Quantitative Threshold Determination of Auditory Brainstem Responses in Mouse Models
The auditory brainstem response (ABR) is a scalp recording of potentials produced by sound stimulation, and is commonly used as an indicator of auditory function. However, the ABR threshold, which is the lowest audible sound pressure, cannot be objectively determined since it is determined visually...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-07-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/14/11393 |
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| author | Kenji Tanaka Shuma Ohara Tadaaki Matsuzaka Aira Matsugaki Takuya Ishimoto Ryosuke Ozasa Yukiko Kuroda Koichi Matsuo Takayoshi Nakano |
| author_facet | Kenji Tanaka Shuma Ohara Tadaaki Matsuzaka Aira Matsugaki Takuya Ishimoto Ryosuke Ozasa Yukiko Kuroda Koichi Matsuo Takayoshi Nakano |
| author_sort | Kenji Tanaka |
| collection | DOAJ |
| description | The auditory brainstem response (ABR) is a scalp recording of potentials produced by sound stimulation, and is commonly used as an indicator of auditory function. However, the ABR threshold, which is the lowest audible sound pressure, cannot be objectively determined since it is determined visually using a measurer, and this has been a problem for several decades. Although various algorithms have been developed to objectively determine ABR thresholds, they remain lacking in terms of accuracy, efficiency, and convenience. Accordingly, we proposed an improved algorithm based on the mutual covariance at adjacent sound pressure levels. An ideal ABR waveform with clearly defined waves I–V was created; moreover, using this waveform as a standard template, the experimentally obtained ABR waveform was inspected for disturbances based on mutual covariance. The ABR testing was repeated if the value was below the established cross-covariance reference value. Our proposed method allowed more efficient objective determination of ABR thresholds and a smaller burden on experimental animals. |
| first_indexed | 2024-03-11T01:00:38Z |
| format | Article |
| id | doaj.art-60ecd0d7d2f64be0a9f89d6a7a1701ca |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T01:00:38Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-60ecd0d7d2f64be0a9f89d6a7a1701ca2023-11-18T19:38:43ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-07-0124141139310.3390/ijms241411393Quantitative Threshold Determination of Auditory Brainstem Responses in Mouse ModelsKenji Tanaka0Shuma Ohara1Tadaaki Matsuzaka2Aira Matsugaki3Takuya Ishimoto4Ryosuke Ozasa5Yukiko Kuroda6Koichi Matsuo7Takayoshi Nakano8Division of Materials and Manufacturing Science, Graduate School of Engineering, Osaka University, Suita 565-0871, JapanDivision of Materials and Manufacturing Science, Graduate School of Engineering, Osaka University, Suita 565-0871, JapanDivision of Materials and Manufacturing Science, Graduate School of Engineering, Osaka University, Suita 565-0871, JapanDivision of Materials and Manufacturing Science, Graduate School of Engineering, Osaka University, Suita 565-0871, JapanDivision of Materials and Manufacturing Science, Graduate School of Engineering, Osaka University, Suita 565-0871, JapanDivision of Materials and Manufacturing Science, Graduate School of Engineering, Osaka University, Suita 565-0871, JapanLaboratory of Cell and Tissue Biology, Keio University School of Medicine, Tokyo 160-8582, JapanLaboratory of Cell and Tissue Biology, Keio University School of Medicine, Tokyo 160-8582, JapanDivision of Materials and Manufacturing Science, Graduate School of Engineering, Osaka University, Suita 565-0871, JapanThe auditory brainstem response (ABR) is a scalp recording of potentials produced by sound stimulation, and is commonly used as an indicator of auditory function. However, the ABR threshold, which is the lowest audible sound pressure, cannot be objectively determined since it is determined visually using a measurer, and this has been a problem for several decades. Although various algorithms have been developed to objectively determine ABR thresholds, they remain lacking in terms of accuracy, efficiency, and convenience. Accordingly, we proposed an improved algorithm based on the mutual covariance at adjacent sound pressure levels. An ideal ABR waveform with clearly defined waves I–V was created; moreover, using this waveform as a standard template, the experimentally obtained ABR waveform was inspected for disturbances based on mutual covariance. The ABR testing was repeated if the value was below the established cross-covariance reference value. Our proposed method allowed more efficient objective determination of ABR thresholds and a smaller burden on experimental animals.https://www.mdpi.com/1422-0067/24/14/11393auditory brainstem responses (ABR)auditory functionABR thresholdcorrelation coefficientcross-covariance |
| spellingShingle | Kenji Tanaka Shuma Ohara Tadaaki Matsuzaka Aira Matsugaki Takuya Ishimoto Ryosuke Ozasa Yukiko Kuroda Koichi Matsuo Takayoshi Nakano Quantitative Threshold Determination of Auditory Brainstem Responses in Mouse Models International Journal of Molecular Sciences auditory brainstem responses (ABR) auditory function ABR threshold correlation coefficient cross-covariance |
| title | Quantitative Threshold Determination of Auditory Brainstem Responses in Mouse Models |
| title_full | Quantitative Threshold Determination of Auditory Brainstem Responses in Mouse Models |
| title_fullStr | Quantitative Threshold Determination of Auditory Brainstem Responses in Mouse Models |
| title_full_unstemmed | Quantitative Threshold Determination of Auditory Brainstem Responses in Mouse Models |
| title_short | Quantitative Threshold Determination of Auditory Brainstem Responses in Mouse Models |
| title_sort | quantitative threshold determination of auditory brainstem responses in mouse models |
| topic | auditory brainstem responses (ABR) auditory function ABR threshold correlation coefficient cross-covariance |
| url | https://www.mdpi.com/1422-0067/24/14/11393 |
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