Human S100A9 protein is stabilized by inflammatory stimuli via the formation of proteolytically-resistant homodimers.
S100A8 and S100A9 are Ca(2+)-binding proteins that are associated with acute and chronic inflammation and cancer. They form predominantly heterodimers even if there are data supporting homodimer formation. We investigated the stability of the heterodimer in myeloid and S100A8/S100A9 over-expressing...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3633927?pdf=render |
| _version_ | 1818487336968650752 |
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| author | Matteo Riva Zhifei He Eva Källberg Fredrik Ivars Tomas Leanderson |
| author_facet | Matteo Riva Zhifei He Eva Källberg Fredrik Ivars Tomas Leanderson |
| author_sort | Matteo Riva |
| collection | DOAJ |
| description | S100A8 and S100A9 are Ca(2+)-binding proteins that are associated with acute and chronic inflammation and cancer. They form predominantly heterodimers even if there are data supporting homodimer formation. We investigated the stability of the heterodimer in myeloid and S100A8/S100A9 over-expressing COS cells. In both cases, S100A8 and S100A9 proteins were not completely degraded even 48 hrs after blocking protein synthesis. In contrast, in single transfected cells, S100A8 protein was completely degraded after 24 h, while S100A9 was completely unstable. However, S100A9 protein expression was rescued upon S100A8 co-expression or inhibition of proteasomal activity. Furthermore, S100A9, but not S100A8, could be stabilized by LPS, IL-1β and TNFα treatment. Interestingly, stimulation of S100A9-transfected COS cells with proteasomal inhibitor or IL-1β lead to the formation of protease resistant S100A9 homodimers. In summary, our data indicated that S100A9 protein is extremely unstable but can be rescued upon co-expression with S100A8 protein or inflammatory stimuli, via proteolytically resistant homodimer formation. The formation of S100A9 homodimers by this mechanism may constitute an amplification step during an inflammatory reaction. |
| first_indexed | 2024-12-10T16:36:30Z |
| format | Article |
| id | doaj.art-60ecd8b48f514e65b3cc2f26b45e8221 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-10T16:36:30Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-60ecd8b48f514e65b3cc2f26b45e82212022-12-22T01:41:24ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0184e6183210.1371/journal.pone.0061832Human S100A9 protein is stabilized by inflammatory stimuli via the formation of proteolytically-resistant homodimers.Matteo RivaZhifei HeEva KällbergFredrik IvarsTomas LeandersonS100A8 and S100A9 are Ca(2+)-binding proteins that are associated with acute and chronic inflammation and cancer. They form predominantly heterodimers even if there are data supporting homodimer formation. We investigated the stability of the heterodimer in myeloid and S100A8/S100A9 over-expressing COS cells. In both cases, S100A8 and S100A9 proteins were not completely degraded even 48 hrs after blocking protein synthesis. In contrast, in single transfected cells, S100A8 protein was completely degraded after 24 h, while S100A9 was completely unstable. However, S100A9 protein expression was rescued upon S100A8 co-expression or inhibition of proteasomal activity. Furthermore, S100A9, but not S100A8, could be stabilized by LPS, IL-1β and TNFα treatment. Interestingly, stimulation of S100A9-transfected COS cells with proteasomal inhibitor or IL-1β lead to the formation of protease resistant S100A9 homodimers. In summary, our data indicated that S100A9 protein is extremely unstable but can be rescued upon co-expression with S100A8 protein or inflammatory stimuli, via proteolytically resistant homodimer formation. The formation of S100A9 homodimers by this mechanism may constitute an amplification step during an inflammatory reaction.http://europepmc.org/articles/PMC3633927?pdf=render |
| spellingShingle | Matteo Riva Zhifei He Eva Källberg Fredrik Ivars Tomas Leanderson Human S100A9 protein is stabilized by inflammatory stimuli via the formation of proteolytically-resistant homodimers. PLoS ONE |
| title | Human S100A9 protein is stabilized by inflammatory stimuli via the formation of proteolytically-resistant homodimers. |
| title_full | Human S100A9 protein is stabilized by inflammatory stimuli via the formation of proteolytically-resistant homodimers. |
| title_fullStr | Human S100A9 protein is stabilized by inflammatory stimuli via the formation of proteolytically-resistant homodimers. |
| title_full_unstemmed | Human S100A9 protein is stabilized by inflammatory stimuli via the formation of proteolytically-resistant homodimers. |
| title_short | Human S100A9 protein is stabilized by inflammatory stimuli via the formation of proteolytically-resistant homodimers. |
| title_sort | human s100a9 protein is stabilized by inflammatory stimuli via the formation of proteolytically resistant homodimers |
| url | http://europepmc.org/articles/PMC3633927?pdf=render |
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