Biosynthesis of a sulfated exopolysaccharide, synechan, and bloom formation in the model cyanobacterium Synechocystis sp. strain PCC 6803
Extracellularpolysaccharides of bacteria contribute to biofilm formation, stress tolerance, and infectivity. Cyanobacteria, the oxygenic photoautotrophic bacteria, uniquely produce sulfated extracellular polysaccharides among bacteria to support phototrophic biofilms. In addition, sulfated polysacch...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2021-06-01
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| Series: | eLife |
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| Online Access: | https://elifesciences.org/articles/66538 |
| _version_ | 1811253305578356736 |
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| author | Kaisei Maeda Yukiko Okuda Gen Enomoto Satoru Watanabe Masahiko Ikeuchi |
| author_facet | Kaisei Maeda Yukiko Okuda Gen Enomoto Satoru Watanabe Masahiko Ikeuchi |
| author_sort | Kaisei Maeda |
| collection | DOAJ |
| description | Extracellularpolysaccharides of bacteria contribute to biofilm formation, stress tolerance, and infectivity. Cyanobacteria, the oxygenic photoautotrophic bacteria, uniquely produce sulfated extracellular polysaccharides among bacteria to support phototrophic biofilms. In addition, sulfated polysaccharides of cyanobacteria and other organisms have been focused as beneficial biomaterial. However, very little is known about their biosynthesis machinery and function in cyanobacteria. Here, we found that the model cyanobacterium, Synechocystis sp. strain PCC 6803, formed bloom-like cell aggregates embedded in sulfated extracellular polysaccharides (designated as synechan) and identified whole set of genes responsible for synechan biosynthesis and its transcriptional regulation, thereby suggesting a model for the synechan biosynthesis apparatus. Because similar genes are found in many cyanobacterial genomes with wide variation, our findings may lead elucidation of various sulfated polysaccharides, their functions, and their potential application in biotechnology. |
| first_indexed | 2024-04-12T16:48:54Z |
| format | Article |
| id | doaj.art-60ed646eaae848a8b58f72ad765b1d83 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T16:48:54Z |
| publishDate | 2021-06-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-60ed646eaae848a8b58f72ad765b1d832022-12-22T03:24:28ZengeLife Sciences Publications LtdeLife2050-084X2021-06-011010.7554/eLife.66538Biosynthesis of a sulfated exopolysaccharide, synechan, and bloom formation in the model cyanobacterium Synechocystis sp. strain PCC 6803Kaisei Maeda0Yukiko Okuda1Gen Enomoto2Satoru Watanabe3Masahiko Ikeuchi4https://orcid.org/0000-0003-4231-8423Department of Life Sciences (Biology), Graduate School of Arts and Sciences, University of Tokyo, Tokyo, JapanDepartment of Life Sciences (Biology), Graduate School of Arts and Sciences, University of Tokyo, Tokyo, JapanDepartment of Life Sciences (Biology), Graduate School of Arts and Sciences, University of Tokyo, Tokyo, JapanDepartment of Bioscience, Tokyo University of Agriculture, Tokyo, JapanDepartment of Life Sciences (Biology), Graduate School of Arts and Sciences, University of Tokyo, Tokyo, Japan; Faculty of Education and Integrated Arts and Sciences, Waseda University, Tokyo, JapanExtracellularpolysaccharides of bacteria contribute to biofilm formation, stress tolerance, and infectivity. Cyanobacteria, the oxygenic photoautotrophic bacteria, uniquely produce sulfated extracellular polysaccharides among bacteria to support phototrophic biofilms. In addition, sulfated polysaccharides of cyanobacteria and other organisms have been focused as beneficial biomaterial. However, very little is known about their biosynthesis machinery and function in cyanobacteria. Here, we found that the model cyanobacterium, Synechocystis sp. strain PCC 6803, formed bloom-like cell aggregates embedded in sulfated extracellular polysaccharides (designated as synechan) and identified whole set of genes responsible for synechan biosynthesis and its transcriptional regulation, thereby suggesting a model for the synechan biosynthesis apparatus. Because similar genes are found in many cyanobacterial genomes with wide variation, our findings may lead elucidation of various sulfated polysaccharides, their functions, and their potential application in biotechnology.https://elifesciences.org/articles/66538synechocystis sp. PCC 6803cyanobacteriaexopolysaccharidebloom |
| spellingShingle | Kaisei Maeda Yukiko Okuda Gen Enomoto Satoru Watanabe Masahiko Ikeuchi Biosynthesis of a sulfated exopolysaccharide, synechan, and bloom formation in the model cyanobacterium Synechocystis sp. strain PCC 6803 eLife synechocystis sp. PCC 6803 cyanobacteria exopolysaccharide bloom |
| title | Biosynthesis of a sulfated exopolysaccharide, synechan, and bloom formation in the model cyanobacterium Synechocystis sp. strain PCC 6803 |
| title_full | Biosynthesis of a sulfated exopolysaccharide, synechan, and bloom formation in the model cyanobacterium Synechocystis sp. strain PCC 6803 |
| title_fullStr | Biosynthesis of a sulfated exopolysaccharide, synechan, and bloom formation in the model cyanobacterium Synechocystis sp. strain PCC 6803 |
| title_full_unstemmed | Biosynthesis of a sulfated exopolysaccharide, synechan, and bloom formation in the model cyanobacterium Synechocystis sp. strain PCC 6803 |
| title_short | Biosynthesis of a sulfated exopolysaccharide, synechan, and bloom formation in the model cyanobacterium Synechocystis sp. strain PCC 6803 |
| title_sort | biosynthesis of a sulfated exopolysaccharide synechan and bloom formation in the model cyanobacterium synechocystis sp strain pcc 6803 |
| topic | synechocystis sp. PCC 6803 cyanobacteria exopolysaccharide bloom |
| url | https://elifesciences.org/articles/66538 |
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