular Biology, Slovak Academy of Sciences, Dúbravská Cesta 21, 84551 Bratislava, SlovakiaInstitute of Molecular Biology, Slovak Academy of Sciences, Dúbravská Cesta 21, 84551 Bratislava, SlovakiaInstitute of Molecular Biology, Slovak Academy of Sciences, Dúbravská Cesta 21, 84551 Bratislava, SlovakiaDepartment of Microbiology and Virology, Faculty of Natural Sciences, Comenius University in Bratislava, Ilkovičova 3278/6, 841 04 Bratislava, SlovakiaA historical crypt offers us a particular view of the conditions of some buried materials (in this case textiles) and the various biogenic phenomena to which they were subjected over the centuries. In addition, significant knowledge can come by studying the DNA of buried objects which allows the recognition of materials, but also to reveal some practice of the funeral ceremony. In this study, the deteriorating microbial communities colonizing various funeral textile items were identified and characterized using microscopic observation, cultivation, polymerase chain reaction (PCR) and sequencing, hydrolytic tests; and culture-independent analysis (high-throughput sequencing, MinION platform). Different PCR assays and consequent sequencing of amplicons were employed to recognize the animal origin of bodice reinforcements and the type of plant used to embellish the young girl. The analysis of ancient DNA (aDNA from animal and plant) was also completed by the application of high-throughput sequencing through Illumina platform. The combination of all these techniques permitted the identification of a complex microbiota composed by dangerous degradative microorganisms able to hydrolyze various organic substrates such as fibroin, keratin, and cellulose. Bacteria responsible for metal corrosion and bio-mineralization, and entomopathogenic and phytopathogenic fungi. The analysis of aDNA identified the animal component used in bodice manufacturing, the plant utilized as ornament and probably the season of this fatal event.https://www.mdpi.com/2076-3417/10/16/5451microbial communitybiodeteriorationhigh-throughput sequencingMinION approachSEMaDNA |
| spellingShingle | Zuzana Kisová Matej Planý Jelena Pavlović Mária Bučková Andrea Puškárová Lucia Kraková Magdaléna Kapustová Domenico Pangallo Katarína Šoltys Biodeteriogens Characterization and Molecular Analyses of Diverse Funeral Accessories from XVII Century Applied Sciences microbial community biodeterioration high-throughput sequencing MinION approach SEM aDNA |
| title | Biodeteriogens Characterization and Molecular Analyses of Diverse Funeral Accessories from XVII Century |
| title_full | Biodeteriogens Characterization and Molecular Analyses of Diverse Funeral Accessories from XVII Century |
| title_fullStr | Biodeteriogens Characterization and Molecular Analyses of Diverse Funeral Accessories from XVII Century |
| title_full_unstemmed | Biodeteriogens Characterization and Molecular Analyses of Diverse Funeral Accessories from XVII Century |
| title_short | Biodeteriogens Characterization and Molecular Analyses of Diverse Funeral Accessories from XVII Century |
| title_sort | biodeteriogens characterization and molecular analyses of diverse funeral accessories from xvii century |
| topic | microbial community biodeterioration high-throughput sequencing MinION approach SEM aDNA |
| url | https://www.mdpi.com/2076-3417/10/16/5451 |
| work_keys_str_mv | AT zuzanakisova biodeteriogenscharacterizationandmolecularanalysesofdiversefuneralaccessoriesfromxviicentury AT matejplany biodeteriogenscharacterizationandmolecularanalysesofdiversefuneralaccessoriesfromxviicentury AT jelenapavlovic biodeteriogenscharacterizationandmolecularanalysesofdiversefuneralaccessoriesfromxviicentury AT mariabuckova biodeteriogenscharacterizationandmolecularanalysesofdiversefuneralaccessoriesfromxviicentury AT andreapuskarova biodeteriogenscharacterizationandmolecularanalysesofdiversefuneralaccessoriesfromxviicentury AT luciakrakova biodeteriogenscharacterizationandmolecularanalysesofdiversefuneralaccessoriesfromxviicentury AT magdalenakapustova biodeteriogenscharacterizationandmolecularanalysesofdiversefuneralaccessoriesfromxviicentury AT domenicopangallo biodeteriogenscharacterizationandmolecularanalysesofdiversefuneralaccessoriesfromxviicentury AT katarinasoltys biodeteriogenscharacterizationandmolecularanalysesofdiversefuneralaccessoriesfromxviicentury |