Addressing safety concerns of long-term probiotic use: In vivo evidence from a rat model
The global market for probiotic supplements is continually expanding. However, the certainty of their safety is still a matter of concern. Since multi-strain probiotics are gaining increasing interest in clinical practice, the aim of the presented study was to evaluate the long-term effects of probi...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-05-01
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| Series: | Journal of Functional Foods |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464623001214 |
| _version_ | 1797837095307313152 |
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| author | Petra Hradicka Petra Adamkova Ludovit Lenhardt Sona Gancarcikova Silvia Farkasova Iannaccone Vlasta Demeckova |
| author_facet | Petra Hradicka Petra Adamkova Ludovit Lenhardt Sona Gancarcikova Silvia Farkasova Iannaccone Vlasta Demeckova |
| author_sort | Petra Hradicka |
| collection | DOAJ |
| description | The global market for probiotic supplements is continually expanding. However, the certainty of their safety is still a matter of concern. Since multi-strain probiotics are gaining increasing interest in clinical practice, the aim of the presented study was to evaluate the long-term effects of probiotic mixture on healthy Wistar rats with the focus on colon histology, haematology, serum biochemistry, inflammatory cytokine production and faecal microbiota composition. The long-term supplementation resulted in systemic pro-inflammatory response accompanied by increased abundance of bacterial families associated with the promotion of gastrointestinal inflammation. The important indicators of cardiovascular risk were significantly elevated after long-term probiotic treatment. Even though no histopathological lesions were detected in the colon, enlarged lymphoid aggregates and follicles were observed in probiotic-fed rats. Based on our study, commercially available probiotics for human use which are recommended as prophylaxis should be monitored for possible side effect after long-term usage. Our study thus contributes to the increasingly debated proposal of the scientific community which suggests the need to shift the future of probiotic prescription from the current ‘one-size-fits-all’ scheme into a person/condition-tailored approach with defined time of administration. |
| first_indexed | 2024-04-09T15:20:22Z |
| format | Article |
| id | doaj.art-60e834d9448d4bc19c9d41f02e8ab45a |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-04-09T15:20:22Z |
| publishDate | 2023-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-60e834d9448d4bc19c9d41f02e8ab45a2023-04-29T14:48:25ZengElsevierJournal of Functional Foods1756-46462023-05-01104105521Addressing safety concerns of long-term probiotic use: In vivo evidence from a rat modelPetra Hradicka0Petra Adamkova1Ludovit Lenhardt2Sona Gancarcikova3Silvia Farkasova Iannaccone4Vlasta Demeckova5Institute of Biology and Ecology, Faculty of Science, Pavol Jozef Safarik University in Kosice, Srobarova 2, Kosice 041 54, Slovak RepublicInstitute of Biology and Ecology, Faculty of Science, Pavol Jozef Safarik University in Kosice, Srobarova 2, Kosice 041 54, Slovak RepublicDepartment of Pathological Anatomy, University of Veterinary Medicine and Pharmacy, Komenskeho 73, 041 81 Kosice, Slovak RepublicDepartment of Microbiology and Immunology, University of Veterinary Medicine and Pharmacy, Komenskeho 73, 041 81 Kosice, Slovak RepublicDepartment of Forensic Medicine, Faculty of Medicine, Pavol Jozef Safarik University in Kosice, Trieda SNP 1, 04011 Kosice, Slovak RepublicInstitute of Biology and Ecology, Faculty of Science, Pavol Jozef Safarik University in Kosice, Srobarova 2, Kosice 041 54, Slovak Republic; Corresponding author.The global market for probiotic supplements is continually expanding. However, the certainty of their safety is still a matter of concern. Since multi-strain probiotics are gaining increasing interest in clinical practice, the aim of the presented study was to evaluate the long-term effects of probiotic mixture on healthy Wistar rats with the focus on colon histology, haematology, serum biochemistry, inflammatory cytokine production and faecal microbiota composition. The long-term supplementation resulted in systemic pro-inflammatory response accompanied by increased abundance of bacterial families associated with the promotion of gastrointestinal inflammation. The important indicators of cardiovascular risk were significantly elevated after long-term probiotic treatment. Even though no histopathological lesions were detected in the colon, enlarged lymphoid aggregates and follicles were observed in probiotic-fed rats. Based on our study, commercially available probiotics for human use which are recommended as prophylaxis should be monitored for possible side effect after long-term usage. Our study thus contributes to the increasingly debated proposal of the scientific community which suggests the need to shift the future of probiotic prescription from the current ‘one-size-fits-all’ scheme into a person/condition-tailored approach with defined time of administration.http://www.sciencedirect.com/science/article/pii/S1756464623001214Lactobacilli mixtureCytokineFaecal microbiota |
| spellingShingle | Petra Hradicka Petra Adamkova Ludovit Lenhardt Sona Gancarcikova Silvia Farkasova Iannaccone Vlasta Demeckova Addressing safety concerns of long-term probiotic use: In vivo evidence from a rat model Journal of Functional Foods Lactobacilli mixture Cytokine Faecal microbiota |
| title | Addressing safety concerns of long-term probiotic use: In vivo evidence from a rat model |
| title_full | Addressing safety concerns of long-term probiotic use: In vivo evidence from a rat model |
| title_fullStr | Addressing safety concerns of long-term probiotic use: In vivo evidence from a rat model |
| title_full_unstemmed | Addressing safety concerns of long-term probiotic use: In vivo evidence from a rat model |
| title_short | Addressing safety concerns of long-term probiotic use: In vivo evidence from a rat model |
| title_sort | addressing safety concerns of long term probiotic use in vivo evidence from a rat model |
| topic | Lactobacilli mixture Cytokine Faecal microbiota |
| url | http://www.sciencedirect.com/science/article/pii/S1756464623001214 |
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